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DNA binding assays

KM Kyle W Muir

Last updated date: Mar 26, 2021 Views: 880 Forks: 0

original An abbreviated version of this protocol was published in eLife in Aug, 2018
Structural basis for Scc3-dependent cohesin recruitment to chromatin
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Dear Laya,
                  Please find the full list DNA sequences below. Best of luck with your experiments!

Kyle

15bp.for: 5’ TAAACGAAAGTGAAC 3’

15b.rev: 5’ AGTTCACTTTCGTTT 3’

17bp.for: 5’ TTTTCAAGGAAACGAAA 3’

17bp.rev: 5’ ATTTCGTTTCCTTGAAA 3’

19bp.for: 5’ TTTTTCAAGGAAACGAAAG 3’

19bp.rev: 5’ ACTTTCGTTTCCTTGAAAA 3’

22bp.for: 5’ TCTTTTCAAGGAAACGAAAGTG 3’

22bp.rev: 5’ ACACTTTCGTTTCCTTGAAAAG 3’

25bp.for: 5’ TGCCTTTTCAAGGAAACGAAAGTGA 3’

25bp.rev: 5’ ATCACTTTCGTTTCCTTGAAAAGGC 3’

32bp.for: 5’ TGGAAGCCTTTTCAAGGAAACGAAAGTGAACT 3’

32bp.rev: 5’ AAGTTCACTTTCGTTTCCTTGAAAAGGCTTCC 3’

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Muir, K(2021). DNA binding assays. Bio-protocol Preprint. bio-protocol.org/prep975.
  2. Li, Y., Muir, K. W., Bowler, M. W., Metz, J., Haering, C. H. and Panne, D.(2018). Structural basis for Scc3-dependent cohesin recruitment to chromatin. eLife. DOI: 10.7554/eLife.38356

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