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A high-quality database for basic life science protocols
Protocols
Research fields
Biochemistry (1376)
Biophysics (164)
Cancer Biology (431)
Cell Biology (2122)
Developmental Biology (321)
Immunology (597)
Microbiology (1154)
Molecular Biology (1132)
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Stem Cell (252)
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Fatty acid preparation
Authors:
Sathish Kumar Natarajan
and
Ann Anderson-Berry
Date: May 1, 2023,Views:508
Original protocol used in research article:
Saturated free fatty acids induce placental trophoblast lipoapoptosis
PLoS ONE, Apr 22, 2021; DOI:
10.1371/journal.pone.0249907
Preparation of recombinant ITs
Author:
Vidyalakshmi Chandramohan
Date: Apr 29, 2023,Views:438
Original protocol used in research article:
Immunotoxin-αCD40 therapy activates innate and adaptive immunity and generates a durable antitumor response in glioblastoma models
Science Translational Medicine, Feb 8, 2023; DOI:
10.1126/scitranslmed.abn5649
Transfection of miRNA Mimic or Inhibitor
Authors:
Si Wang
,
Jing Xu
,
Yuanxu Guo
,
Yongsong Cai
,
Wenhua Zhu
,
Liesu Meng
,
Congshan Jiang
and
Shemin Lu
Date: Apr 29, 2023,Views:609
Original protocol used in research article:
MicroRNA-497 Reduction and Increase of Its Family Member MicroRNA-424 Lead to Dysregulation of Multiple Inflammation Related Genes in Synovial Fibroblasts With Rheumatoid Arthritis
Frontiers in Immunology, Mar 26, 2021; DOI:
10.3389/fimmu.2021.619392
Generation of transgenic Xist-BglG Halo ES cell line
Authors:
Heather Coker
,
Lothar Schermelleh
and
Neil Brockdorff
Date: Apr 28, 2023,Views:444
Original protocol used in research article:
Time-resolved structured illumination microscopy reveals key principles of Xist RNA spreading
Science, Jun 11, 2021; DOI:
10.1126/science.abe7500
Purification and treatment of recombinant human RyR2
Authors:
Marco C. Miotto
and
Andrew R. Marks
Date: Apr 27, 2023,Views:428
Original protocol used in research article:
Structural analyses of human ryanodine receptor type 2 channels reveal the mechanisms for sudden cardiac death and treatment
Science Advances, Jul 20, 2022; DOI:
10.1126/sciadv.abo1272
DNA fibre assay
Authors:
Kasper Fugger
and
Stephen C. West
Date: Apr 27, 2023,Views:436
Original protocol used in research article:
Targeting the nucleotide salvage factor DNPH1 sensitizes BRCA-deficient cells to PARP inhibitors
Science, Apr 8, 2021; DOI:
10.1126/science.abb4542
4.8.1. BSA Denaturation InhibitionAssay
Authors:
Rajlakshmi Devi
and
Devasish Chowdhury
Date: Apr 26, 2023,Views:1167
Original protocol used in research article:
Green Synthesis of Gold Nano-bioconjugates from Onion Peel Extract and Evaluation of Their Antioxidant, Anti-inflammatory, and Cytotoxic Studies
ACS Omega, Jul 8, 2021; DOI:
10.1021/acsomega.1c00861
Induction of recombination by 4-OH Tamoxifen intraperitoneal injection in adult zebrafish
Authors:
Ivonne Sehring
and
Gilbert Weidinger
Date: Apr 25, 2023,Views:769
Original protocol used in research article:
Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses
eLife, ; DOI:
10.7554/eLife.77614
Pteridine autofluorescence
Authors:
Delai Huang
and
David M Parichy
Date: Apr 24, 2023,Views:393
Original protocol used in research article:
Development and genetics of red coloration in the zebrafish relative
Danio albolineatus
eLife, Aug 26, 2021; DOI:
10.7554/eLife.70253
Gene set enrichment analysis
Authors:
Vahab D Soleimani
and
Korin Sahinyan
Date: Apr 24, 2023,Views:409
Original protocol used in research article:
Application of ATAC-Seq for genome-wide analysis of the chromatin state at single myofiber resolution
eLife, Feb 21, 2022; DOI:
10.7554/eLife.72792
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