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Development of the aspiration-assisted bioprinter
Last updated date: Mar 25, 2020 Views: 955 Forks: 0
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Detailed information for construction of the aspiration-assisted bioprinter can be found in the supplementary information of the published paper.
For the second question, here are the steps:
- Spheroids were collected into a 1.5 ml conical tube from 96-well plate.
- The conical tube was transfer to the bioprinting platform, and removed the top of the tube by a scissors under sterile condition.
- The conical tube was then mounted on a 3D printed holder which helps to hold the tube straight.
- A customized glass pipette with ~80-micron diameter dipped at the bottom of the tube, and we apply 25 mmHg air for a second in order to levitate all spheroid.
- Then minimum lifting pressure was applied in order to pick and lift a spheroid into air.
- This was repeated as many times as needed.
- Ozbolat, I(2020). Development of the aspiration-assisted bioprinter. Bio-protocol Preprint. bio-protocol.org/prep255.
- Ayan, B., Heo, D. N., Zhang, Z., Dey, M., Povilianskas, A., Drapaca, C. and Ozbolat, I. T.(2020). Aspiration-assisted bioprinting for precise positioning of biologics . Science Advances 6(10). DOI: 10.1126/sciadv.aaw5111
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