Protocols shared by authors via RaP service
Journal
scrambled RNPs
Authors: François Kroll and Jason Rihel
Date: Aug 19, 2022，Views：945
Original protocol used in research article：
A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
eLife, Jan 8, 2021; DOI: 10.7554/eLife.59683
Population structure and signatures of selection
Author: Luke Thomas
Date: Aug 18, 2022，Views：612
Original protocol used in research article：
Spatially varying selection between habitats drives physiological shifts and local adaptation in a broadcast spawning coral on a remote atoll in Western Australia
Science Advances, Apr 27, 2022; DOI: 10.1126/sciadv.abl9185
Does TRH increase LSNA or BAT SNA via actions in the DMH and/or RPa?
Authors: Zhigang Shi, Daniel L Marks and Virginia L Brooks
Date: Aug 18, 2022，Views：685
Original protocol used in research article：
Leptin increases sympathetic nerve activity via induction of its own receptor in the paraventricular nucleus
eLife, Jun 15, 2020; DOI: 10.7554/eLife.55357
FtsZ and GpsB purification
Authors: Prahathees J Eswara and Kumaran S Ramamurthi
Date: Aug 18, 2022，Views：713
Original protocol used in research article：
An essential Staphylococcus aureus cell division protein directly regulates FtsZ dynamics
eLife, Oct 2, 2018; DOI: 10.7554/eLife.38856
TraDIS method.
Authors: Karthik Pullela, Ian R. Henderson, Alessandra Polissi and Waldemar Vollmer
Date: Aug 17, 2022，Views：643
Original protocol used in research article：
DpaA Detaches Braun’s Lipoprotein from Peptidoglycan
mBio, May 4, 2021; DOI: 10.1128/mbio.00836-21
In vitro culture on tissue culture plastic, fibroblasts, or keratinocytes
Authors: Tiago R. Matos, Jessica E. Teague and Rachael A. Clark
Date: Aug 16, 2022，Views：1517
Original protocol used in research article：
Central memory T cells are the most effective precursors of resident memory T cells in human skin
Science Immunology, Apr 22, 2022; DOI: 10.1126/sciimmunol.abn1889
Diversity based on T cell composition
Authors: Liangtao Zheng, Shishang Qin, Xueda Hu and Zemin Zhang
Date: Aug 15, 2022，Views：694
Original protocol used in research article：
Pan-cancer single-cell landscape of tumor-infiltrating T cells
Science, Dec 17, 2021; DOI: 10.1126/science.abe6474
Indisulam efficacy studies in in vivo models
Authors: Shivendra Singh, Waise Quarni, Deepak Nijhawan, Andrew M. Davidoff and Jun Yang
Date: Aug 15, 2022，Views：643
Original protocol used in research article：
Targeting the spliceosome through RBM39 degradation results in exceptional responses in high-risk neuroblastoma models
Science Advances, Nov 17, 2021; DOI: 10.1126/sciadv.abj5405
In vitro phase separation assay
Authors: Yu Zhang, Lisha Shen and Hao Yu
Date: Aug 14, 2022，Views：953
Original protocol used in research article：
Phase separation of HRLP regulates flowering time in Arabidopsis
Science Advances, Jun 22, 2022; DOI: 10.1126/sciadv.abn5488
Plaque assays and immunostaining.
Authors: Chengjin Ye and Luis Martinez-Sobrido
Date: Aug 12, 2022，Views：782
Original protocol used in research article：
Generation and Characterization of Recombinant SARS-CoV-2 Expressing Reporter Genes
Journal of Virology, Mar 10, 2021; DOI: 10.1128/JVI.02209-20
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