Protocols shared by authors via RaP service
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Generation of HeLa KO cells with CRISPR
Authors: Florian Ullrich and Thomas J Jentsch
Date: Dec 11, 2019，Views：1239
Original protocol used in research article：
Identification of TMEM206 proteins as pore of PAORAC/ASOR acid-sensitive chloride channels
eLife, Jul 18, 2019; DOI: 10.7554/eLife.49187
Imaging Orc1 cellular dynamics in Drosophila S2 cells
Author: Michael R Botchan
Date: Dec 10, 2019，Views：1107
Original protocol used in research article：
A new class of disordered elements controls DNA replication through initiator self-assembly
eLife, Sep 27, 2019; DOI: 10.7554/eLife.48562
Large-scale metabolome analysis
Authors: Pamela Maher and Antonio Currais
Date: Dec 9, 2019，Views：1527
Original protocol used in research article：
Elevating acetyl-CoA levels reduces aspects of brain aging
eLife, Nov 19, 2019; DOI: 10.7554/eLife.47866
Respiration measurements
Author: David C Chan
Date: Dec 8, 2019，Views：1182
Original protocol used in research article：
Mitochondrial fusion is required for spermatogonial differentiation and meiosis
eLife, Oct 9, 2019; DOI: 10.7554/eLife.51601
Drosha cleavage assay
Author: Tsui Han Loo
Date: Dec 6, 2019，Views：1227
Original protocol used in research article：
The mammalian LINC complex component SUN1 regulates muscle regeneration by modulating drosha activity
eLife, Nov 5, 2019; DOI: 10.7554/eLife.49485
Immunofluorescence staining - Vibratome Sections for Oligodendrocyte morphological analysis
Author: Matthew Swire
Date: Dec 6, 2019，Views：10195
Original protocol used in research article：
Endothelin signalling mediates experience-dependent myelination in the CNS
eLife, Oct 28, 2019; DOI: 10.7554/eLife.49493
General Protocol for Resistance Assays in Liquid Media
Author: Nathaniel J Traaseth
Date: Dec 4, 2019，Views：1141
Original protocol used in research article：
Inducing conformational preference of the membrane protein transporter EmrE through conservative mutations
eLife, Oct 22, 2019; DOI: 10.7554/eLife.48909
C2C12 culture and generation of Gene-modified C2C12 cells
Author: Lin Mei
Date: Dec 4, 2019，Views：1226
Original protocol used in research article：
A mechanism in agrin signaling revealed by a prevalent Rapsyn mutation in congenital myasthenic syndrome
eLife, Sep 24, 2019; DOI: 10.7554/eLife.49180
Quantification of tracheal density in IFMs
Author: Stefan Luschnig
Date: Dec 4, 2019，Views：1200
Original protocol used in research article：
Matrix metalloproteinase 1 modulates invasive behavior of tracheal branches during entry into Drosophila flight muscles
eLife, Oct 2, 2019; DOI: 10.7554/eLife.48857
Growth chamber experiments
Author: Steven Penfield
Date: Dec 4, 2019，Views：1198
Original protocol used in research article：
Flowering time and seed dormancy control use external coincidence to generate life history strategy
eLife, Mar 31, 2015; DOI: 10.7554/eLife.05557
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