Protocols shared by authors via RaP service
Journal
Metabolite extraction and LC-MS analysis
Author: Eugene I Shakhnovich
Date: Dec 12, 2019，Views：1175
Original protocol used in research article：
Adaptation to mutational inactivation of an essential gene converges to an accessible suboptimal fitness peak
eLife, Oct 1, 2019; DOI: 10.7554/eLife.50509
ChIP-Sequencing
Author: Neal A Deluca
Date: Dec 12, 2019，Views：1537
Original protocol used in research article：
Herpes simplex viral nucleoprotein creates a competitive transcriptional environment facilitating robust viral transcription and host shut off
eLife, Oct 22, 2019; DOI: 10.7554/eLife.51109
Four-quadrant behavioral assay
Authors: Christopher J Potter and Chun-Chieh Lin
Date: Dec 12, 2019，Views：1217
Original protocol used in research article：
Food odors trigger Drosophila males to deposit a pheromone that guides aggregation and female oviposition decisions
eLife, Sep 30, 2015; DOI: 10.7554/eLife.08688
In silico analyses of SecA pore dynamics
Author: Ian Collinson
Date: Dec 12, 2019，Views：1135
Original protocol used in research article：
HDX-MS reveals nucleotide-dependent, anti-correlated opening and closure of SecA and SecY channels of the bacterial translocon
eLife, Jul 10, 2019; DOI: 10.7554/eLife.47402
In situ hybridization protocol
Author: Peng Huang
Date: Dec 11, 2019，Views：1090
Original protocol used in research article：
Notch signalling maintains Hedgehog responsiveness via a Gli-dependent mechanism during spinal cord patterning in zebrafish
eLife, Aug 27, 2019; DOI: 10.7554/eLife.49252
3D reconstruction (and others)
Author: David M. Miller
Date: Dec 11, 2019，Views：1121
Original protocol used in research article：
C. elegans neurons have functional dendritic spines
eLife, Oct 4, 2019; DOI: 10.7554/eLife.47918
Genotyping
Author: Cunming Duan
Date: Dec 11, 2019，Views：1203
Original protocol used in research article：
Cell-autonomous regulation of epithelial cell quiescence by calcium channel Trpv6
eLife, Sep 17, 2019; DOI: 10.7554/eLife.48003
Analysis of spatial scale sizes
Author: Michael Peer
Date: Dec 11, 2019，Views：1093
Original protocol used in research article：
Processing of different spatial scales in the human brain
eLife, Sep 10, 2019; DOI: 10.7554/eLife.47492
Cuticle preparations
Authors: Yoseop Yoon and Urs Schmidt-Ott
Date: Dec 11, 2019，Views：1377
Original protocol used in research article：
Embryo polarity in moth flies and mosquitoes relies on distinct old genes with localized transcript isoforms
eLife, Oct 8, 2019; DOI: 10.7554/eLife.46711
Generation of HeLa KO cells with CRISPR
Authors: Florian Ullrich and Thomas J Jentsch
Date: Dec 11, 2019，Views：1239
Original protocol used in research article：
Identification of TMEM206 proteins as pore of PAORAC/ASOR acid-sensitive chloride channels
eLife, Jul 18, 2019; DOI: 10.7554/eLife.49187
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