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Image acquisition and staining

RK Roza Kucharczyk
HB Hubert Dominique Becker

Last updated date: Nov 29, 2022 Views: 861 Forks: 0

original An abbreviated version of this protocol was published in eLife in Jul, 2020
Assigning mitochondrial localization of dual localized proteins using a yeast Bi-Genomic Mitochondrial-Split-GFP
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Dears,

here under this reference you will find the protocol for visualising the mitonetwork using Big Split GFP system:

"
Visualizing Mitochondrial Importability of a Protein Using the Yeast Bi-Genomic Mitochondrial-Split-GFP Strain and an Ordinary Fluorescence Microscope.

Hemmerle M, Senger B, Rago JD, Kucharczyk R, Becker HD.Methods Mol Biol. 2022;2497:255-267. doi: 10.1007/978-1-0716-2309-1_16.PMID: 35771447 Free article."

Regards, Roza Kucharczyk

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Kucharczyk, R and Becker, H(2022). Image acquisition and staining. Bio-protocol Preprint. bio-protocol.org/prep2062.
  2. Bader, G., Enkler, L., Araiso, Y., Hemmerle, M., Binko, K., Baranowska, E., De Craene, J., Ruer-Laventie, J., Pieters, J., Tribouillard-Tanvier, D., Senger, B., di Rago, J., Friant, S., Kucharczyk, R. and Becker, H. D.(2020). Assigning mitochondrial localization of dual localized proteins using a yeast Bi-Genomic Mitochondrial-Split-GFP. eLife. DOI: 10.7554/eLife.56649

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