Bio-101 - Improve Research Reproducibility

Sortase A conjugation of HIV-1 envelope trimers to ferritin nanoparticles

Author: Kevin O. Saunders
Date: Jun 13, 2023，Views：931

Original protocol used in research article：

Targeted selection of HIV-specific antibody mutations by engineering B cell maturation
Science, Dec 5, 2019; DOI: 10.1126/science.aay7199

crystal fish imaging

Authors: Francois Kroll and Jason Rihel
Date: Jun 13, 2023，Views：901

Original protocol used in research article：

A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
eLife, Jan 8, 2021; DOI: 10.7554/eLife.59683

2.3.5. The Visual Analog Scale for Irritable Bowel Syndrome Questionnaire

Authors: Clara Nilholm and Bodil Ohlsson
Date: Jun 11, 2023，Views：26

Original protocol used in research article：

Assessment of a 4-Week Starch- and Sucrose-Reduced Diet and Its Effects on Gastrointestinal Symptoms and Inflammatory Parameters among Patients with Irritable Bowel Syndrome
Nutrients, Jan 28, 2021; DOI: 10.3390/nu13020416

CUMS model

Authors: Shu Yan Yu, Ye Li, Cuiqin Fan and Changmin Wang
Date: Jun 11, 2023，Views：1014

Original protocol used in research article：

Stress-induced reduction of Na+/H+ exchanger isoform 1 promotes maladaptation of neuroplasticity and exacerbates depressive behaviors
Science Advances, Nov 11, 2022; DOI: 10.1126/sciadv.add7063

Generation of SC2-VLPs - Protocol for transfection and filtering in 96well format

Authors: Melanie Ott and Jennifer A. Doudna
Date: Jun 11, 2023，Views：1057

Original protocol used in research article：

Rapid assessment of SARS-CoV-2 evolved variants using virus-like particles
Science, Nov 4, 2021; DOI: 10.1126/science.abl6184

Luciferase-based cytotoxicity assay

Authors: Gernot Stuhler and Boris Nowotny
Date: Jun 8, 2023，Views：1740

Original protocol used in research article：

Combinatorial targeting of multiple myeloma by complementing T cell engaging antibody fragments
Communications Biology, Jan 8, 2021; DOI: 10.1038/s42003-020-01558-0

Tissue processing and staining

Authors: Arnau Sans-Dublanc and Carlos Sindreu
Date: Jun 6, 2023，Views：791

Original protocol used in research article：

Septal GABAergic inputs to CA1 govern contextual memory retrieval
Science Advances, Oct 29, 2020; DOI: 10.1126/sciadv.aba5003

Tissue dissocication of zebrafish embryo with chorion

Authors: Huan Liu and Robert A Cornell
Date: Jun 5, 2023，Views：61

Original protocol used in research article：

Analysis of zebrafish periderm enhancers facilitates identification of a regulatory variant near human KRT8/18
eLife, Feb 7, 2020; DOI: 10.7554/eLife.51325

Single-cell RNA sequencing

Author: Stephen D Liberles
Date: Jun 2, 2023，Views：811

Original protocol used in research article：

Enteroendocrine cell lineages that differentially control feeding and gut motility
eLife, Feb 22, 2023; DOI: 10.7554/eLife.78512

Construction of plasmids and mutant strains

Author: Yukihiro Hiramatsu
Date: May 30, 2023，Views：25

Original protocol used in research article：

Interference of flagellar rotation up-regulates the expression of small RNA contributing to Bordetella pertussis infection
Science Advances, Dec 21, 2022; DOI: 10.1126/sciadv.ade8971

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