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Tracing of visual projections
Last updated date: Jan 16, 2023 Views: 424 Forks: 0
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Dear Carlos,
For any species (mice, fish), we used AlexaFluor555-CTB C22843 or AlexaFluor647-CTB C34778 at 0.2 % diluted in water. We then aliquot in 0.5 ml microtubes and store them at -20°C. As we're using a lot of CTB, we never keep them at -20°C more than a few months.
Kim
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
- Vigouroux, R, Chédotal, A and Nguyen-Ba-Charvet, K(2023). Tracing of visual projections. Bio-protocol Preprint. bio-protocol.org/prep2119.
- Vigouroux, R. J., Cesar, Q., Chédotal, A. and Nguyen-Ba-Charvet, K. T.(2020). Revisiting the role of Dcc in visual system development with a novel eye clearing method. eLife. DOI: 10.7554/eLife.51275
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