Bio-101 - Improve Research Reproducibility

2.8. Observations of seed germination and seedling growth parameters

Author: Saiqa Andleeb
Date: Jul 15, 2023，Views：51

Original protocol used in research article：

Efficiency of cow dung based vermi-compost on seed germination and plant growth parameters of Tagetes erectus (Marigold)
Heliyon, Jan 6, 2021; DOI: 10.1016/j.heliyon.2020.e05895

Nystatin and subunit vaccine injection in mice

Author: Beth Ann Jiron Tamburini
Date: Jul 14, 2023，Views：990

Original protocol used in research article：

Molecular tracking devices quantify antigen distribution and archiving in the murine lymph node
eLife, Apr 12, 2021; DOI: 10.7554/eLife.62781

High-resolution 3D tissue imaging

Authors: Hua Zhang, Lu Mu, Xueqiang Xu, Xuebing Yang and Yan Zhang
Date: Jul 14, 2023，Views：1016

Original protocol used in research article：

Imaging and tracing the pattern of adult ovarian angiogenesis implies a strategy against female reproductive aging
Science Advances, Jan 12, 2022; DOI: 10.1126/sciadv.abi8683

Generation of SH-SY5Y HTT knockout cell lines by CRISPR-Cas9

Author: Jianning Wei
Date: Jul 13, 2023，Views：985

Original protocol used in research article：

RNA-seq analysis reveals significant transcriptome changes in huntingtin-null human neuroblastoma cells
BMC Medical Genomics, Jul 2, 2021; DOI: 10.1186/s12920-021-01022-w

HepaRG culture

Author: Francesco Bellanti
Date: Jul 13, 2023，Views：879

Original protocol used in research article：

Inhibition of nuclear factor (erythroid-derived 2)-like 2 promotes hepatic progenitor cell activation and differentiation
NPJ Regenerative Medicine, May 26, 2021; DOI: 10.1038/s41536-021-00137-z

MAG Bead DNA Extraction Protocol

Authors: Tamara D. Simon and Christopher E. Pope
Date: Jul 11, 2023，Views：744

Original protocol used in research article：

Characterization of cerebrospinal fluid (CSF) microbiota from patients with CSF shunt infection and reinfection using high throughput sequencing of 16S ribosomal RNAgenes
PLoS ONE, Jan 6, 2021; DOI: 10.1371/journal.pone.0244643

CyTOF analysis

Author: Chu-Xia Deng
Date: Jul 11, 2023，Views：741

Original protocol used in research article：

Cullin-5 deficiency orchestrates the tumor microenvironment to promote mammary tumor development through CREB1-CCL2 signaling
Science Advances, Jan 20, 2023; DOI: 10.1126/sciadv.abq1395

Analysis of differential expression in tumor and matched normal tissues

Author: Ying Yuan
Date: Jul 1, 2023，Views：751

Original protocol used in research article：

Pan-cancer analyses demonstrate that ANKRD6 is associated with a poor prognosis and correlates with M2 macrophage infiltration in colon cancer
Chinese Journal of Cancer Research, Feb 28, 2021; DOI: 10.21147/j.issn.1000-9604.2021.01.10

Immunofluorescence, immunoprecipitation, and ELISA using the Scicons anti-dsRNA J2 antibody

Authors: Elizabeth Ochoa and Bess Frost
Date: Jun 22, 2023，Views：972

Original protocol used in research article：

Pathogenic tau–induced transposable element–derived dsRNA drives neuroinflammation
Science Advances, Jan 6, 2023; DOI: 10.1126/sciadv.abq5423

APEX2 biotinylation in brain slices

Authors: David Sulzer and Peter A Sims
Date: Jun 21, 2023，Views：984

Original protocol used in research article：

Subcellular proteomics of dopamine neurons in the mouse brain
eLife, Jan 31, 2022; DOI: 10.7554/eLife.70921

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