Protocols shared by authors via RaP service
Journal
Cell-free degradation assays
Authors: Qingyan Shu and Liangsheng Wang
Date: Jul 7, 2021，Views：1850
Original protocol used in research article：
Chalcone synthase is ubiquitinated and degraded via interactions with a RING-H2 protein in petals of Paeonia ‘He Xie’
Journal of Experimental Botany, May 18, 2019; DOI: 10.1093/jxb/erz245
Labile Zinc Measurement by Flow Cytometry
Authors: Aleksha Panwar and Guruprasad R. Medigeshi
Date: Jul 6, 2021，Views：949
Original protocol used in research article：
Zinc Chelation Specifically Inhibits Early Stages of Dengue Virus Replication by Activation of NF-κB and Induction of Antiviral Response in Epithelial Cells
Frontiers in Immunology, Oct 1, 2019; DOI: 10.3389/fimmu.2019.02347
Immunoassay analysis of poly(GP)
Authors: Nadja S. Andrade and Zane Zeier
Date: Jul 6, 2021，Views：901
Original protocol used in research article：
Dipeptide repeat proteins inhibit homology-directed DNA double strand break repair in C9ORF72 ALS/FTD
Molecular Neurodegeneration, Feb 24, 2020; DOI: 10.1186/s13024-020-00365-9
Mitochondrial pyruvate transport assay
Authors: Robinson Arce-Molina, L Felipe Barros and Alejandro San Martín
Date: Jul 6, 2021，Views：1142
Original protocol used in research article：
A highly responsive pyruvate sensor reveals pathway-regulatory role of the mitochondrial pyruvate carrier MPC
eLife, Mar 6, 2020; DOI: 10.7554/eLife.53917
Sequential CNS population isolations
Authors: Michelle L Olsen and Raymundo D Hernandez
Date: Jul 6, 2021，Views：901
Original protocol used in research article：
Astrocyte morphogenesis is dependent on BDNF signaling via astrocytic TrkB.T1
eLife, Aug 21, 2019; DOI: 10.7554/eLife.44667
2.7. Efferocytosis assay
Author: Vladimir Leksa
Date: Jul 6, 2021，Views：1212
Original protocol used in research article：
The mannose 6‐phosphate/insulin‐like growth factor 2 receptor mediates plasminogen‐induced efferocytosis
Journal of Leukocyte Biology, Jan 18, 2019; DOI: 10.1002/JLB.1AB0417-160RR
Complex preparation and crystallization
Authors: Shuguang Tan, Hui Feng and Jinghua Yan
Date: Jul 5, 2021，Views：894
Original protocol used in research article：
Glycosylation-independent binding of monoclonal antibody toripalimab to FG loop of PD-1 for tumor immune checkpoint therapy
mAbs, Apr 19, 2019; DOI: 10.1080/19420862.2019.1596513
Isolation of stem cells
Author: Anna Halling Folkmar Andersen
Date: Jul 5, 2021，Views：899
Original protocol used in research article：
Comparable human reconstitution following Cesium-137 versus X-ray irradiation preconditioning in immunodeficient NOG mice
PLoS ONE, Oct 29, 2020; DOI: 10.1371/journal.pone.0241375
2.7. Purification of VEEV-RABV-G virions
Authors: Ya-Nan Zhang and Bo Zhang
Date: Jul 4, 2021，Views：944
Original protocol used in research article：
A novel rabies vaccine based on infectious propagating particles derived from hybrid VEEV-Rabies replicon
EBioMedicine, Jun 5, 2020; DOI: 10.1016/j.ebiom.2020.102819
Typhoid toxin disassembly assay
Author: SHU-JUNG CHANG
Date: Jul 4, 2021，Views：904
Original protocol used in research article：
Unique features in the intracellular transport of typhoid toxin revealed by a genome-wide screen
PLoS Pathogens, Apr 5, 2019; DOI: 10.1371/journal.ppat.1007704
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