Protocols shared by authors via RaP service
Journal
High-resolution and deep phylogenetic reconstruction of ancestral states from large transcriptomic data sets
Author: Sumanth Mutte
Date: Nov 27, 2019，Views：1291
Original protocol used in research article：
Origin and evolution of the nuclear auxin response system
eLife, Mar 27, 2018; DOI: 10.7554/eLife.33399
GFP-based Luria-Delbrück fluctuation test
Author: Adam S Lauring
Date: Nov 27, 2019，Views：1458
Original protocol used in research article：
A novel twelve class fluctuation test reveals higher than expected mutation rates for influenza A viruses
eLife, Jun 9, 2017; DOI: 10.7554/eLife.26437
Plasmids generation
Author: Harihar Basnet
Date: Nov 24, 2019，Views：1116
Original protocol used in research article：
Flura-seq identifies organ-specific metabolic adaptations during early metastatic colonization
eLife, Mar 26, 2019; DOI: 10.7554/eLife.43627
CRISPR/Cas9 genome editing
Author: Morayma Temoche
Date: Nov 23, 2019，Views：1589
Original protocol used in research article：
Distinct mechanisms of microRNA sorting into cancer cell-derived extracellular vesicle subtypes
eLife, Aug 22, 2019; DOI: 10.7554/eLife.47544
Fly stocks and genetics
Author: Helmut Krämer
Date: Nov 23, 2019，Views：1265
Original protocol used in research article：
Adaptation to constant light requires Fic-mediated AMPylation of BiP to protect against reversible photoreceptor degeneration
eLife, Jul 17, 2018; DOI: 10.7554/eLife.38752
SIM imaging
Author: Sue L Jaspersen
Date: Nov 21, 2019，Views：1345
Original protocol used in research article：
Structured illumination with particle averaging reveals novel roles for yeast centrosome components during duplication
eLife, Sep 15, 2015; DOI: 10.7554/eLife.08586
Behavior
Author: Michael J Goard
Date: Nov 21, 2019，Views：1256
Original protocol used in research article：
Distinct roles of visual, parietal, and frontal motor cortices in memory-guided sensorimotor decisions
eLife, Aug 4, 2016; DOI: 10.7554/eLife.13764
ESC-derived motor neurons
Author: Esteban O. Mazzoni
Date: Nov 21, 2019，Views：1221
Original protocol used in research article：
Stem cell-derived cranial and spinal motor neurons reveal proteostatic differences between ALS resistant and sensitive motor neurons
eLife, Jun 3, 2019; DOI: 10.7554/eLife.44423
Influenza A genome sequencing
Author: Nacho Mena
Date: Nov 20, 2019，Views：1676
Original protocol used in research article：
Origins of the 2009 H1N1 influenza pandemic in swine in Mexico
eLife, Jun 28, 2016; DOI: 10.7554/eLife.16777
Desiccation survival
Author: Jennifer Sun
Date: Nov 18, 2019，Views：1072
Original protocol used in research article：
Humidity response depends on the small soluble protein Obp59a in Drosophila
eLife, Sep 19, 2018; DOI: 10.7554/eLife.39249
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