Protocols shared by authors via RaP service
Journal
Purification of recombinant bacterial proteins
Authors: Min Young Kim and Chul Geun Kim
Date: Oct 31, 2021，Views：814
Original protocol used in research article：
Rational discovery of antimetastatic agents targeting the intrinsically disordered region of MBD2
Science Advances, Nov 19, 2019; DOI: 10.1126/sciadv.aav9810
Environmental Scanning Electron Microscopy (SEM)
Authors: Lucie Riglet, Isabelle Fobis-Loisy and Thierry Gaude
Date: Oct 14, 2021，Views：880
Original protocol used in research article：
KATANIN-dependent mechanical properties of the stigmatic cell wall mediate the pollen tube path in Arabidopsis
eLife, Sep 1, 2020; DOI: 10.7554/eLife.57282
MALDI-MS analysis
Authors: Yan Zhang, Joshua E Mayfield and Seema Irani
Date: Oct 11, 2021，Views：800
Original protocol used in research article：
Tyr1 phosphorylation promotes phosphorylation of Ser2 on the C-terminal domain of eukaryotic RNA polymerase II by P-TEFb
eLife, Aug 6, 2019; DOI: 10.7554/eLife.48725
Manufacturing of hOBMT
Authors: Nathalie Bock, Dietmar W. Hutmacher and Judith A. Clements
Date: Oct 10, 2021，Views：836
Original protocol used in research article：
In vitro engineering of a bone metastases model allows for study of the effects of antiandrogen therapies in advanced prostate cancer
Science Advances, Jun 30, 2021; DOI: 10.1126/sciadv.abg2564
Multifunctionality assessments
Authors: Marcel G. A. van der Heijden and Raphaël Albert Wittwer
Date: Oct 6, 2021，Views：790
Original protocol used in research article：
Organic and conservation agriculture promote ecosystem multifunctionality
Science Advances, Aug 20, 2021; DOI: 10.1126/sciadv.abg6995
PARP1 Purification Protocol
Authors: Pam Dyer, Johannes Rudolph and Karolin Luger
Date: Sep 29, 2021，Views：876
Original protocol used in research article：
Poly(ADP-ribose) polymerase 1 searches DNA via a ‘monkey bar’ mechanism
eLife, Aug 8, 2018; DOI: 10.7554/eLife.37818
Headplate and cranial window surgery for two-photon imaging of inferior colliculus in transgenic mice
Authors: Aaron Benson Wong and J Gerard G Borst
Date: Sep 28, 2021，Views：926
Original protocol used in research article：
Tonotopic and non-auditory organization of the mouse dorsal inferior colliculus revealed by two-photon imaging
eLife, Oct 15, 2019; DOI: 10.7554/eLife.49091
Generation of transgenic lines
Authors: Wenqing Zhang and Jin Xu
Date: Sep 13, 2021，Views：866
Original protocol used in research article：
Adult zebrafish Langerhans cells arise from hematopoietic stem/progenitor cells
eLife, Jun 15, 2018; DOI: 10.7554/eLife.36131
Immunoprecipitation of recombinant LyzC with His-SUMO-Cab45 purified from HEK293 supernatant
Authors: Mai Ly Tran, Bulat R Ramazanov, Tobias Karl-Heinz Hecht and Julia von Blume
Date: Sep 1, 2021，Views：824
Original protocol used in research article：
Fam20C regulates protein secretion by Cab45 phosphorylation
The Journal of Cell Biology, May 18, 2020; DOI: 10.1083/jcb.201910089
Immunofluorescence Analysis of GLUT1 Expression
Authors: Sameer Mohammed, Saeed Al Mahri and Bahauddeen M. Alrfaei
Date: Aug 28, 2021，Views：769
Original protocol used in research article：
Proteomic and Molecular Assessment of the Common Saudi Variant in ACADVL Gene Through Mesenchymal Stem Cells
Frontiers in Cell and Developmental Biology, Jan 10, 2020; DOI: 10.3389/fcell.2019.00365
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