Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:

Kather, J(2020). Automatic determination of optimal cluster number. Bio-protocol Preprint. bio-protocol.org/prep219.
Kather, J. N., Suarez-Carmona, M., Charoentong, P., Weis, C., Hirsch, D., Bankhead, P., Horning, M., Ferber, D., Kel, I., Herpel, E., Schott, S., Zörnig, I., Utikal, J., Marx, A., Gaiser, T., Brenner, H., Chang-Claude, J., Hoffmeister, M., Jäger, D. and Halama, N.(2018). Topography of cancer-associated immune cells in human solid tumors. eLIFE. DOI: 10.7554/eLife.36967