Protocols shared by authors via RaP service
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MNase assay with EZ Nucleosomal DNA prep Kit
Authors: Yu-Xuan Lu and Linda Partridge
Date: Aug 20, 2023，Views：361
Original protocol used in research article：
A TORC1-histone axis regulates chromatin organisation and non-canonical induction of autophagy to ameliorate ageing
eLife, May 14, 2021; DOI: 10.7554/eLife.62233
Generation of pembrolizumab scFv
Authors: Zachary Davis and Jeffrey S. Miller
Date: Aug 16, 2023，Views：9
Original protocol used in research article：
Low-density PD-1 expression on resting human natural killer cells is functional and upregulated after transplantation
Blood Advances, Feb 18, 2021; DOI: 10.1182/bloodadvances.2019001110
HaloTag labeling
Author: Benjamin S Glick
Date: Aug 11, 2023，Views：468
Original protocol used in research article：
A microscopy-based kinetic analysis of yeast vacuolar protein sorting
eLife, Jun 25, 2020; DOI: 10.7554/eLife.56844
Immunohistochemistry and imaging of adrenal slices
Authors: Alik S. Widge and Polina Anikeeva
Date: Aug 7, 2023，Views：588
Original protocol used in research article：
Transgene-free remote magnetothermal regulation of adrenal hormones
Science Advances, Apr 9, 2020; DOI: 10.1126/sciadv.aaz3734
Yeast Two-Hybrid Assay
Author: Biao Liu
Date: Aug 2, 2023，Views：720
Original protocol used in research article：
Foreign Cry1Ab/c Delays Flowering in Insect-Resistant Transgenic Rice via Interaction With Hd3a Florigen
Frontiers in Plant Science, Feb 11, 2021; DOI: 10.3389/fpls.2021.608721
Endogenous tagging of IRE1 in U-2 OS cells
Authors: Avi Ashkenazi and Vladislav Belyy
Date: Aug 2, 2023，Views：695
Original protocol used in research article：
Endoplasmic reticulum stress activates human IRE1α through reversible assembly of inactive dimers into small oligomers
eLife, ; DOI: 10.7554/eLife.74342
MD simulations
Authors: Talia Fargason and Jun Zhang
Date: Aug 1, 2023，Views：754
Original protocol used in research article：
Peptides that Mimic RS repeats modulate phase separation of SRSF1, revealing a reliance on combined stacking and electrostatic interactions
eLife, Mar 2, 2023; DOI: 10.7554/eLife.84412
Simultaneous profiling of chromosome conformation and gene expression in single cells
Authors: Yujie Chen, Heming Xu, Zhiyuan Liu and Dong Xing
Date: Aug 1, 2023，Views：761
Original protocol used in research article：
Linking genome structures to functions by simultaneous single-cell Hi-C and RNA-seq
SCIENCE, Jun 8, 2023; DOI: 10.1126/science.adg3797
Corneal tight junction integrity
Author: Qian Yu
Date: Jul 31, 2023，Views：11
Original protocol used in research article：
Canonical NF-κB signaling maintains corneal epithelial integrity and prevents corneal aging via retinoic acid
eLife, Jun 4, 2021; DOI: 10.7554/eLife.67315
Quantification and identification of cuticular hydrocarbons
Author: Trudy F C Mackay
Date: Jul 31, 2023，Views：701
Original protocol used in research article：
Genetic architecture of natural variation in cuticular hydrocarbon composition in Drosophila melanogaster
eLife, Nov 14, 2015; DOI: 10.7554/eLife.09861
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