Protocols shared by authors via RaP service
Journal
Luciferase assay
Authors: Jochen Winterer and Gerhard Schratt
Date: Aug 29, 2022，Views：1895
Original protocol used in research article：
MicroRNA-138 controls hippocampal interneuron function and short-term memory in mice
eLife, Mar 15, 2022; DOI: 10.7554/eLife.74056
Genotyping dHT mice and real-time qPCR
Authors: Francesco Zorzato and Alexis Ruiz
Date: Aug 29, 2022，Views：828
Original protocol used in research article：
Improvement of muscle strength in a mouse model for congenital myopathy treated with HDAC and DNA methyltransferase inhibitors
eLife, Mar 3, 2022; DOI: 10.7554/eLife.73718
2.3.1. The Cognitive Impulsivity Suite (CIS)
Authors: Katharina Voigt and Antonio Verdejo-Garcia
Date: Aug 28, 2022，Views：916
Original protocol used in research article：
The Hunger Games: Homeostatic State-Dependent Fluctuations in Disinhibition Measured with a Novel Gamified Test Battery
Nutrients, Jun 10, 2021; DOI: 10.3390/nu13062001
Flow cytometry
Authors: Shuang Wang, Wei Wei and Guanghui Ma
Date: Aug 26, 2022，Views：633
Original protocol used in research article：
Macrophage-tumor chimeric exosomes accumulate in lymph node and tumor to activate the immune response and the tumor microenvironment
Science Translational Medicine, Oct 13, 2021; DOI: 10.1126/scitranslmed.abb6981
In vivo and ex vivo imaging
Authors: Shuang Wang, Wei Wei and Guanghui Ma
Date: Aug 26, 2022，Views：619
Original protocol used in research article：
Macrophage-tumor chimeric exosomes accumulate in lymph node and tumor to activate the immune response and the tumor microenvironment
Science Translational Medicine, Oct 13, 2021; DOI: 10.1126/scitranslmed.abb6981
T cell receptor beta chain (TRB) sequencing of CD8 T cells from LNs
Authors: Shuang Wang, Wei Wei and Guanghui Ma
Date: Aug 26, 2022，Views：660
Original protocol used in research article：
Macrophage-tumor chimeric exosomes accumulate in lymph node and tumor to activate the immune response and the tumor microenvironment
Science Translational Medicine, Oct 13, 2021; DOI: 10.1126/scitranslmed.abb6981
Immunofluorescence (Tryptase and PGP9.5) and immunohistochemistry (H4R)
Authors: Giada De Palma, Chiko Shimbori and Premysl Bercik
Date: Aug 24, 2022，Views：743
Original protocol used in research article：
Histamine production by the gut microbiota induces visceral hyperalgesia through histamine 4 receptor signaling in mice
Science Translational Medicine, Jul 27, 2022; DOI: 10.1126/scitranslmed.abj1895
Production of anti–CTLA-4×SIRPα heterodimer
Authors: Anli Zhang and Yang-Xin Fu
Date: Aug 24, 2022，Views：727
Original protocol used in research article：
Dual targeting of CTLA-4 and CD47 on Treg cells promotes immunity against solid tumors
Science Translational Medicine, Aug 4, 2021; DOI: 10.1126/scitranslmed.abg8693
Preparation of nano-Ag@erythrosomes
Authors: Zhuang Liu, Zhen Gu and Chao Wang
Date: Aug 22, 2022，Views：669
Original protocol used in research article：
Red blood cell–derived nanoerythrosome for antigen delivery with enhanced cancer immunotherapy
Science Advances, Oct 23, 2019; DOI: 10.1126/sciadv.aaw6870
PCR analysis of the targeting events for HDR, NHEJ, and multiple copy integration
Authors: Boris V. Skryabin, Timofey S. Rozhdestvensky and Juergen Brosius
Date: Aug 19, 2022，Views：686
Original protocol used in research article：
Pervasive head-to-tail insertions of DNA templates mask desired CRISPR-Cas9–mediated genome editing events
Science Advances, Feb 11, 2020; DOI: 10.1126/sciadv.aax2941
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