Protocols shared by authors via RaP service
Journal
Tracer injections
Authors: Natasha O'Brown, Sean G Megason and Chenghua Gu
Date: Sep 12, 2025，Views：0
Original protocol used in research article：
Suppression of transcytosis regulates zebrafish blood-brain barrier function
eLife, Aug 20, 2019; DOI: 10.7554/eLife.47326
Principle component analysis (PCA)
Authors: Kingsley Wu, Ta I Hung and Chia-en A Chang
Date: Sep 11, 2025，Views：0
Original protocol used in research article：
PROTAC-induced protein structural dynamics in targeted protein degradation
eLife, Feb 27, 2025; DOI: 10.7554/eLife.101127
ChIP-seq analysis
Authors: Liang Xu, Ye Chen and Tuan Zea Tan
Date: Sep 9, 2025，Views：0
Original protocol used in research article：
Topography of transcriptionally active chromatin in glioblastoma
Science Advances, Apr 30, 2021; DOI: 10.1126/sciadv.abd4676
Liposome reconstitution
Authors: David N Nathan Tippett and Raimund Dutzler
Date: Aug 26, 2025，Views：0
Original protocol used in research article：
Structural and functional properties of the transporter SLC26A6 reveal mechanism of coupled anion exchange
eLife, Jun 23, 2023; DOI: 10.7554/eLife.87178
Identification of putative enhancers
Authors: Xiaotong Wu and Wei Xie
Date: Aug 22, 2025，Views：0
Original protocol used in research article：
Methylome inheritance and enhancer dememorization reset an epigenetic gate safeguarding embryonic programs
Science Advances, Dec 22, 2021; DOI: 10.1126/sciadv.abl3858
Microinjection into mid-gestation E8.5 embryos
Author: Qiang Huang
Date: Aug 14, 2025，Views：2
Original protocol used in research article：
Intravital imaging of mouse embryos
Science, Apr 9, 2020; DOI: 10.1126/science.aba0210
Preparation of retinol, RBP, and TTR
Author: Regina M Murphy
Date: Aug 14, 2025，Views：7
Original protocol used in research article：
An in vitro model for vitamin A transport across the human blood–brain barrier
eLife, Nov 7, 2023; DOI: 10.7554/eLife.87863
Fluorescent BODIPY-FL vancomycin staining
Authors: Moagi Tube Shaku and Bavesh D Kana
Date: Aug 8, 2025，Views：16
Original protocol used in research article：
A modified BCG with depletion of enzymes associated with peptidoglycan amidation induces enhanced protection against tuberculosis in mice
eLife, Apr 19, 2024; DOI: 10.7554/eLife.89157
Detection of CMV pp65-specific T cells by intracellular staining with anti-INF-γ-PE
Author: David Klatzmann
Date: Aug 1, 2025，Views：35
Original protocol used in research article：
Human thymopoiesis produces polyspecific CD8+ α/β T cells responding to multiple viral antigens
eLife, Mar 30, 2023; DOI: 10.7554/eLife.81274
EdU immunostaining to detect dividing cells
Author: Richard W Naylor
Date: Jul 29, 2025，Views：83
Original protocol used in research article：
A novel mechanism of gland formation in zebrafish involving transdifferentiation of renal epithelial cells and live cell extrusion
eLife, Nov 5, 2018; DOI: 10.7554/eLife.38911
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