Protocols shared by authors via RaP service
Journal
Detection of phosphoepitopes by Flow cytometry
Authors: Antoine Marçais and Thierry Walzer
Date: Nov 7, 2022，Views：611
Original protocol used in research article：
High mTOR activity is a hallmark of reactive natural killer cells and amplifies early signaling through activating receptors
eLife, Sep 6, 2017; DOI: 10.7554/eLife.26423
ENCODE dynamic k-mer–assisted mapping
Authors: Ariel Gershman, Michael E. G. Sauria, Karen H. Miga and Winston Timp
Date: Nov 7, 2022，Views：493
Original protocol used in research article：
Epigenetic patterns in a complete human genome
Science, Apr 1, 2022; DOI: 10.1126/science.abj5089
Repeated stimulation assay
Authors: Giedre Krenciute, Stephen Gottschalk and Ben Youngblood
Date: Nov 6, 2022，Views：659
Original protocol used in research article：
Deleting DNMT3A in CAR T cells prevents exhaustion and enhances antitumor activity
Science Translational Medicine, Nov 17, 2021; DOI: 10.1126/scitranslmed.abh0272
pMHCII-CAR constructs
Authors: Jaeu Yi, Gregory F. Wu and Chyi-Song Hsieh
Date: Nov 5, 2022，Views：584
Original protocol used in research article：
Antigen-specific depletion of CD4+ T cells by CAR T cells reveals distinct roles of higher- and lower-affinity TCRs during autoimmunity
Science Immunology, Oct 7, 2022; DOI: 10.1126/sciimmunol.abo0777
Microtubule co-sedimentation assay
Authors: Shaohe Wang and Karen Oegema
Date: Nov 4, 2022，Views：738
Original protocol used in research article：
NOCA-1 functions with γ-tubulin and in parallel to Patronin to assemble non-centrosomal microtubule arrays in C. elegans
eLife, Sep 15, 2015; DOI: 10.7554/eLife.08649
Protein expression and purification of OCT4 and SOX2
Authors: Dirk Schübeler and Nicolas H. Thomä
Date: Nov 3, 2022，Views：560
Original protocol used in research article：
Mechanisms of OCT4-SOX2 motif readout on nucleosomes
Science, Jun 25, 2020; DOI: 10.1126/science.abb0074
RNA-seq and read mapping
Authors: Mao-Bin Xie and Ming Liu
Date: Nov 2, 2022，Views：416
Original protocol used in research article：
Targeting tumor lineage plasticity in hepatocellular carcinoma using an anti-CLDN6 antibody-drug conjugate
Science Translational Medicine, Feb 3, 2021; DOI: 10.1126/scitranslmed.abb6282
GFP-VLPs and flow cytometry
Authors: Abdullah M. Syed, Melanie Ott and Jennifer A. Doudna
Date: Nov 2, 2022，Views：507
Original protocol used in research article：
Rapid assessment of SARS-CoV-2 evolved variants using virus-like particles
Science, Nov 4, 2021; DOI: 10.1126/science.abl6184
Fluorescence microscopy
Author: Benjamin S Glick
Date: Nov 2, 2022，Views：372
Original protocol used in research article：
COPI selectively drives maturation of the early Golgi
eLife, Dec 28, 2015; DOI: 10.7554/eLife.13232
Immunofluorescence and whole mount aorta imaging
Authors: Marco Orecchioni and Klaus Ley
Date: Nov 2, 2022，Views：362
Original protocol used in research article：
Olfactory receptor 2 in vascular macrophages drives atherosclerosis by NLRP3-dependent IL-1 production
Science, Jan 13, 2022; DOI: 10.1126/science.abg3067
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