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真菌

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    现刊
    Establishing a Symbiotic Interface between Cultured Ectomycorrhizal Fungi and Plants to Follow Fungal Phosphate Metabolism
    在培养的外生菌根真菌和植物之间建立共生界面以示踪真菌磷酸盐代谢
    [Abstract] In ectomycorrhizal plants, the fungal cells colonize the roots of their host plant to create new organs called ectomycorrhizae. In these new organs, the fungal cells colonize the walls of the cortical cells, bathing in the same apoplasm as the plant cells in a space named the ‘Hartig net’, where exchanges between the two partners take place. ...
    A Method for Radioactive Labelling of Hebeloma cylindrosporum to Study Plant-fungus Interactions
    一种用于研究植物-真菌相互作用的圆形孢子粘花菇放射性标记方法
    [Abstract] In order to quantify P accumulation and P efflux in the ectomycorrhizal basidiomycete fungus Hebeloma cylindrosporum, we supplied 32P to mycelia previously grown in vitro in liquid medium. The culture had four main steps that are 1) growing the mycelium on complete medium with P, 2) transfer the mycelia into new ...
    Protein Expression and Purification of the Hsp90-Cdc37-Cdk4 Kinase Complex from Saccharomyces cerevisiae
    Hsp90-Cdc37-Cdk4激酶复合物在酿酒酵母中的表达和纯化
    作者:Kliment A. Verba and David A. Agard日期:10/05/2017,浏览量:1005,Q&A: 0
    [Abstract] Interactions between Hsp90, its co-chaperone Cdc37 and kinases have been biochemically studied for over three decades and have been shown to be functionally important in organisms from yeast to humans. However, formation of a stable complex for structural studies has been elusive. In this protocol we describe expression and purification of ...
    Large-scale Maize Seedling Infection with Exserohilum turcicum in the Greenhouse
    温室中大规模玉米幼苗感染玉米大斑病菌
    作者:Ping Yang, Gerhard Herren, Simon G. Krattinger and Beat Keller日期:10/05/2017,浏览量:976,Q&A: 0
    [Abstract] Northern corn leaf blight (NCLB) is a serious foliar disease of maize (Zea mays) worldwide and breeding for resistance is of primary importance for maize crop protection. Phenotyping for NCLB resistance is well established in the field, but such experiments depend on suitable environmental conditions and are seasonal. Here we describe a ...
    Accurate, Streamlined Analysis of mRNA Translation by Sucrose Gradient Fractionation
    通过蔗糖梯度分级分离准确、高效分析mRNA的翻译
    作者:Soufiane Aboulhouda, Rachael Di Santo, Gabriel Therizols and David Weinberg日期:10/05/2017,浏览量:1111,Q&A: 0
    [Abstract] The efficiency with which proteins are produced from mRNA molecules can vary widely across transcripts, cell types, and cellular states. Methods that accurately assay the translational efficiency of mRNAs are critical to gaining a mechanistic understanding of post-transcriptional gene regulation. One way to measure translational efficiency is to ...
    A Flow-assay for Farnesol Removal from Adherent Candida albicans Cultures
    从贴壁白色念珠菌培养物中去除法尼醇的流式实验
    作者:Melanie Polke and Ilse D. Jacobsen日期:10/05/2017,浏览量:715,Q&A: 0
    [Abstract] Here, we describe a protocol for a continuous flow system for C. albicans cultures growing adherent to a plastic surface. The protocol was adapted from a previous method established to simulate blood flow on endothelial cells (Wilson and Hube, 2010). The adapted protocol was used by us for the removal of molecules in C. albicans ...
    In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
    真核先导链/后随链DNA复制的体外分析
    作者:Grant Schauer, Jeff Finkelstein and Mike O’Donnell日期:09/20/2017,浏览量:1170,Q&A: 0
    [Abstract] The eukaryotic replisome is a multiprotein complex that duplicates DNA. The replisome is sculpted to couple continuous leading strand synthesis with discontinuous lagging strand synthesis, primarily carried out by DNA polymerases ε and δ, respectively, along with helicases, polymerase α-primase, DNA sliding clamps, clamp loaders and many other ...
    Freeze-fracture-etching Electron Microscopy for Facile Analysis of Yeast Ultrastructure
    电镜冷冻断裂蚀刻技术用于酵母超微结构的简易分析
    作者:Takuma Tsuji and Toyoshi Fujimoto日期:09/20/2017,浏览量:1174,Q&A: 0
    [Abstract] We describe a streamlined method that enables the quick observation of yeast ultrastructure by electron microscopy (EM). Yeast cells are high-pressure frozen, freeze-fractured to cut across the cytoplasm, and freeze-etched to sublimate ice in the cytosol and the organelle lumen. The cellular structures delineated by these procedures are coated by ...
    Method for Multiplexing CRISPR/Cas9 in Saccharomyces cerevisiae Using Artificial Target DNA Sequences
    酿酒酵母中使用人工靶DNA序列进行多重CRISPR/ Cas9的方法
    作者:Rachael M. Giersch and Gregory C. Finnigan日期:09/20/2017,浏览量:1489,Q&A: 0
    [Abstract] Genome manipulation has become more accessible given the advent of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) editing technology. The Cas9 endonuclease binds a single stranded (single guide) RNA (sgRNA) fragment that recruits the complex to a corresponding genomic target sequence where it induces a double stranded ...
    Sterol Analysis in Kluyveromyces lactis
    乳酸克鲁维酵母中的甾醇分析
    作者:Yvetta Gbelska, Nora Toth Hervay, Marcela Morvova and Alexandra Konecna日期:09/05/2017,浏览量:669,Q&A: 0
    [Abstract] Sterols are essential lipids of most eukaryotic cells with multiple functions (structural, regulatory and developmental). Sterol profile of yeast cells is often determined during the studies of ergosterol synthesis mutants used to uncover a number of functions for various sterols in yeast cells. Molecular studies of ergosterol biosynthesis have ...