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A high-quality database for basic life science protocols
Protocols
Research fields
Biochemistry (1361)
Biophysics (158)
Cancer Biology (423)
Cell Biology (2085)
Developmental Biology (314)
Immunology (592)
Microbiology (1142)
Molecular Biology (1113)
Neuroscience (686)
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Stem Cell (246)
Systems Biology (330)
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Virulence
3-D printing of window and clip
Authors:
Qiang Huang
,
Rudolf Jaenisch
and
Xiling Shen
Date: May 7, 2021,Views:871
Original protocol used in research article:
Intravital imaging of mouse embryos
Science, Apr 9, 2020; DOI:
10.1126/science.aba0210
Sperm penetration and binding assays
Authors:
Ismael Lamas-Toranzo
,
María Jiménez-Movilla
and
Pablo Bermejo-Álvarez
Date: May 7, 2021,Views:820
Original protocol used in research article:
TMEM95 is a sperm membrane protein essential for mammalian fertilization
eLife, Jun 2, 2020; DOI:
10.7554/eLife.53913
Isolation and phospholipid enrichment of muscle mitochondria and mitoplasts
Authors:
Alexandre Prola
,
Aymeline Vandestienne
,
Frédéric Joubert
,
Laurent Tiret
and
Fanny Pilot-Storck
Date: May 7, 2021,Views:1110
Original protocol used in research article:
Cardiolipin content controls mitochondrial coupling and energetic efficiency in muscle
Science Advances, Dec 31, 2020; DOI:
10.1126/sciadv.abd6322
FUCCI live-cell imaging
Author:
Andrew Burgess
Date: May 6, 2021,Views:904
Original protocol used in research article:
Analysis of pulsed cisplatin signalling dynamics identifies effectors of resistance in lung adenocarcinoma
eLife, Jun 9, 2020; DOI:
10.7554/eLife.53367
PhosFate analysis
Authors:
Jonathan M Budzik
and
Jeffery S Cox
Date: May 6, 2021,Views:883
Original protocol used in research article:
Dynamic post-translational modification profiling of
Mycobacterium tuberculosis
-infected primary macrophages
eLife, Jan 17, 2020; DOI:
10.7554/eLife.51461
Web app
Authors:
Jonathan M Budzik
and
Jeffery S Cox
Date: May 6, 2021,Views:886
Original protocol used in research article:
Dynamic post-translational modification profiling of
Mycobacterium tuberculosis
-infected primary macrophages
eLife, Jan 17, 2020; DOI:
10.7554/eLife.51461
RNAseq
Authors:
Jonathan M Budzik
and
Jeffery S Cox
Date: May 6, 2021,Views:805
Original protocol used in research article:
Dynamic post-translational modification profiling of
Mycobacterium tuberculosis
-infected primary macrophages
eLife, Jan 17, 2020; DOI:
10.7554/eLife.51461
Immunofluorescence microscopy for LC3
Authors:
Jonathan M Budzik
and
Jeffery S Cox
Date: May 6, 2021,Views:923
Original protocol used in research article:
Dynamic post-translational modification profiling of
Mycobacterium tuberculosis
-infected primary macrophages
eLife, Jan 17, 2020; DOI:
10.7554/eLife.51461
ATAC-seq
Authors:
Heinrich Jasper
and
Helen Marie Tauc
Date: May 6, 2021,Views:910
Original protocol used in research article:
Age-related changes in polycomb gene regulation disrupt lineage fidelity in intestinal stem cells
eLife, Mar 16, 2021; DOI:
10.7554/eLife.62250
Post-translational histone modifications panel (active motif)
Authors:
Heinrich Jasper
and
Helen Marie Tauc
Date: May 6, 2021,Views:830
Original protocol used in research article:
Age-related changes in polycomb gene regulation disrupt lineage fidelity in intestinal stem cells
eLife, Mar 16, 2021; DOI:
10.7554/eLife.62250
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