真菌

分类

    现刊
    An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated Protein
    利用芽殖酵母微管相关蛋白研究微管结合和微管交联的体外显微镜检测法
    作者:Yili Zhu, Weimin Tan and Wei-Lih Lee日期:12/05/2018,浏览量:169,Q&A: 0
    In this protocol, we describe a simple microscopy-based method to assess the interaction of a microtubule-associated protein (MAP) with microtubules. The interaction between MAP and microtubules is typically assessed by a co-sedimentation assay, ...
    Field Inoculation and Classification of Maize Ear Rot Caused by Fusarium verticillioides
    田间接种和轮枝镰孢菌引起的玉米穗粒腐病的分类
    作者:Chaopei Dong, Yabin Wu, Jingyang Gao, Zijian Zhou, Cong Mu, Peipei Ma, Jiafa Chen and Jianyu Wu日期:12/05/2018,浏览量:57,Q&A: 0
    Maize ear rot is a worldwide fungal disease mainly caused by Fusarium verticillioides and Fusarium graminearum. Maize planted in the field was inoculated with Fusarium verticillioides at the filling stage, 15 days after ...
    An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated Protein
    利用芽殖酵母微管相关蛋白研究微管结合和微管交联的体外显微镜检测法
    作者:Yili Zhu, Weimin Tan and Wei-Lih Lee日期:12/05/2018,浏览量:169,Q&A: 0
    [Abstract] In this protocol, we describe a simple microscopy-based method to assess the interaction of a microtubule-associated protein (MAP) with microtubules. The interaction between MAP and microtubules is typically assessed by a co-sedimentation assay, which measures the amount of MAP that co-pellets with microtubules by centrifugation, followed by ...
    Field Inoculation and Classification of Maize Ear Rot Caused by Fusarium verticillioides
    田间接种和轮枝镰孢菌引起的玉米穗粒腐病的分类
    作者:Chaopei Dong, Yabin Wu, Jingyang Gao, Zijian Zhou, Cong Mu, Peipei Ma, Jiafa Chen and Jianyu Wu日期:12/05/2018,浏览量:57,Q&A: 0
    [Abstract] Maize ear rot is a worldwide fungal disease mainly caused by Fusarium verticillioides and Fusarium graminearum. Maize planted in the field was inoculated with Fusarium verticillioides at the filling stage, 15 days after pollination. Two milliliters of spore suspension with a concentration of 5 x 106/ml was ...
    Separation and Visualization of Low Abundant Ubiquitylated Forms
    低丰度泛素化形式蛋白的分离和可视化
    作者:Ramona Schuster, Tânia Simões, Fabian den Brave and Mafalda Escobar-Henriques日期:11/20/2018,浏览量:449,Q&A: 0
    [Abstract] In this protocol we describe the separation and visualization of ubiquitylated forms of the yeast mitofusin Fzo1 by Western blot. To this aim, we express HA-tagged Fzo1 in Saccharomyces cerevisiae, break the cells to extract a membrane-enriched fraction, solubilize the membranes using detergent and then specifically immunoprecipitate the ...
    Preparation and Purification of Proteins Secreted from Phytophthora sojae
    大豆疫霉菌分泌蛋白的制备和纯化
    作者:Yeqiang Xia, Yan Wang and Yuanchao Wang日期:10/20/2018,浏览量:504,Q&A: 0
    [Abstract] Phytophthora sojae, the causal agent of soybean root and stem rot, is responsible for enormous economic losses in soybean production. P. sojae secrets various effectors to reprogram host immunity. The plant apoplastic space is a major battleground in plant-pathogen interactions. Here we describe a protocol for purification and ...
    Preparation of Sequencing RNA Libraries through Chemical Cross-linking Coupled to Affinity Purification (cCLAP) in Saccharomyces cerevisiae
    通过化学交联联合亲和纯化(cCLAP) 技术制备酿酒酵母RNA测序文库
    作者:Congwei Wang, Julie Weidner and Anne Spang日期:10/05/2018,浏览量:629,Q&A: 0
    [Abstract] Ribonucleoprotein particles (mRNPs) are complexes consisting of mRNAs and RNA-binding proteins (RBPs) which control mRNA transcription localization, turnover, and translation. Some mRNAs within the mRNPs have been shown to undergo degradation or storage. Those transcripts can lack general mRNA elements, like the poly(A) tail or 5’ cap structure, ...
    Identifying Protein Interactions with Histone Peptides Using Bio-layer Interferometry
    利用生物膜层干涉技术鉴定蛋白质和组蛋白肽之间相互作用
    作者:Bingbing Ren, Ahmed Mahmoud Mohammed Sayed, Hwei Ling Tan, Yu Keung Mok and Ee Sin Chen日期:09/20/2018,浏览量:578,Q&A: 0
    [Abstract] Histone post-translational modifications (PTMs) regulate numerous cellular processes, including gene transcription, cell division, and DNA damage repair. Most histone PTMs affect the recruitment or exclusion of reader proteins from chromatin. Here, we present a protocol to measure affinity and interaction kinetics between histone peptides and the ...
    Extracting and Integrating Protein Localization Changes from Multiple Image Screens of Yeast Cells
    利用多重图像筛选技术提取和整合酵母细胞蛋白定位变化
    作者:Alex X Lu, Louis-Francois Handfield and Alan M Moses日期:09/20/2018,浏览量:421,Q&A: 0
    [Abstract] The evaluation of protein localization changes in cells under diverse chemical and genetic perturbations is now possible due to the increasing quantity of screens that systematically image thousands of proteins in an organism. Integrating information from different screens provides valuable contextual information about the protein function. For ...
    Detection of Internal Matrix Targeting Signal-like Sequences (iMTS-Ls) in Mitochondrial Precursor Proteins Using the TargetP Prediction Tool
    用TargetP预测工具在线粒体前体蛋白中检测内部类基质导向信号序列
    作者:Felix Boos, Timo Mühlhaus and Johannes M. Herrmann日期:09/05/2018,浏览量:466,Q&A: 0
    [Abstract] Mitochondria contain hundreds of proteins which are encoded by the nuclear genome and synthesized in the cytosol from where they are imported into the organelle. Sorting signals encoded in the primary and secondary sequence of these proteins mediate the recognition of newly synthesized precursor proteins and their subsequent translocation through ...
    Artificial Inoculation of Epichloë festucae into Lolium perenne, and Visualisation of Endophytic and Epiphyllous Fungal Growth
    人工接种羊茅香柱菌到麦黑草,并可视化观察内生真菌和叶生真菌的生长
    作者:Yvonne Becker, Kimberly A. Green, Barry Scott and Matthias Becker日期:09/05/2018,浏览量:471,Q&A: 0
    [Abstract] Natural hosts for the fungal endophyte Epichloë festucae include Festuca rubra (fine fescue) and Festuca trachyphylla (hard fescue). Some strains also form stable associations with Lolium perenne (perennial ryegrass). L. perenne is a suitable host to study fungal endophyte–grass interactions, such as endophytic ...
    In vivo and in vitro 31P-NMR Study of the Phosphate Transport and Polyphosphate Metabolism in Hebeloma cylindrosporum in Response to Plant Roots Signals
    利用31P-NMR技术体内外研究柱孢白桦在响应植物根组织信号后的磷酸盐转运和多聚磷酸盐代谢
    [Abstract] We used in vivo and in vitro phosphorus-31 nuclear magnetic resonance (31P-NMR) spectroscopy to follow the change in transport, compartmentation and metabolism of phosphate in the ectomycorrhizal fungus Hebeloma cylindrosporum in response to root signals originating from host (Pinus pinaster) or ...