Protocols shared by authors via RaP service
Journal
Generation of nanobody dimers by sortase-mediated functionalization and click chemistry
Author: Leo Hanke
Date: May 22, 2023，Views：526
Original protocol used in research article：
Multivariate mining of an alpaca immune repertoire identifies potent cross-neutralizing SARS-CoV-2 nanobodies
Science Advances, Mar 25, 2022; DOI: 10.1126/sciadv.abm0220
Differentiation of rTTA/Ngn2-positive PSCs to iNeurons
Authors: Maria Schörnig, Valentina Rava and Elena Taverna
Date: May 22, 2023，Views：588
Original protocol used in research article：
Comparison of induced neurons reveals slower structural and functional maturation in humans than in apes
eLife, Jan 20, 2021; DOI: 10.7554/eLife.59323
Sequences (Figs 1-3)
Authors: Morgane Rolland and Eric Lewitus
Date: May 17, 2023，Views：11
Original protocol used in research article：
Design of a pan-betacoronavirus vaccine candidate through a phylogenetically informed approach
Science Advances, Jan 18, 2023; DOI: 10.1126/sciadv.abq4149
EGFR Internalization and EGFR Recycling Assays
Authors: Chia-Hsing Shen and Li-Chung Hsu
Date: May 17, 2023，Views：667
Original protocol used in research article：
ZNRF1 Mediates Epidermal Growth Factor Receptor Ubiquitination to Control Receptor Lysosomal Trafficking and Degradation
Frontiers in Cell and Developmental Biology, Apr 29, 2021; DOI: 10.3389/fcell.2021.642625
Denaturing in vivo ubiquitination assay
Authors: Tyler B Faust, Amit Weiss, Bhargavi Jayaraman, Billy W Newton, Nevan J Krogan, Iván D'Orso and Alan D Frankel
Date: May 16, 2023，Views：660
Original protocol used in research article：
The HIV-1 Tat protein recruits a ubiquitin ligase to reorganize the 7SK snRNP for transcriptional activation
eLife, May 30, 2018; DOI: 10.7554/eLife.31879
RNAseq
Authors: Laura Amado-Rodríguez and Guillermo M Albaiceta
Date: May 16, 2023，Views：14
Original protocol used in research article：
Effects of IFIH1 rs1990760 variants on systemic inflammation and outcome in critically ill COVID-19 patients in an observational translational study
eLife, Jan 21, 2022; DOI: 10.7554/eLife.73012
The Transgression-Related Interpersonal Motivations Inventory (TRIM-18; McCullough et al., 1998, 2006)
Author: Pilar Martinez-Diaz
Date: May 16, 2023，Views：16
Original protocol used in research article：
Victim's Perspective of Forgiveness Seeking Behaviors After Transgressions
Frontiers in Psychology, Apr 21, 2021; DOI: 10.3389/fpsyg.2021.656689
Intracellular cytokine staining
Authors: Kizzmekia S. Corbett, Barney S. Graham and Robert A. Seder
Date: May 15, 2023，Views：461
Original protocol used in research article：
Immune correlates of protection by mRNA-1273 vaccine against SARS-CoV-2 in nonhuman primates
Science, Jul 29, 2021; DOI: 10.1126/science.abj0299
Protein expression and purification
Authors: Donghyuk Shin and Ivan Dikic
Date: May 14, 2023，Views：545
Original protocol used in research article：
Bacterial OTU deubiquitinases regulate substrate ubiquitination upon Legionella infection
eLife, Nov 13, 2020; DOI: 10.7554/eLife.58277
Mendelian randomization and sensitivity analyses
Author: Guillaume Paré
Date: May 14, 2023，Views：29
Original protocol used in research article：
GWAS and ExWAS of blood mitochondrial DNA copy number identifies 71 loci and highlights a potential causal role in dementia
eLife, Jan 13, 2022; DOI: 10.7554/eLife.70382
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