Protocols shared by authors via RaP service
Journal
Membrane protein detection
Authors: Shao-Cong Sun and Xiaofei Zhou
Date: Jun 28, 2021，Views：829
Original protocol used in research article：
The deubiquitinase Otub1 controls the activation of CD8 T cells and NK cells by regulating IL-15-mediated priming
Nat Immunol, Jun 10, 2019; DOI: 10.1038/s41590-019-0405-2
FluoroSpot Assay for ASCs
Authors: Renata Varnaitė and Sara Gredmark-Russ
Date: Jun 28, 2021，Views：811
Original protocol used in research article：
Expansion of SARS-CoV-2–Specific Antibody-Secreting Cells and Generation of Neutralizing Antibodies in Hospitalized COVID-19 Patients
J Immunol, Sep 2, 2020; DOI: 10.4049/jimmunol.2000717
In vitro Suppression Assay and Transmigration Assay
Authors: Ivan Koprivica and Ivana Stojanović
Date: Jun 28, 2021，Views：834
Original protocol used in research article：
Ethyl Pyruvate Stimulates Regulatory T Cells and Ameliorates Type 1 Diabetes Development in Mice
Frontiers in Immunology, Jan 10, 2019; DOI: 10.3389/fimmu.2018.03130
Ubiquitination assay
Authors: Qingchen Zhu and Yichuan Xiao
Date: Jun 28, 2021，Views：951
Original protocol used in research article：
Modulation of M2 macrophage polarization by the crosstalk between Stat6 and Trim24
Nature Communications, Sep 25, 2019; DOI: 10.1038/s41467-019-12384-2
In vitro Infection of Neutrophils With Leishmania donovani Promastigotes
Authors: Mareike Ohms, Tamás Laskay and Sonja Möller
Date: Jun 27, 2021，Views：765
Original protocol used in research article：
An Attempt to Polarize Human Neutrophils Toward N1 and N2 Phenotypes in vitro
Frontiers in Immunology, Apr 28, 2020; DOI: 10.3389/fimmu.2020.00532
In vitro SIRT6 deacetylation assay
Authors: Minxian Qian and Baohua Liu
Date: Jun 27, 2021，Views：882
Original protocol used in research article：
Deacetylase-independent function of SIRT6 couples GATA4 transcription factor and epigenetic activation against cardiomyocyte apoptosis
Nucleic Acids Research, Apr 2, 2020; DOI: 10.1093/nar/gkaa214
In utero electroporation of mouse embryos
Author: Wieland B Huttner
Date: Jun 26, 2021，Views：1006
Original protocol used in research article：
Extracellular matrix-inducing Sox9 promotes both basal progenitor proliferation and gliogenesis in developing neocortex
eLife, Mar 19, 2020; DOI: 10.7554/eLife.49808
Cell-free BoDV preparation.
Authors: Keizo Tomonaga and Akiko Makino
Date: Jun 25, 2021，Views：778
Original protocol used in research article：
ADAR2 Is Involved in Self and Nonself Recognition of Borna Disease Virus Genomic RNA in the Nucleus
Journal of Virology, Feb 28, 2020; DOI: 10.1128/JVI.01513-19
AAV vector production
Authors: Sean K Wang and Constance L. Cepko
Date: Jun 25, 2021，Views：900
Original protocol used in research article：
Microglia modulation by TGF-β1 protects cones in mouse models of retinal degeneration
The Journal of Clinical Investigation, Jul 13, 2020; DOI: 10.1172/JCI136160
Primary neuron-astrocyte co-culture
Authors: Michelle L Olsen and Raymundo D Hernandez
Date: Jun 25, 2021，Views：852
Original protocol used in research article：
Astrocyte morphogenesis is dependent on BDNF signaling via astrocytic TrkB.T1
eLife, Aug 21, 2019; DOI: 10.7554/eLife.44667
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