Protocols shared by authors via RaP service
Journal
Lipofectamine transfection of organoids
Authors: Dawei Sun and Emma L Rawlins
Date: Dec 14, 2022，Views：1437
Original protocol used in research article：
A functional genetic toolbox for human tissue-derived organoids
eLife, Oct 6, 2021; DOI: 10.7554/eLife.67886
The Intolerance of Uncertainty Scale – Short Form (IUS-12)
Authors: Einav Gozansky and Hadas Okon-Singer
Date: Dec 14, 2022，Views：1314
Original protocol used in research article：
Identifying Variables That Predict Depression Following the General Lockdown During the COVID-19 Pandemic
Frontiers in Psychology, May 17, 2021; DOI: 10.3389/fpsyg.2021.680768
Prediction of Mtb-secreted eukaryotic-like proteins
Authors: Qiyao Chai, Zhe Lu, Jing Wang and Cui Hua Liu
Date: Dec 14, 2022，Views：824
Original protocol used in research article：
A bacterial phospholipid phosphatase inhibits host pyroptosis by hijacking ubiquitin
Science, Oct 14, 2022; DOI: 10.1126/science.abq0132
Tissue processing and histological analysis
Author: Jian Yu
Date: Dec 13, 2022，Views：873
Original protocol used in research article：
Interferon b drives intestinal regeneration after radiation
Science Advances, Oct 6, 2021; DOI: 10.1126/sciadv.abi5253
Fibrin clot formation and external fibrinolysis
Authors: Helen R McPherson and Robert AS Ariëns
Date: Dec 13, 2022，Views：862
Original protocol used in research article：
Fibrinogen αC-subregions critically contribute blood clot fibre growth, mechanical stability, and resistance to fibrinolysis
eLife, Oct 11, 2021; DOI: 10.7554/eLife.68761
Flow cytometric analysis to detect KIR+CD8+ T cells and KIR subtypes in human PBMCs
Authors: Jing Li, Naresha Saligrama and Mark M. Davis
Date: Dec 12, 2022，Views：962
Original protocol used in research article：
KIR+CD8+ T cells suppress pathogenic T cells and are active in autoimmune diseases and COVID-19
Science, Mar 8, 2022; DOI: 10.1126/science.abi9591
Site-directed mutagenesis, cell culture and transfection
Author: Da-Wei Zhang
Date: Dec 12, 2022，Views：870
Original protocol used in research article：
Membrane type 1 matrix metalloproteinase promotes LDL receptor shedding and accelerates the development of atherosclerosis
Nature Communications, Mar 25, 2021; DOI: 10.1038/s41467-021-22167-3
IL-1β and IL-18 ELISA
Authors: Kai Sun, Gabriel Mbalaviele and Chun Wang
Date: Dec 12, 2022，Views：1005
Original protocol used in research article：
Fracture healing is delayed in the absence of gasdermin-interleukin-1 signaling
eLife, Mar 4, 2022; DOI: 10.7554/eLife.75753
2.6. Bacterial Zeta Potential
Authors: Karina Arellano-Ayala, Juhwan Lim and Wilhelm-Heinrich Holzapfel
Date: Dec 9, 2022，Views：909
Original protocol used in research article：
Rehydration before Application Improves Functional Properties of Lyophilized Lactiplantibacillus plantarum HAC03
Microorganisms, May 8, 2021; DOI: 10.3390/microorganisms9051013
2.3.3. Caregiver’s Feeding Styles Questionnaire (CFSQ)
Authors: Thomas G. Power, Jennifer O. Fisher, Teresia M. O’Connor, Nilda Micheli, Maria A. Papaioannou and Sheryl O. Hughes
Date: Dec 2, 2022，Views：1288
Original protocol used in research article：
General Parenting and Hispanic Mothers’ Feeding Practices and Styles
International Journal of Environmental Research and Public Health, Jan 6, 2021; DOI: 10.3390/ijerph18020380
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