Bio-101 - Improve Research Reproducibility

Pfs25 and PfMGET RNA quantification

Authors: Isaie J Reuling and Teun Bousema
Date: Sep 29, 2023，Views：223

Original protocol used in research article：

A randomized feasibility trial comparing four antimalarial drug regimens to induce Plasmodium falciparum gametocytemia in the controlled human malaria infection model
eLife, Feb 27, 2018; DOI: 10.7554/eLife.31549

CODEX antibody validation, titration, and staining

Author: Christian M. Schürch
Date: Sep 27, 2023，Views：16

Original protocol used in research article：

A tissue atlas of ulcerative colitis revealing evidence of sex-dependent differences in disease-driving inflammatory cell types and resistance to TNF inhibitor therapy
Science Advances, Jan 20, 2023; DOI: 10.1126/sciadv.add1166

CFSE-MLR and cell sorting protocol to define alloreactive T cell repertoires by high throughput TCR sequencing

Authors: Jianing Fu and Megan Sykes
Date: Sep 26, 2023，Views：79

Original protocol used in research article：

Lymphohematopoietic graft-versus-host responses promote mixed chimerism in patients receiving intestinal transplantation
The Journal of Clinical Investigation, Apr 15, 2021; DOI: 10.1172/jci141698

Viral copy number

Authors: Paula Rodriguez-Marquez, Juan R. Rodriguez-Madoz and Felipe Prosper
Date: Sep 26, 2023，Views：27

Original protocol used in research article：

CAR density influences antitumoral efficacy of BCMA CAR T cells and correlates with clinical outcome
Science Advances, Sep 30, 2022; DOI: 10.1126/sciadv.abo0514

Automated Layer Analysis (ALAn): An Image Analysis Tool for the Unbiased Characterization of Mammalian Epithelial Architecture in Culture

Authors: Christian Cammarota, Dan T Bergstralh and Tara M Finegan
Date: Sep 25, 2023，Views：204

Original protocol used in research article：

Neuroinflammation inhibition by small-molecule targeting USP7 noncatalytic domain for neurodegenerative disease therapy
Science Advances, Aug 10, 2022; DOI: 10.1126/sciadv.abo0789

Generation of single cell suspensions of Mtb

Authors: Ekansh Mittal and Jennifer A Philips
Date: Sep 25, 2023，Views：120

Original protocol used in research article：

Single cell preparations of Mycobacterium tuberculosis damage the mycobacterial envelope and disrupt macrophage interactions
eLife, Feb 28, 2023; DOI: 10.7554/eLife.85416

m6A-RIP-qPCR

Authors: Lilin Ye, Yun-Gui Yang and Shuyang Yu
Date: Sep 24, 2023，Views：166

Original protocol used in research article：

METTL3-dependent m6A modification programs T follicular helper cell differentiation
Nature Communications, Feb 26, 2021; DOI: 10.1038/s41467-021-21594-6

Drosophila Testis FISH

Authors: Amelie A Raz, Stephen DiNardo and Margaret T Fuller
Date: Sep 22, 2023，Views：53

Original protocol used in research article：

Emergent dynamics of adult stem cell lineages from single nucleus and single cell RNA-Seq of Drosophila testes
eLife, Feb 16, 2023; DOI: 10.7554/eLife.82201

Ex vivo digestion of B-mEVs, H-mEVs, and liposomes

Authors: Lingjun Tong, Sitong Zhang, Huaxi Yi and Jiong-Wei Wang
Date: Sep 20, 2023，Views：65

Original protocol used in research article：

Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis
Science Advances, Apr 12, 2023; DOI: 10.1126/sciadv.ade5041

ELISA for TGFB1 quantification

Authors: Deepak A Kaji and Alice H Huang
Date: Sep 17, 2023，Views：46

Original protocol used in research article：

Tgfβ signaling is required for tenocyte recruitment and functional neonatal tendon regeneration
eLife, Jun 5, 2020; DOI: 10.7554/eLife.51779

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