Protocols shared by authors via RaP service
Journal
co-immunoprecipitation and immunoblotting
Authors: Taifeng Zhou, Peiqiang Su and Yingzi Yang
Date: May 12, 2021，Views：2466
Original protocol used in research article：
Piezo1/2 mediate mechanotransduction essential for bone formation through concerted activation of NFAT-YAP1-ß-catenin
eLife, Mar 18, 2020; DOI: 10.7554/eLife.52779
T cell isolation
Authors: Scott B Thompson and Jordan Jacobelli
Date: May 11, 2021，Views：1126
Original protocol used in research article：
Formin-like 1 mediates effector T cell trafficking to inflammatory sites to enable T cell-mediated autoimmunity
eLife, Jun 8, 2020; DOI: 10.7554/eLife.58046
Barley spikelet morphometry analysis
Author: Ravi Koppolu
Date: May 11, 2021，Views：854
Original protocol used in research article：
Transcriptional landscapes of floral meristems in barley
Science Advances, Apr 28, 2021; DOI: 10.1126/sciadv.abf0832
Culture of mouse/rat primary neurons
Authors: Richard L Huganir and Huaqiang Fang
Date: May 11, 2021，Views：1219
Original protocol used in research article：
An optimized CRISPR/Cas9 approach for precise genome editing in neurons
eLife, Mar 10, 2021; DOI: 10.7554/eLife.65202
Expression and purification of recombinant FOXO3-DBD
Authors: Judith Hagenbuchner, Tomas Obsil, Michael J Ausserlechner and Veronika Obsilova
Date: May 10, 2021，Views：792
Original protocol used in research article：
Modulating FOXO3 transcriptional activity by small, DBD-binding molecules
eLife, Dec 4, 2019; DOI: 10.7554/eLife.48876
Binning approach and normalization
Authors: Nina Kirstein, Olivier Hyrien, Benjamin Audit and Aloys Schepers
Date: May 10, 2021，Views：864
Original protocol used in research article：
Human ORC/MCM density is low in active genes and correlates with replication time but does not delimit initiation zones
eLife, Mar 8, 2021; DOI: 10.7554/eLife.62161
Protein extraction and immunobloting
Authors: Ismael Lamas-Toranzo, Julieta G Hamze, María Jiménez-Movilla and Pablo Bermejo-Álvarez
Date: May 10, 2021，Views：908
Original protocol used in research article：
TMEM95 is a sperm membrane protein essential for mammalian fertilization
eLife, Jun 2, 2020; DOI: 10.7554/eLife.53913
Immunoblotting
Authors: Benjamin Demarco, Kaiwen Chen and Petr Broz
Date: May 10, 2021，Views：827
Original protocol used in research article：
Caspase-8–dependent gasdermin D cleavage promotes antimicrobial defense but confers susceptibility to TNF-induced lethality
Science Advances, Nov 18, 2020; DOI: 10.1126/sciadv.abc3465
Gene-specific m6A qPCR
Authors: Chris Bass and Youjun Zhang
Date: May 9, 2021，Views：1237
Original protocol used in research article：
Epitranscriptomic regulation of insecticide resistance
Science Advances, May 5, 2021; DOI: 10.1126/sciadv.abe5903
Statistical analysis
Authors: David Chen, Josephine Wu and Gordana Vunjak-Novakovic
Date: May 9, 2021，Views：863
Original protocol used in research article：
Tissue engineered autologous cartilage-bone grafts for temporomandibular joint regeneration
Science Translational Medicine, Oct 13, 2020; DOI: 10.1126/scitranslmed.abb6683
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