Protocols shared by authors via RaP service
Journal
Western blotting analysis
Authors: Joseph Wayne M Fowler and William C Sessa
Date: Mar 9, 2023，Views：424
Original protocol used in research article：
Inflammatory stress signaling via NF-kB alters accessible cholesterol to upregulate SREBP2 transcriptional activity in endothelial cells
eLife, Aug 12, 2022; DOI: 10.7554/eLife.79529
Lentivirus infection of organoids for inducible knockdown and activation
Author: Emma L Rawlins
Date: Mar 8, 2023，Views：1272
Original protocol used in research article：
A functional genetic toolbox for human tissue-derived organoids
eLife, Oct 6, 2021; DOI: 10.7554/eLife.67886
Human specimens and cells
Authors: Liam J. O’Neil, Mariana J. Kaplan and Carmelo Carmona-Rivera
Date: Mar 7, 2023，Views：367
Original protocol used in research article：
Neutrophil-mediated carbamylation promotes articular damage in rheumatoid arthritis
Science Advances, Oct 27, 2020; DOI: 10.1126/sciadv.abd2688
Myotube-to-macrophage conditioned medium
Authors: Nicolas J. Pillon and Juleen R. Zierath
Date: Mar 7, 2023，Views：370
Original protocol used in research article：
Distinctive exercise-induced inflammatory response and exerkine induction in skeletal muscle of people with type 2 diabetes
Science Advances, Sep 7, 2022; DOI: 10.1126/sciadv.abo3192
DNA pull-down assays
Author: Matthew W Parker
Date: Mar 6, 2023，Views：516
Original protocol used in research article：
A new class of disordered elements controls DNA replication through initiator self-assembly
eLife, Sep 27, 2019; DOI: 10.7554/eLife.48562
LipidTOX staining
Authors: Meredith H Wilson and Steven A Farber
Date: Mar 6, 2023，Views：554
Original protocol used in research article：
Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish
eLife, Aug 13, 2021; DOI: 10.7554/eLife.66393
Hepatic triglyceride content
Authors: Eric Chang, K. Singer and Mita Varghese
Date: Mar 6, 2023，Views：378
Original protocol used in research article：
Programming effects of maternal and gestational obesity on offspring metabolism and metabolic inflammation
Scientific Reports, Nov 5, 2019; DOI: 10.1038/s41598-019-52583-x
DNA fiber assays
Authors: Sharon B. Cantor and Sumeet Nayak
Date: Mar 4, 2023，Views：507
Original protocol used in research article：
Inhibition of the translesion synthesis polymerase REV1 exploits replication gaps as a cancer vulnerability
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aaz7808
Preparation of fibrin gel components
Authors: Ugur Uslu and Carl H. June
Date: Mar 4, 2023，Views：456
Original protocol used in research article：
Chimeric antigen receptor T cells as adjuvant therapy for unresectable adenocarcinoma
Science Advances, Jan 11, 2023; DOI: 10.1126/sciadv.ade2526
Candidate-gene association mapping
Authors: Xuan Zhang, Jiansheng Li, Jianbing Yan and Xiaohong Yang
Date: Mar 2, 2023，Views：402
Original protocol used in research article：
Convergent selection of a WD40 protein that enhances grain yield in maize and rice
Science, Mar 25, 2022; DOI: 10.1126/science.abg7985
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