Bio-101 - Improve Research Reproducibility

DRG cultures

Authors: Roman J Giger and Ashley L Kalinski
Date: May 4, 2023，Views：664

Original protocol used in research article：

Analysis of the immune response to sciatic nerve injury identifies efferocytosis as a key mechanism of nerve debridement
eLife, Dec 2, 2020; DOI: 10.7554/eLife.60223

Cell culture

Authors: Lingjun Tong, Huaxi Yi and Jiong-Wei Wang
Date: May 3, 2023，Views：761

Original protocol used in research article：

Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis
Science Advances, Apr 12, 2023; DOI: 10.1126/sciadv.ade5041

Code availability

Authors: Chuan Xue and Keith J. Gooch
Date: May 3, 2023，Views：510

Original protocol used in research article：

A Mechanistic Motor-Clutch Model That Explains Cell Shape Dynamics to Cyclic Stretch
Molecular Biology of the Cell, Jan 12, 2022; DOI: 10.1091/mbc.E20-01-0087

Tissue processing for single-cell isolation

Authors: Ana J Caetano and Paul Sharpe
Date: May 3, 2023，Views：545

Original protocol used in research article：

Defining human mesenchymal and epithelial heterogeneity in response to oral inflammatory disease
eLife, Jan 4, 2021; DOI: 10.7554/eLife.62810

Yeast agglutination assay

Authors: Sobieszczańska Beata and Dzięgiel Piotr
Date: May 2, 2023，Views：1041

Original protocol used in research article：

Norepinephrine affects the interaction of adherent-invasive Escherichia coli with intestinal epithelial cells
Virulence, Feb 4, 2021; DOI: 10.1080/21505594.2021.1882780

Preparation of monolayers before mechanical strain

Author: Blair W Benham-Pyle
Date: May 1, 2023，Views：497

Original protocol used in research article：

Increasing β-catenin/Wnt3A activity levels drive mechanical strain-induced cell cycle progression through mitosis
eLife, Oct 26, 2016; DOI: 10.7554/eLife.19799

Fatty acid preparation

Authors: Sathish Kumar Natarajan and Ann Anderson-Berry
Date: May 1, 2023，Views：510

Original protocol used in research article：

Saturated free fatty acids induce placental trophoblast lipoapoptosis
PLoS ONE, Apr 22, 2021; DOI: 10.1371/journal.pone.0249907

Preparation of recombinant ITs

Author: Vidyalakshmi Chandramohan
Date: Apr 29, 2023，Views：439

Original protocol used in research article：

Immunotoxin-αCD40 therapy activates innate and adaptive immunity and generates a durable antitumor response in glioblastoma models
Science Translational Medicine, Feb 8, 2023; DOI: 10.1126/scitranslmed.abn5649

Transfection of miRNA Mimic or Inhibitor

Authors: Si Wang, Jing Xu, Yuanxu Guo, Yongsong Cai, Wenhua Zhu, Liesu Meng, Congshan Jiang and Shemin Lu
Date: Apr 29, 2023，Views：641

Original protocol used in research article：

MicroRNA-497 Reduction and Increase of Its Family Member MicroRNA-424 Lead to Dysregulation of Multiple Inflammation Related Genes in Synovial Fibroblasts With Rheumatoid Arthritis
Frontiers in Immunology, Mar 26, 2021; DOI: 10.3389/fimmu.2021.619392

Generation of transgenic Xist-BglG Halo ES cell line

Authors: Heather Coker, Lothar Schermelleh and Neil Brockdorff
Date: Apr 28, 2023，Views：444

Original protocol used in research article：

Time-resolved structured illumination microscopy reveals key principles of Xist RNA spreading
Science, Jun 11, 2021; DOI: 10.1126/science.abe7500

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