Improve Research Reproducibility A Bio-protocol resource
bpdetail_icon_lock
Preprint

Image acquisition and staining

RK Roza Kucharczyk
HB Hubert Dominique Becker

Last updated date: Nov 29, 2022 Views: 866 Forks: 0

original An abbreviated version of this protocol was published in eLife in Jul, 2020
Assigning mitochondrial localization of dual localized proteins using a yeast Bi-Genomic Mitochondrial-Split-GFP
download pdf Download PDF
ask Ask a question
cite How to cite
nfavorite Favorite

Dears,

here under this reference you will find the protocol for visualising the mitonetwork using Big Split GFP system:

"
Visualizing Mitochondrial Importability of a Protein Using the Yeast Bi-Genomic Mitochondrial-Split-GFP Strain and an Ordinary Fluorescence Microscope.

Hemmerle M, Senger B, Rago JD, Kucharczyk R, Becker HD.Methods Mol Biol. 2022;2497:255-267. doi: 10.1007/978-1-0716-2309-1_16.PMID: 35771447 Free article."

Regards, Roza Kucharczyk

Copyright: Content may be subjected to copyright.
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Kucharczyk, R and Becker, H(2022). Image acquisition and staining. Bio-protocol Preprint. bio-protocol.org/prep2062.
  2. Bader, G., Enkler, L., Araiso, Y., Hemmerle, M., Binko, K., Baranowska, E., De Craene, J., Ruer-Laventie, J., Pieters, J., Tribouillard-Tanvier, D., Senger, B., di Rago, J., Friant, S., Kucharczyk, R. and Becker, H. D.(2020). Assigning mitochondrial localization of dual localized proteins using a yeast Bi-Genomic Mitochondrial-Split-GFP. eLife. DOI: 10.7554/eLife.56649

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A
This protocol preprint was submitted via the Bio-protocol Exchange "Submit a Preprint" track.