Bio-101 - Improve Research Reproducibility

Glucose (2-NBDG) uptake assay

Authors: Arpankumar Choksi and Samit Chattopadhyay
Date: Sep 17, 2022，Views：791

Original protocol used in research article：

Tumor suppressor SMAR1 regulates PKM alternative splicing by HDAC6-mediated deacetylation of PTBP1
Cancer & Metabolism, Apr 16, 2021; DOI: 10.1186/s40170-021-00252-x

Sporulation Test

Authors: Victor Chinomso Ujor, Venkat Gopalan and Thaddeus Chukwuemeka Ezeji
Date: Sep 16, 2022，Views：581

Original protocol used in research article：

Ribozyme-Mediated Downregulation Uncovers DNA Integrity Scanning Protein A (DisA) as a Solventogenesis Determinant in Clostridium beijerinckii
Frontiers in Bioengineering and Biotechnology, Jun 8, 2021; DOI: 10.3389/fbioe.2021.669462

Plaque Reduction Neutralization Test (PRNT)

Authors: Sai Luo, Tom Kirchhausen, Bing Chen, Barton F. Haynes and Frederick W. Alt
Date: Sep 16, 2022，Views：548

Original protocol used in research article：

An Antibody from Single Human VH-rearranging Mouse Neutralizes All SARS-CoV-2 Variants Through BA.5 by Inhibiting Membrane Fusion
Science Immunology, Aug 11, 2022; DOI: 10.1126/sciimmunol.add5446

LX-2 cell culture

Author: Scott L. Friedman
Date: Sep 16, 2022，Views：835

Original protocol used in research article：

Aramchol downregulates stearoyl CoA-desaturase 1 in hepatic stellate cells to attenuate cellular fibrogenesis
JHEP Reports, Jan 28, 2021; DOI: 10.1016/j.jhepr.2021.100237

Spatially particle-based simulation of FAKY397 activation in integrin clusters

Authors: Bo Cheng and Min Lin
Date: Sep 16, 2022，Views：533

Original protocol used in research article：

Nanoscale integrin cluster dynamics controls cellular mechanosensing via FAKY397 phosphorylation
Science Advances, Mar 4, 2020; DOI: 10.1126/sciadv.aax1909

Immunoblot

Authors: Ziru Li and Ormond A MacDougald
Date: Sep 14, 2022，Views：554

Original protocol used in research article：

Lipolysis of bone marrow adipocytes is required to fuel bone and the marrow niche during energy deficits
eLife, ; DOI: 10.7554/eLife.78496

RNA extraction and quantitative real-time PCR (qPCR)

Authors: Ziru Li and Ormond A MacDougald
Date: Sep 14, 2022，Views：551

Original protocol used in research article：

Lipolysis of bone marrow adipocytes is required to fuel bone and the marrow niche during energy deficits
eLife, ; DOI: 10.7554/eLife.78496

Bead Supported Lipid Bilayers (BSLB)

Authors: James H. Felce, Pablo F. Céspedes and Michael L. Dustin
Date: Sep 14, 2022，Views：529

Original protocol used in research article：

Single-Molecule, Super-Resolution, and Functional Analysis of G Protein-Coupled Receptor Behavior Within the T Cell Immunological Synapse
Frontiers in Cell and Developmental Biology, Jan 18, 2021; DOI: 10.3389/fcell.2020.608484

Cardiomyocyte fatty acid oxidation assay

Authors: Lucas C Pantaleão, Isabella Inzani and Susan E Ozanne
Date: Sep 14, 2022，Views：550

Original protocol used in research article：

Maternal diet-induced obesity during pregnancy alters lipid supply to mouse E18.5 fetuses and changes the cardiac tissue lipidome in a sex-dependent manner
eLife, Jan 13, 2022; DOI: 10.7554/eLife.69078

Puromycin treatment in the presence of other translation inhibitors

Authors: Benjamin D Hobson and Peter A Sims
Date: Sep 14, 2022，Views：628

Original protocol used in research article：

Elongation inhibitors do not prevent the release of puromycylated nascent polypeptide chains from ribosomes
eLife, Aug 26, 2020; DOI: 10.7554/eLife.60048

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