Protocols shared by authors via RaP service
Journal
Preparation of Aβ solution
Author: Peter Niraj Nirmalraj
Date: Jun 7, 2021，Views：885
Original protocol used in research article：
Complete aggregation pathway of amyloid β (1-40) and (1-42) resolved on an atomically clean interface
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aaz6014
Reverse transcription (RT) and quantitative RT-PCR analysis
Authors: Ziling Fang, Shelya X Zeng, Jianping Xiong and Hua Lu
Date: Jun 6, 2021，Views：843
Original protocol used in research article：
SPIN1 promotes tumorigenesis by blocking the uL18 (universal large ribosomal subunit protein 18)-MDM2-p53 pathway in human cancer
eLife, Mar 16, 2018; DOI: 10.7554/eLife.31275
Parallel reaction monitoring MS
Authors: Eric Chiles, Amanda Williams, Debashish Bhattacharya and Xiaoyang Su
Date: Jun 4, 2021，Views：873
Original protocol used in research article：
Metabolomic shifts associated with heat stress in coral holobionts
Science Advances, Dec 31, 2020; DOI: 10.1126/sciadv.abd4210
Wounding Zebrafish Larval Epidermis by Laceration
Authors: Andrew S Kennard, Ellen Labuz, Christopher K Prinz and Julie A Theriot
Date: Jun 3, 2021，Views：1129
Original protocol used in research article：
Osmolarity-independent electrical cues guide rapid response to injury in zebrafish epidermis
eLife, Nov 23, 2020; DOI: 10.7554/eLife.62386
Real-Time PCR
Authors: Ming Xu and James L Kirkland
Date: Jun 3, 2021，Views：972
Original protocol used in research article：
Targeting senescent cells enhances adipogenesis and metabolic function in old age
eLife, Dec 19, 2015; DOI: 10.7554/eLife.12997
Reagents and antibodies
Author: Hongyu Deng
Date: Jun 3, 2021，Views：821
Original protocol used in research article：
PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasion
Science Advances, Nov 19, 2020; DOI: 10.1126/sciadv.abd0276
Histone modification ChIP-seq on Arabidopsis thaliana plantlets
Authors: Juan Sebastian Ramirez Prado, David Latrasse and Moussa Benhamed
Date: Jun 3, 2021，Views：1222
Original protocol used in research article：
A new role for histone demethylases in the maintenance of plant genome integrity
eLife, Oct 27, 2020; DOI: 10.7554/eLife.58533
Mice
Author: Bin Zhou
Date: Jun 3, 2021，Views：873
Original protocol used in research article：
Proliferation tracing reveals regional hepatocyte generation in liver homeostasis and repair
Science, Feb 26, 2021; DOI: 10.1126/science.abc4346
Culturing splenic, hepatic and alveolar macrophages
Author: Alexandre A Steiner
Date: Jun 2, 2021，Views：827
Original protocol used in research article：
A leukotriene-dependent spleen-liver axis drives TNF production in systemic inflammation
Science Signaling, Apr 20, 2021; DOI: 10.1126/scisignal.abb0969
Study design
Authors: Adam Yala and Regina Barzilay
Date: Jun 1, 2021，Views：921
Original protocol used in research article：
Toward robust mammography-based models for breast cancer risk
Science Translational Medicine, Jan 27, 2021; DOI: 10.1126/scitranslmed.aba4373
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