Protocols shared by authors via RaP service
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Fluorescence activated cell sorting in Schistosoma mansoni
Author: George R Wendt
Date: Apr 28, 2020，Views：1105
Original protocol used in research article：
Flatworm-specific transcriptional regulators promote the specification of tegumental progenitors in Schistosoma mansoni
eLife, Mar 20, 2018; DOI: 10.7554/eLife.33221
Bioinformatic analysis of the ChIP-seq and CUT&RUN datasets
Authors: Huacheng Luo and Jianrong Lu
Date: Apr 25, 2020，Views：1495
Original protocol used in research article：
LATS kinase–mediated CTCF phosphorylation and selective loss of genomic binding
Science Advances, Feb 19, 2020; DOI: 10.1126/sciadv.aaw4651
Chromatin immunoprecipitation (ChIP) assay
Authors: Shuchi Agrawal Singh and Klaus Hansen
Date: Apr 24, 2020，Views：2558
Original protocol used in research article：
PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells
eLife, Jan 23, 2019; DOI: 10.7554/eLife.40364
Preparation and use of stimulation beads
Authors: Michael C. Milone and Benjamin I Philipson
Date: Apr 23, 2020，Views：1063
Original protocol used in research article：
4-1BB costimulation promotes CAR T cell survival through noncanonical NF-κB signaling
Science Signaling, Mar 30, 2020; DOI: 10.1126/scisignal.aay8248
Protein expression
Author: Caileen M Brison
Date: Apr 22, 2020，Views：1177
Original protocol used in research article：
An order-to-disorder structural switch activates the FoxM1 transcription factor
eLife, May 28, 2019; DOI: 10.7554/eLife.46131
Aversive phototaxic suppression (APS)
Authors: Chunghun Lim and Yoonhee Ki
Date: Apr 21, 2020，Views：1688
Original protocol used in research article：
Sleep-promoting effects of threonine link amino acid metabolism in Drosophila neuron to GABAergic control of sleep drive
eLife, Jul 17, 2019; DOI: 10.7554/eLife.40593
Generation and LED light photoactivation of Opto-receptors
Authors: Anassuya Ramachandran and Caroline S Hill
Date: Apr 20, 2020，Views：1113
Original protocol used in research article：
TGF-β uses a novel mode of receptor activation to phosphorylate SMAD1/5 and induce epithelial-to-mesenchymal transition
eLife, Jan 29, 2018; DOI: 10.7554/eLife.31756
Magnet-assisted flow cytometry of in vivo tumors to quantitate cell-specific responses to magnetic iron oxide nanoparticles
Authors: Preethi Korangath and Robert Ivkov
Date: Apr 17, 2020，Views：1072
Original protocol used in research article：
Nanoparticle interactions with immune cells dominate tumor retention and induce T cell–mediated tumor suppression in models of breast cancer
Science Advances, Mar 24, 2020; DOI: 10.1126/sciadv.aay1601
RNA seq data analysis
Author: Ilaria Granata
Date: Apr 15, 2020，Views：1089
Original protocol used in research article：
Human axial progenitors generate trunk neural crest cells in vitro
eLife, Aug 10, 2018; DOI: 10.7554/eLife.35786
Immunofluorescent staining of mouse embryonic tissues
Authors: Jingyue Xu and Rulang Jiang
Date: Apr 14, 2020，Views：970
Original protocol used in research article：
Hedgehog signaling patterns the oral-aboral axis of the mandibular arch
eLife, Jan 14, 2019; DOI: 10.7554/eLife.40315
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