Protocols shared by authors via RaP service
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BG4 RIP-seq using adult mouse forebrain tissues
Authors: Sefan Asamitsu, Yasushi Yabuki and Norifumi Shioda
Date: Sep 7, 2023，Views：229
Original protocol used in research article：
RNA G-quadruplex organizes stress granule assembly through DNAPTP6 in neurons
Science Advances, Feb 24, 2023; DOI: 10.1126/sciadv.ade2035
Volumetric immunostaining and analysis of sympathetic nerves in tumors
Authors: Aeson Chang and Erica K. Sloan
Date: Sep 5, 2023，Views：139
Original protocol used in research article：
Beta-blockade enhances anthracycline control of metastasis in triple-negative breast cancer
Science Translational Medicine, Apr 26, 2023; DOI: 10.1126/scitranslmed.adf1147
Quantitative real-time polymerase chain reaction (qRT-PCR)
Authors: Yong-Hyun Han and Gwendalyn J. Randolph
Date: Sep 4, 2023，Views：134
Original protocol used in research article：
Enterically derived high-density lipoprotein restrains liver injury through the portal vein
Science, Jul 23, 2021; DOI: 10.1126/science.abe6729
RNase A treatment
Authors: Niladri K Sinha, Roland Beckmann and Rachel Green
Date: Sep 1, 2023，Views：208
Original protocol used in research article：
EDF1 coordinates cellular responses to ribosome collisions
eLife, Aug 3, 2020; DOI: 10.7554/eLife.58828
Isolation of synaptosomes and postsynaptic densities
Authors: M. Neal Waxham and Ilya Levental
Date: Sep 1, 2023，Views：269
Original protocol used in research article：
Remodeling of the postsynaptic plasma membrane during neural development
Molecular Biology of the Cell, Nov 7, 2016; DOI: 10.1091/mbc.e16-06-0420
TF transposon genotyping PCRs for granulosa differentiation
Authors: Merrick D Pierson Smela and George M Church
Date: Sep 1, 2023，Views：79
Original protocol used in research article：
Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression
eLife, Feb 21, 2023; DOI: 10.7554/eLife.83291
Animal experiments and echocardiography assessment
Authors: Dingli Xu and Qingchun Zeng
Date: Sep 1, 2023，Views：186
Original protocol used in research article：
Activation of Piezo1 promotes osteogenic differentiation of aortic valve interstitial cell through YAP-dependent glutaminolysis
Science Advances, Jun 2, 2023; DOI: 10.1126/sciadv.adg0478
Interpretation of Fractional Inhibitory Concentration Index (FICI)
Authors: Wenli Feng and Jing Yang
Date: Sep 1, 2023，Views：1266
Original protocol used in research article：
Cotreatment with Aspirin and Azole Drugs Increases Sensitivity of Candida albicans in vitro
Infect Drug Resist, Jun 2, 2021; DOI: 10.2147/IDR.S314538
Quantitative polymerase chain reaction (qPCR)
Authors: Ke-Hsuan Wei, Shih-Lei (Ben) Lai and I-Ting Lin
Date: Aug 31, 2023，Views：207
Original protocol used in research article：
Comparative single-cell profiling reveals distinct cardiac resident macrophages essential for zebrafish heart regeneration
eLife, Jul 27, 2023; DOI: 10.7554/eLife.84679
Cytokine release assay
Authors: Vanaja Konduri and William K. Decker
Date: Aug 31, 2023，Views：210
Original protocol used in research article：
A subset of cytotoxic effector memory T cells enhances CAR T cell efficacy in a model of pancreatic ductal adenocarcinoma
Science Translational Medicine, May 5, 2021; DOI: 10.1126/scitranslmed.abc3196
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