Protocols shared by authors via RaP service
Journal
Electrophoretic mobility shift assays (EMSAs)
Authors: Roni M Lahr and Andrea J Berman
Date: Aug 2, 2022，Views：617
Original protocol used in research article：
La-related protein 1 (LARP1) binds the mRNA cap, blocking eIF4F assembly on TOP mRNAs
eLife, Apr 7, 2017; DOI: 10.7554/eLife.24146
qRT-PCR
Authors: Alok Kumar and Tasuku Honjo
Date: Aug 2, 2022，Views：587
Original protocol used in research article：
Tumors attenuating the mitochondrial activity in T cells escape from PD-1 blockade therapy
eLife, Mar 3, 2020; DOI: 10.7554/eLife.52330
Bilateral tumor model
Authors: Alok Kumar, Kenji Chamoto and Tasuku Honjo
Date: Aug 2, 2022，Views：632
Original protocol used in research article：
Tumors attenuating the mitochondrial activity in T cells escape from PD-1 blockade therapy
eLife, Mar 3, 2020; DOI: 10.7554/eLife.52330
Monotherapy model using anti-PD-L1 antibody [Cell number]
Authors: Alok Kumar, Kenji Chamoto and Tasuku Honjo
Date: Aug 2, 2022，Views：670
Original protocol used in research article：
Tumors attenuating the mitochondrial activity in T cells escape from PD-1 blockade therapy
eLife, Mar 3, 2020; DOI: 10.7554/eLife.52330
Cryo-EM data processing
Authors: Raimund Dutzler and Monique Sarah Straub
Date: Aug 2, 2022，Views：624
Original protocol used in research article：
Cryo-EM structures of the caspase-activated protein XKR9 involved in apoptotic lipid scrambling
eLife, Jul 15, 2021; DOI: 10.7554/eLife.69800
Embryo microinjection and mutation screening
Authors: Omar S Akbari and Ming Li
Date: Aug 1, 2022，Views：576
Original protocol used in research article：
Development of a confinable gene drive system in the human disease vector Aedes aegypti
eLife, Jan 21, 2020; DOI: 10.7554/eLife.51701
Exogenous ecdysone feeding treatments
Authors: Alexander Shingleton and Christen K Mirth
Date: Aug 1, 2022，Views：676
Original protocol used in research article：
Ecdysone coordinates plastic growth with robust pattern in the developing wing
eLife, Mar 9, 2022; DOI: 10.7554/eLife.72666
3.10. Flow Cytometry-Based Proliferation Assays
Authors: Jiayao Fu, Chuangqi Yu and Lingyan Zheng
Date: Aug 1, 2022，Views：573
Original protocol used in research article：
Pharmacological Inhibition of Glutaminase 1 Normalized the Metabolic State and CD4+ T Cell Response in Sjogren's Syndrome
Journal of Immunology Research, Feb 15, 2022; DOI: 10.1155/2022/3210200
ELISA and real-time PCR
Authors: Gilad Barshad, Elia D. Tait Wojno and Lauren Webb
Date: Aug 1, 2022，Views：568
Original protocol used in research article：
E-Protein Inhibition in ILC2 Development Shapes the Function of Mature ILC2s during Allergic Airway Inflammation
J Immunol, Mar 1, 2022; DOI: 10.4049/jimmunol.2100414
Preparation of pancreatic duct cells for single-cell analysis
Authors: Audrey M Hendley and Matthias Hebrok
Date: Jul 31, 2022，Views：589
Original protocol used in research article：
Single-cell transcriptome analysis defines heterogeneity of the murine pancreatic ductal tree
eLife, May 19, 2021; DOI: 10.7554/eLife.67776
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