Protocols shared by authors via RaP service
Journal
Antitumor efficacy of CAR-Ts in a mouse xenograft model
Authors: Qian Xiao and Xiaolei Su
Date: Sep 29, 2022，Views：540
Original protocol used in research article：
Size-dependent activation of CAR-T cells
Science Immunology, Aug 5, 2022; DOI: 10.1126/sciimmunol.abl3995
Digital number encoding network
Authors: Gabrielle J Gutierrez and Sophie Denève
Date: Sep 29, 2022，Views：550
Original protocol used in research article：
Population adaptation in efficient balanced networks
eLife, Sep 24, 2019; DOI: 10.7554/eLife.46926
spGFP11::2HA::Ort nucleotide sequence
Authors: Tzu-Yang Lin and Chi-Hon Lee
Date: Sep 28, 2022，Views：527
Original protocol used in research article：
Antagonistic regulation by insulin-like peptide and activin ensures the elaboration of appropriate dendritic field sizes of amacrine neurons
eLife, Mar 16, 2020; DOI: 10.7554/eLife.50568
BN-PAGE
Authors: Nak Hyun Kim, Zhen-Ming Pei, Li Wan and Jeffery L. Dangl
Date: Sep 27, 2022，Views：580
Original protocol used in research article：
Plant “helper” immune receptors are Ca2+-permeable nonselective cation channels
Science, Jul 23, 2021; DOI: 10.1126/science.abg7917
Designing and cloning of the gRNA
Authors: Kumarasamypet M Mohankumar and Nithin Sam R
Date: Sep 27, 2022，Views：616
Original protocol used in research article：
Identification of novel HPFH-like mutations by CRISPR base editing that elevate the expression of fetal hemoglobin
eLife, Feb 11, 2022; DOI: 10.7554/eLife.65421
Preparation of single-cell suspensions
Author: Claire E. O’Leary
Date: Sep 26, 2022，Views：540
Original protocol used in research article：
Bile acid–sensitive tuft cells regulate biliary neutrophil influx
Science Immunology, Mar 4, 2022; DOI: 10.1126/sciimmunol.abj1080
Lentivirus infection of organoids to test infection efficiency
Authors: Dawei Sun and Emma L Rawlins
Date: Sep 26, 2022，Views：726
Original protocol used in research article：
A functional genetic toolbox for human tissue-derived organoids
eLife, Oct 6, 2021; DOI: 10.7554/eLife.67886
LC-MS/MS sample preparation
Authors: Jonathan G Van Vranken and Steven P Gygi
Date: Sep 26, 2022，Views：638
Original protocol used in research article：
Assessing target engagement using proteome-wide solvent shift assays
eLife, Dec 8, 2021; DOI: 10.7554/eLife.70784
In vitro pull-down assay
Authors: Yue Xiong and Yan-ping Xu
Date: Sep 25, 2022，Views：568
Original protocol used in research article：
USP15 suppresses tumor immunity via deubiquitylation and inactivation of TET2
Science Advances, Sep 17, 2020; DOI: 10.1126/sciadv.abc9730
Competitive fitness assays
Authors: Hala Tamim El Jarkass and Aaron W Reinke
Date: Sep 23, 2022，Views：550
Original protocol used in research article：
An intestinally secreted host factor promotes microsporidia invasion of C. elegans
eLife, Jan 7, 2022; DOI: 10.7554/eLife.72458
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