Protocols shared by authors via RaP service
Journal
2.8. Mitochondrial membrane potential (ΔΨm) and mtROS
Authors: Sehwan Jang and Sabzali Javadov
Date: Aug 31, 2023，Views：211
Original protocol used in research article：
Elucidating the contribution of mitochondrial glutathione to ferroptosis in cardiomyocytes
Redox Biology, Jun 1, 2021; DOI: 10.1016/j.redox.2021.102021
Tryptophan-quenching experiments
Author: Krishnananda Chattopadhyay
Date: Aug 29, 2023，Views：273
Original protocol used in research article：
The metal cofactor zinc and interacting membranes modulate SOD1 conformation-aggregation landscape in an in vitro ALS model
eLife, Apr 7, 2021; DOI: 10.7554/eLife.61453
En-bloc sample preparation for 3D electron microscopy
Authors: Ali Karimi and Moritz Helmstaedter
Date: Aug 29, 2023，Views：72
Original protocol used in research article：
Cell-type specific innervation of cortical pyramidal cells at their apical dendrites
eLife, Feb 28, 2020; DOI: 10.7554/eLife.46876
Efferocytosis assay
Authors: Zihao Yao and Haiyan Zhang
Date: Aug 27, 2023，Views：470
Original protocol used in research article：
Down-regulated GAS6 impairs synovial macrophage efferocytosis and promotes obesity-associated osteoarthritis
eLife, May 5, 2023; DOI: 10.7554/eLife.83069
MNase assay with EZ Nucleosomal DNA prep Kit
Authors: Yu-Xuan Lu and Linda Partridge
Date: Aug 20, 2023，Views：438
Original protocol used in research article：
A TORC1-histone axis regulates chromatin organisation and non-canonical induction of autophagy to ameliorate ageing
eLife, May 14, 2021; DOI: 10.7554/eLife.62233
Generation of pembrolizumab scFv
Authors: Zachary Davis and Jeffrey S. Miller
Date: Aug 16, 2023，Views：66
Original protocol used in research article：
Low-density PD-1 expression on resting human natural killer cells is functional and upregulated after transplantation
Blood Advances, Feb 18, 2021; DOI: 10.1182/bloodadvances.2019001110
HaloTag labeling
Author: Benjamin S Glick
Date: Aug 11, 2023，Views：651
Original protocol used in research article：
A microscopy-based kinetic analysis of yeast vacuolar protein sorting
eLife, Jun 25, 2020; DOI: 10.7554/eLife.56844
Immunohistochemistry and imaging of adrenal slices
Authors: Alik S. Widge and Polina Anikeeva
Date: Aug 7, 2023，Views：611
Original protocol used in research article：
Transgene-free remote magnetothermal regulation of adrenal hormones
Science Advances, Apr 9, 2020; DOI: 10.1126/sciadv.aaz3734
Yeast Two-Hybrid Assay
Author: Biao Liu
Date: Aug 2, 2023，Views：782
Original protocol used in research article：
Foreign Cry1Ab/c Delays Flowering in Insect-Resistant Transgenic Rice via Interaction With Hd3a Florigen
Frontiers in Plant Science, Feb 11, 2021; DOI: 10.3389/fpls.2021.608721
Endogenous tagging of IRE1 in U-2 OS cells
Authors: Avi Ashkenazi and Vladislav Belyy
Date: Aug 2, 2023，Views：759
Original protocol used in research article：
Endoplasmic reticulum stress activates human IRE1α through reversible assembly of inactive dimers into small oligomers
eLife, ; DOI: 10.7554/eLife.74342
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