Protocols shared by authors via RaP service
Journal
Purification of mammalian dynein-dynactin
Author: Ahmet Yildiz
Date: Dec 3, 2019，Views：1193
Original protocol used in research article：
Kinesin and dynein use distinct mechanisms to bypass obstacles
eLife, Sep 9, 2019; DOI: 10.7554/eLife.48629
In vitro differentiation of mESCs in embryoid bodies
Author: Lars Schomacher
Date: Dec 3, 2019，Views：1236
Original protocol used in research article：
NEIL1 and NEIL2 DNA glycosylases protect neural crest development against mitochondrial oxidative stress
eLife, Sep 30, 2019; DOI: 10.7554/eLife.49044
Platelet isolation and quantification
Author: Mercedes Costell
Date: Dec 1, 2019，Views：1193
Original protocol used in research article：
The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes
eLife, Jan 16, 2017; DOI: 10.7554/eLife.22264
Preparation of mini-Gs heterotrimer
Author: Christopher Gordon Tate
Date: Dec 1, 2019，Views：1748
Original protocol used in research article：
Cryo-EM structure of the adenosine A2A receptor coupled to an engineered heterotrimeric G protein
eLife, May 4, 2018; DOI: 10.7554/eLife.35946
High-resolution and deep phylogenetic reconstruction of ancestral states from large transcriptomic data sets
Author: Sumanth Mutte
Date: Nov 27, 2019，Views：1363
Original protocol used in research article：
Origin and evolution of the nuclear auxin response system
eLife, Mar 27, 2018; DOI: 10.7554/eLife.33399
GFP-based Luria-Delbrück fluctuation test
Author: Adam S Lauring
Date: Nov 27, 2019，Views：1547
Original protocol used in research article：
A novel twelve class fluctuation test reveals higher than expected mutation rates for influenza A viruses
eLife, Jun 9, 2017; DOI: 10.7554/eLife.26437
Plasmids generation
Author: Harihar Basnet
Date: Nov 24, 2019，Views：1163
Original protocol used in research article：
Flura-seq identifies organ-specific metabolic adaptations during early metastatic colonization
eLife, Mar 26, 2019; DOI: 10.7554/eLife.43627
CRISPR/Cas9 genome editing
Author: Morayma Temoche
Date: Nov 23, 2019，Views：1648
Original protocol used in research article：
Distinct mechanisms of microRNA sorting into cancer cell-derived extracellular vesicle subtypes
eLife, Aug 22, 2019; DOI: 10.7554/eLife.47544
Fly stocks and genetics
Author: Helmut Krämer
Date: Nov 23, 2019，Views：1318
Original protocol used in research article：
Adaptation to constant light requires Fic-mediated AMPylation of BiP to protect against reversible photoreceptor degeneration
eLife, Jul 17, 2018; DOI: 10.7554/eLife.38752
SIM imaging
Author: Sue L Jaspersen
Date: Nov 21, 2019，Views：1398
Original protocol used in research article：
Structured illumination with particle averaging reveals novel roles for yeast centrosome components during duplication
eLife, Sep 15, 2015; DOI: 10.7554/eLife.08586
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