Protocols shared by authors via RaP service
Journal
Ex vivo OT-1 cross-priming assay and secreted cytokine measurement
Authors: Rodney Cheng-En Hsieh and Michael A. Curran
Date: Jul 9, 2022，Views：591
Original protocol used in research article：
ATR-mediated CD47 and PD-L1 up-regulation restricts radiotherapy-induced immune priming and abscopal responses in colorectal cancer
Science Immunology, Jun 10, 2022; DOI: 10.1126/sciimmunol.abl9330
Bulk RNA-seq
Authors: Thanh Hoang, Jie Wang, David R. Hyde, Jiang Qian, Seth Blackshaw and Patrick Boyd
Date: Jul 8, 2022，Views：600
Original protocol used in research article：
Gene regulatory networks controlling vertebrate retinal regeneration
Science, Oct 1, 2020; DOI: 10.1126/science.abb8598
Histological examination
Authors: Francesco Zorzato and Alexis Ruiz
Date: Jul 8, 2022，Views：586
Original protocol used in research article：
Improvement of muscle strength in a mouse model for congenital myopathy treated with HDAC and DNA methyltransferase inhibitors
eLife, Mar 3, 2022; DOI: 10.7554/eLife.73718
Adoptive transfer
Authors: Federica Piattini and Manfred Kopf
Date: Jul 8, 2022，Views：564
Original protocol used in research article：
Monocyte-derived alveolar macrophages autonomously determine severe outcome of respiratory viral infection
Science Immunology, Jul 1, 2022; DOI: 10.1126/sciimmunol.abj5761
Study design
Authors: Tian-tuo Zhang, Hui Li, Bo Peng and Xuan-xian Peng
Date: Jul 7, 2022，Views：581
Original protocol used in research article：
Glutamine promotes antibiotic uptake to kill multidrug-resistant uropathogenic bacteria
Science Translational Medicine, Dec 22, 2021; DOI: 10.1126/scitranslmed.abj0716
HU recovery assay
Authors: Sophie C van der Horst and Haico van Attikum
Date: Jul 7, 2022，Views：542
Original protocol used in research article：
Chl1 helicase controls replication fork progression by regulating dNTP pools
Life Science Alliance, Jan 11, 2022; DOI: 10.26508/lsa.202101153
2.4 Bioinformatic analysis
Authors: Brendha Truccollo, Declan Bolton and Catherine M Burgess
Date: Jul 7, 2022，Views：550
Original protocol used in research article：
Genetic characterisation of a subset of Campylobacter jejuni isolates from clinical and poultry sources in Ireland
PLoS ONE, Mar 9, 2021; DOI: 10.1371/journal.pone.0246843
Allelic exchange constructs
Authors: Eva S Istvan, Akhil Vaidya and Daniel E Goldberg
Date: Jul 6, 2022，Views：560
Original protocol used in research article：
Plasmodium Niemann-Pick type C1-related protein is a druggable target required for parasite membrane homeostasis
eLife, Mar 19, 2019; DOI: 10.7554/eLife.40529
Image acquisition and analysis
Authors: Ana C. Laly and John T. Connelly
Date: Jul 6, 2022，Views：553
Original protocol used in research article：
The keratin network of intermediate filaments regulates keratinocyte rigidity sensing and nuclear mechanotransduction
Science Advances, Jan 27, 2021; DOI: 10.1126/sciadv.abd6187
Tumor processing for CAF isolation
Authors: Yuki Miyai and Atsushi Enomoto
Date: Jul 5, 2022，Views：680
Original protocol used in research article：
Meflin-positive cancer-associated fibroblasts enhance tumor response to immune checkpoint blockade
Life Science Alliance, Mar 1, 2022; DOI: 10.26508/lsa.202101230
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