Protocols shared by authors via RaP service
Journal
Endogenous tagging of IRE1 in U-2 OS cells
Authors: Avi Ashkenazi and Vladislav Belyy
Date: Aug 2, 2023，Views：759
Original protocol used in research article：
Endoplasmic reticulum stress activates human IRE1α through reversible assembly of inactive dimers into small oligomers
eLife, ; DOI: 10.7554/eLife.74342
MD simulations
Authors: Talia Fargason and Jun Zhang
Date: Aug 1, 2023，Views：809
Original protocol used in research article：
Peptides that Mimic RS repeats modulate phase separation of SRSF1, revealing a reliance on combined stacking and electrostatic interactions
eLife, Mar 2, 2023; DOI: 10.7554/eLife.84412
Simultaneous profiling of chromosome conformation and gene expression in single cells
Authors: Yujie Chen, Heming Xu, Zhiyuan Liu and Dong Xing
Date: Aug 1, 2023，Views：843
Original protocol used in research article：
Linking genome structures to functions by simultaneous single-cell Hi-C and RNA-seq
SCIENCE, Jun 8, 2023; DOI: 10.1126/science.adg3797
Corneal tight junction integrity
Author: Qian Yu
Date: Jul 31, 2023，Views：69
Original protocol used in research article：
Canonical NF-κB signaling maintains corneal epithelial integrity and prevents corneal aging via retinoic acid
eLife, Jun 4, 2021; DOI: 10.7554/eLife.67315
Quantification and identification of cuticular hydrocarbons
Author: Trudy F C Mackay
Date: Jul 31, 2023，Views：745
Original protocol used in research article：
Genetic architecture of natural variation in cuticular hydrocarbon composition in Drosophila melanogaster
eLife, Nov 14, 2015; DOI: 10.7554/eLife.09861
SG-PERT
Author: Greg J Towers
Date: Jul 31, 2023，Views：778
Original protocol used in research article：
HIV-1 Vpr antagonizes innate immune activation by targeting karyopherin-mediated NF-κB/IRF3 nuclear transport
eLife, Dec 10, 2020; DOI: 10.7554/eLife.60821
Study design
Authors: JingHong Wan and Sophie Lotersztajn
Date: Jul 21, 2023，Views：845
Original protocol used in research article：
LC3-associated phagocytosis protects against inflammation and liver fibrosis via immunoreceptor inhibitory signaling
Science Translational Medicine, Apr 14, 2020; DOI: 10.1126/scitranslmed.aaw8523
Materials availability
Authors: Brielle Ferguson and John R Huguenard
Date: Jul 18, 2023，Views：955
Original protocol used in research article：
Prefrontal PV interneurons facilitate attention and are linked to attentional dysfunction in a mouse model of absence epilepsy
eLife, Apr 4, 2023; DOI: 10.7554/eLife.78349
Proteomics and untargeted metabolomics from plasma
Author: Kazuo Ando
Date: Jul 17, 2023，Views：946
Original protocol used in research article：
Integrated trajectories of the maternal metabolome, proteome, and immunome predict labor onset
Science Translational Medicine, May 5, 2021; DOI: 10.1126/scitranslmed.abd9898
Quantitative real-time PCR analysis
Author:
Date: Jul 15, 2023，Views：133
Original protocol used in research article：
ULK1-mediated metabolic reprogramming regulates Vps34 lipid kinase activity by its lactylation
Science Advances, Jun 2, 2023; DOI: 10.1126/sciadv.adg4993
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