Protocols shared by authors via RaP service
Journal
Measurement of the labile iron pool
Author: Jumpei Ito
Date: Mar 5, 2021，Views：1600
Original protocol used in research article：
Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice
eLife, Feb 2, 2021; DOI: 10.7554/eLife.62174
crRNA selection
Authors: Francois Kroll and Jason Rihel
Date: Mar 5, 2021，Views：1241
Original protocol used in research article：
A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
eLife, Jan 8, 2021; DOI: 10.7554/eLife.59683
Information flow analysis
Author: Lucie Delemotte
Date: Mar 4, 2021，Views：760
Original protocol used in research article：
Calmodulin acts as a state-dependent switch to control a cardiac potassium channel opening
Science Advances, Dec 11, 2020; DOI: 10.1126/sciadv.abd6798
Lineage Labeling & Cell Transfer
Author: Bruce B Riley
Date: Mar 4, 2021，Views：812
Original protocol used in research article：
The Warburg Effect and lactate signaling augment Fgf-MAPK to promote sensory-neural development in the otic vesicle
eLife, Apr 27, 2020; DOI: 10.7554/eLife.56301
Data collection
Author: Melody G Campbell
Date: Mar 4, 2021，Views：949
Original protocol used in research article：
2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy
eLife, Mar 11, 2015; DOI: 10.7554/eLife.06380
ATAC-seq protocol
Authors: Haoze Vincent Yu and Neil Segil
Date: Mar 4, 2021，Views：901
Original protocol used in research article：
Foxc1 establishes enhancer accessibility for craniofacial cartilage differentiation
eLife, Jan 27, 2021; DOI: 10.7554/eLife.63595
Synthesis and characterization of TPT polymer, d-LND, and HA-DOX
Authors: Jun Cao and Huile Gao
Date: Mar 4, 2021，Views：953
Original protocol used in research article：
A combinational chemo-immune therapy using an enzyme-sensitive nanoplatform for dual-drug delivery to specific sites by cascade targeting
Science Advances, Feb 4, 2021; DOI: 10.1126/sciadv.aba0776
Cell lysis, IP, immunoblotting, and antibodies
Author: Yan-ping Xu
Date: Mar 3, 2021，Views：854
Original protocol used in research article：
USP15 suppresses tumor immunity via deubiquitylation and inactivation of TET2
Science Advances, Sep 17, 2020; DOI: 10.1126/sciadv.abc9730
Human Cytomegalovirus (HCMV) infection of CD14+ monocytes and reactivation
Authors: Miri Shnayder, Noam Stern-Ginossar and Michal Schwartz
Date: Mar 3, 2021，Views：859
Original protocol used in research article：
Single cell analysis reveals human cytomegalovirus drives latently infected cells towards an anergic-like monocyte state
eLife, Jan 22, 2020; DOI: 10.7554/eLife.52168
Preparation of BS biofilms
Author: Jinyao Liu
Date: Mar 3, 2021，Views：874
Original protocol used in research article：
Bioinspired oral delivery of gut microbiota by self-coating with biofilms
Science Advances, Jun 23, 2020; DOI: 10.1126/sciadv.abb1952
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