Key resource table

Chenhui Wang; Allan C Spradling

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Key resource table

CW Chenhui Wang

AS Allan C Spradling

Last updated date: Nov 30, 2022 Views: 917 Forks: 0

An abbreviated version of this protocol was published in eLife in Mar, 2020

An abundant quiescent stem cell population in Drosophila Malpighian tubules protects principal cells from kidney stones

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Key Resources Table
Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
genetic reagent (D. melanogaster)	ry⁵⁰⁶	Bloomington Drosophila Stock Center (BDSC)	RRID:BDSC_4405
genetic reagent (D. melanogaster)	Oregon-R	BDSC	BDSC:25211; RRID:BDSC_25211
genetic reagent (D. melanogaster)	N^55e11	BDSC	RRID:BDSC_28813
genetic reagent (D. melanogaster)	NRE-GFP	BDSC	RRID:BDSC_30727
genetic reagent (D. melanogaster)	UAS-NICD	BDSC	RRID:BDSC_52008
genetic reagent (D. melanogaster)	10Xstat-GFP	BDSC	RRID:BDSC_26198
genetic reagent (D. melanogaster)	tsh-lacZ	BDSC	RRID:BDSC_11370
genetic reagent (D. melanogaster)	Alp4-lacZ	BDSC	RRID:BDSC_12285
genetic reagent (D. melanogaster)	Diap1-lacZ	BDSC	RRID:BDSC_12093
genetic reagent (D. melanogaster)	ex-lacZ	BDSC	RRID:BDSC_44248
genetic reagent (D. melanogaster)	UAS-myrRFP	BDSC	RRID:BDSC_32222
genetic reagent (D. melanogaster)	UAS-ct	BDSC	RRID:BDSC_36496
genetic reagent (D. melanogaster)	ct-RNAi	BDSC	RRID:BDSC_29625
genetic reagent (D. melanogaster)	UAS-rpr,hid	(Zhou et al., 1997)
genetic reagent (D. melanogaster)	N-RNAi	BDSC	RRID:BDSC_35640
genetic reagent (D. melanogaster)	UAS-Rac1.N17	BDSC	RRID:BDSC_6292
genetic reagent (D. melanogaster)	FRT^19A	BDSC	RRID:BDSC_1709
genetic reagent (D. melanogaster)	FRT^42D	BDSC	RRID:BDSC_1802
genetic reagent (D. melanogaster)	Act5C>stop>lacZ	BDSC	RRID:BDSC_6355
genetic reagent (D. melanogaster)	UAS-FLP	BDSC	RRID:BDSC_4539
genetic reagent (D. melanogaster)	Puc^E69-Gal4	BDSC	RRID:BDSC_6762
genetic reagent (D. melanogaster)	c507-Gal4	BDSC	RRID:BDSC_30840
genetic reagent (D. melanogaster)	Uro-Gal4	BDSC	RRID:BDSC_44416
genetic reagent (D. melanogaster)	tsh-Gal4	BDSC	RRID:BDSC_3040
genetic reagent (D. melanogaster)	esg-Gal4	(Micchelli and Perrimon, 2006)
genetic reagent (D. melanogaster)	cad-Gal4	BDSC	RRID:BDSC_3042
genetic reagent (D. melanogaster)	tub-Gal80^ts	BDSC	RRID:BDSC_7018
genetic reagent (D. melanogaster)	tub-Gal80^ts	BDSC	RRID:BDSC_7019
genetic reagent (D. melanogaster)	Df31-GFP	(Buszczak et al., 2007)
genetic reagent (D. melanogaster)	Pvr-GFP	Vienna Drosophila Resource Center (VDRC)	RRID:VDRC_318162
genetic reagent (D. melanogaster)	UAS-upd1	J. Urban (X. Chen lab, JHU)
genetic reagent (D. melanogaster)	UAS-rCD2.RFP,UAS-GFPi, FRT40A	BDSC	RRID:BDSC_56184
genetic reagent (D. melanogaster)	UAS-rCD8.GFP,UAS-rCD2i,FRT40A	BDSC	RRID:BDSC_56185
genetic reagent (D. melanogaster)	42D-MARCM	(Fox and Spradling, 2009)		UAS-cd8GFP hsFlp; FRT^42D tub-Gal80; tub-Gal4/TM6B, Tb
antibody	anti-Cut (mouse monoclonal)	Developemental Studies Hybridoma Bank (DSHB)	Cat#2B10; RRID: AB_528186	IF(1:20)
antibody	anti-Dl (mouse monoclonal)	DSHB	Cat#C594.9B; RRID: AB_528194	IF(1:10)
antibody	anti-NECD (mouse monoclonal)	DSHB	Cat#C458.2H; RRID: AB_528408	IF(1:10)
antibody	anti-b-Gal (mouse monoclonal)	Promega	Cat#Z3781; AB_430877	IF(1:1000)
antibody	anti-b-Gal (rabbit polyclonal)	Cappel	Cat#55976; AB_2313707	IF(1:2000)
antibody	anti-GFP (rabbit polyclonal)	ThermoFisher Scientific	Cat# A-11122; RRID: AB_221569	IF(1:2000)
antibody	anti-PH3 (mouse polyclonal)	Cell Signaling Technology	Cat# 9706, RRID:AB_331748	IF(1:500)
antibody	anti-RFP (rabit polyclonal)	Rockland	Cat# 600401379, RRID: AB_2209751	IF(1:2000)
antibody	anti-dpErK (mouse polyclonal)	Sigma-Aldrich	Cat# M9692, RRID:AB_260729	IF(1:500)
antibody	Alexa 488 goat anti-Mouse	ThermoFisher Scientific	Cat#: A11001; RRID: AB_2534069	IF(1:300)
antibody	Alexa 568 goat anti-Mouse	ThermoFisher Scientific	Cat#: A11004; RRID: AB_2534072	IF(1:300)
antibody	Alexa 488 goat anti-Rabbit	ThermoFisher Scientific	Cat#: A11034; RRID: AB_2576271	IF(1:300)
antibody	Alexa 568 goat anti-Rabbit	ThermoFisher Scientific	Cat#: A11041; RRID: AB_2534098	IF(1:300)
commercial assay or kit	Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 488 dye	ThermoFisher	Cat#C10337
commercial assay or kit	TruSeq RNA Library Prep Kit v2	illumina	RS-122-2001
chemical compound, drug	Allopurinol	Sigma Aldrich	Cat#A8003
software, algorithm	IMARIS v9.2.1	Bitplane	RRID:SCR_007370
software, algorithm	Fiji	NIH	RRID:SCR_002285
software, algorithm	DESeq2	(Love et al., 2014)	RRID:SCR_015687
software, algorithm	HISAT2 v2.1.0	(Pertea et al., 2016)	RRID:SCR_015530
software, algorithm	Seurat	(Stuart et al., 2019)	RRID:SCR_016341
software, algorithm	Cell Ranger	10X genomics	RRID:SCR_017344

How to cite：

Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:

Wang, C and Spradling, A(2022). Key resource table. Bio-protocol Preprint. bio-protocol.org/prep2067.
Wang, C. and Spradling, A. C.(2020). An abundant quiescent stem cell population in Drosophila Malpighian tubules protects principal cells from kidney stones. eLife. DOI: 10.7554/eLife.54096

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