Protocols shared by authors via RaP service
Journal
Protein expression in PAO1 and cells lysis
Authors: Lital Alfonta and Eden Ozer
Date: May 14, 2023，Views：326
Original protocol used in research article：
An inside look at a biofilm: Pseudomonas aeruginosa flagella biotracking
Science Advances, Jun 11, 2021; DOI: 10.1126/sciadv.abg8581
BUT measurement and corneal damage scoring
Authors: Masatoshi Miyamoto, Takayuki Sassa and Akio Kihara
Date: May 13, 2023，Views：385
Original protocol used in research article：
Lipid polarity gradient formed by ω-hydroxy lipids in tear film prevents dry eye disease
eLife, Apr 7, 2020; DOI: 10.7554/eLife.53582
DR-GFP reporter assay
Authors: Calvin Shun Yu Lo and Nitika Taneja
Date: May 12, 2023，Views：491
Original protocol used in research article：
SMARCAD1-mediated active replication fork stability maintains genome integrity
Science Advances, May 5, 2021; DOI: 10.1126/sciadv.abe7804
PZQ treatment in in vitro cultured S. mansoni worms
Authors: Winka Le Clec’h and Timothy J. C. Anderson
Date: May 12, 2023，Views：348
Original protocol used in research article：
Genetic analysis of praziquantel response in schistosome parasites implicates a transient receptor potential channel
Science Translational Medicine, Dec 22, 2021; DOI: 10.1126/scitranslmed.abj9114
Real-time reverse transcription (RT)-PCR
Authors: Shahanshah Khan and Hasan Zaki
Date: May 12, 2023，Views：311
Original protocol used in research article：
SARS-CoV-2 spike protein induces inflammation via TLR2-dependent activation of the NF-κB pathway
eLife, Dec 6, 2021; DOI: 10.7554/eLife.68563
Biochemical measurements of ATP and ADP
Author: Masak Takaine
Date: May 11, 2023，Views：419
Original protocol used in research article：
High and stable ATP levels prevent aberrant intracellular protein aggregation in yeast
eLife, Apr 19, 2022; DOI: 10.7554/eLife.67659
DGE analysis
Authors: Martin Jinye Zhang, Angela Oliveira Pisco and James Zou
Date: May 10, 2023，Views：380
Original protocol used in research article：
Mouse aging cell atlas analysis reveals global and cell type-specific aging signatures
eLife, Apr 13, 2021; DOI: 10.7554/eLife.62293
Interbacterial competition assay
Authors: Ethel Bayer-Santos and Daniel Enrique Sanchez-Limache
Date: May 10, 2023，Views：454
Original protocol used in research article：
Antibacterial T6SS effectors with a VRR-Nuc domain are structure-specific nucleases
eLife, Oct 13, 2022; DOI: 10.7554/eLife.82437
An optimized four-step genome assembly strategy
Authors: Shilin Tian and Huabin Zhao
Date: May 10, 2023，Views：437
Original protocol used in research article：
Comparative analyses of bat genomes identify distinct evolution of immunity in Old World fruit bats
Science Advances, May 5, 2023; DOI: 10.1126/sciadv.add0141
DEV analysis
Authors: Florian Grziwotz, Arndt Telschow and Chih-hao Hsieh
Date: May 10, 2023，Views：22
Original protocol used in research article：
Anticipating the occurrence and type of critical transitions
Science Advances, Jan 6, 2023; DOI: 10.1126/sciadv.abq4558
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.