Bio-101 - Improve Research Reproducibility

Population structure and signatures of selection

Author: Luke Thomas
Date: Aug 18, 2022，Views：534

Original protocol used in research article：

Spatially varying selection between habitats drives physiological shifts and local adaptation in a broadcast spawning coral on a remote atoll in Western Australia
Science Advances, Apr 27, 2022; DOI: 10.1126/sciadv.abl9185

Does TRH increase LSNA or BAT SNA via actions in the DMH and/or RPa?

Authors: Zhigang Shi, Daniel L Marks and Virginia L Brooks
Date: Aug 18, 2022，Views：525

Original protocol used in research article：

Leptin increases sympathetic nerve activity via induction of its own receptor in the paraventricular nucleus
eLife, Jun 15, 2020; DOI: 10.7554/eLife.55357

FtsZ and GpsB purification

Authors: Prahathees J Eswara and Kumaran S Ramamurthi
Date: Aug 18, 2022，Views：543

Original protocol used in research article：

An essential Staphylococcus aureus cell division protein directly regulates FtsZ dynamics
eLife, Oct 2, 2018; DOI: 10.7554/eLife.38856

TraDIS method.

Authors: Karthik Pullela, Ian R. Henderson, Alessandra Polissi and Waldemar Vollmer
Date: Aug 17, 2022，Views：527

Original protocol used in research article：

DpaA Detaches Braun’s Lipoprotein from Peptidoglycan
mBio, May 4, 2021; DOI: 10.1128/mbio.00836-21

In vitro culture on tissue culture plastic, fibroblasts, or keratinocytes

Authors: Tiago R. Matos, Jessica E. Teague and Rachael A. Clark
Date: Aug 16, 2022，Views：580

Original protocol used in research article：

Central memory T cells are the most effective precursors of resident memory T cells in human skin
Science Immunology, Apr 22, 2022; DOI: 10.1126/sciimmunol.abn1889

Diversity based on T cell composition

Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Aug 15, 2022，Views：578

Original protocol used in research article：

Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474

Indisulam efficacy studies in in vivo models

Authors: Shivendra Singh, Waise Quarni, Deepak Nijhawan, Andrew M. Davidoff and Jun Yang
Date: Aug 15, 2022，Views：538

Original protocol used in research article：

Targeting the spliceosome through RBM39 degradation results in exceptional responses in high-risk neuroblastoma models
Science Advances, Nov 17, 2021; DOI: 10.1126/sciadv.abj5405

In vitro phase separation assay

Authors: Yu Zhang, Lisha Shen and Hao Yu
Date: Aug 14, 2022，Views：626

Original protocol used in research article：

Phase separation of HRLP regulates flowering time in Arabidopsis
Science Advances, Jun 22, 2022; DOI: 10.1126/sciadv.abn5488

Plaque assays and immunostaining.

Authors: Chengjin Ye and Luis Martinez-Sobrido
Date: Aug 12, 2022，Views：548

Original protocol used in research article：

Generation and Characterization of Recombinant SARS-CoV-2 Expressing Reporter Genes
Journal of Virology, Mar 10, 2021; DOI: 10.1128/JVI.02209-20

RNA sequencing, RNA immunoprecipitation, and plasmid barcode sequencing

Authors: James Hose and Audrey P Gasch
Date: Aug 12, 2022，Views：532

Original protocol used in research article：

The genetic basis of aneuploidy tolerance in wild yeast
eLife, Jan 7, 2020; DOI: 10.7554/eLife.52063

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