Protocols shared by authors via RaP service
Journal
PKG-dependent TSC2 phosphorylation
Author: David A Kass
Date: Aug 5, 2022，Views：545
Original protocol used in research article：
Single serine on TSC2 exerts biased control over mTORC1 activation mediated by ERK1/2 but not Akt
Life Science Alliance, Mar 14, 2022; DOI: 10.26508/lsa.202101169
Primary source gender and cell line confirmations
Authors: Eben G Estell and Clifford J Rosen
Date: Aug 5, 2022，Views：553
Original protocol used in research article：
Irisin directly stimulates osteoclastogenesis and bone resorption in vitro and in vivo
eLife, Aug 11, 2020; DOI: 10.7554/eLife.58172
ISH and IHC
Authors: Minh Q Nguyen, Nicholas JP Ryba and Steve Davidson
Date: Aug 5, 2022，Views：587
Original protocol used in research article：
Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons
eLife, Nov 26, 2021; DOI: 10.7554/eLife.71752
Test on generated test data
Authors: Sebastian Fürthauer and Stefanie Redemann
Date: Aug 4, 2022，Views：567
Original protocol used in research article：
Microtubule reorganization during female meiosis in C. elegans
eLife, Jun 11, 2021; DOI: 10.7554/eLife.58903
Exercise training
Authors: Alyson Sujkowski, Robert J Wessells and Sokol V Todi
Date: Aug 4, 2022，Views：647
Original protocol used in research article：
Endurance exercise ameliorates phenotypes in Drosophila models of spinocerebellar ataxias
eLife, Feb 16, 2022; DOI: 10.7554/eLife.75389
Calculating David's Score
Authors: Katherine B LeClair and Scott J Russo
Date: Aug 2, 2022，Views：923
Original protocol used in research article：
Individual history of winning and hierarchy landscape influence stress susceptibility in mice
eLife, Sep 28, 2021; DOI: 10.7554/eLife.71401
HiCRep analysis
Authors: Sarah G Swygert and Toshio Tsukiyama
Date: Aug 2, 2022，Views：527
Original protocol used in research article：
Local chromatin fiber folding represses transcription and loop extrusion in quiescent cells
eLife, Nov 4, 2021; DOI: 10.7554/eLife.72062
Electrophoretic mobility shift assays (EMSAs)
Authors: Roni M Lahr and Andrea J Berman
Date: Aug 2, 2022，Views：586
Original protocol used in research article：
La-related protein 1 (LARP1) binds the mRNA cap, blocking eIF4F assembly on TOP mRNAs
eLife, Apr 7, 2017; DOI: 10.7554/eLife.24146
qRT-PCR
Authors: Alok Kumar and Tasuku Honjo
Date: Aug 2, 2022，Views：567
Original protocol used in research article：
Tumors attenuating the mitochondrial activity in T cells escape from PD-1 blockade therapy
eLife, Mar 3, 2020; DOI: 10.7554/eLife.52330
Bilateral tumor model
Authors: Alok Kumar, Kenji Chamoto and Tasuku Honjo
Date: Aug 2, 2022，Views：603
Original protocol used in research article：
Tumors attenuating the mitochondrial activity in T cells escape from PD-1 blockade therapy
eLife, Mar 3, 2020; DOI: 10.7554/eLife.52330
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.