Bio-101 - Improve Research Reproducibility

Immunoblot

Authors: Ziru Li and Ormond A MacDougald
Date: Sep 14, 2022，Views：664

Original protocol used in research article：

Lipolysis of bone marrow adipocytes is required to fuel bone and the marrow niche during energy deficits
eLife, ; DOI: 10.7554/eLife.78496

RNA extraction and quantitative real-time PCR (qPCR)

Authors: Ziru Li and Ormond A MacDougald
Date: Sep 14, 2022，Views：693

Original protocol used in research article：

Lipolysis of bone marrow adipocytes is required to fuel bone and the marrow niche during energy deficits
eLife, ; DOI: 10.7554/eLife.78496

Bead Supported Lipid Bilayers (BSLB)

Authors: James H. Felce, Pablo F. Céspedes and Michael L. Dustin
Date: Sep 14, 2022，Views：557

Original protocol used in research article：

Single-Molecule, Super-Resolution, and Functional Analysis of G Protein-Coupled Receptor Behavior Within the T Cell Immunological Synapse
Frontiers in Cell and Developmental Biology, Jan 18, 2021; DOI: 10.3389/fcell.2020.608484

Cardiomyocyte fatty acid oxidation assay

Authors: Lucas C Pantaleão, Isabella Inzani and Susan E Ozanne
Date: Sep 14, 2022，Views：604

Original protocol used in research article：

Maternal diet-induced obesity during pregnancy alters lipid supply to mouse E18.5 fetuses and changes the cardiac tissue lipidome in a sex-dependent manner
eLife, Jan 13, 2022; DOI: 10.7554/eLife.69078

Puromycin treatment in the presence of other translation inhibitors

Authors: Benjamin D Hobson and Peter A Sims
Date: Sep 14, 2022，Views：744

Original protocol used in research article：

Elongation inhibitors do not prevent the release of puromycylated nascent polypeptide chains from ribosomes
eLife, Aug 26, 2020; DOI: 10.7554/eLife.60048

BIOMOL ATP hydrolysis assay

Authors: Michael P Latham and Marella D Canny
Date: Sep 14, 2022，Views：667

Original protocol used in research article：

LRET-derived HADDOCK structural models describe the conformational heterogeneity required for DNA cleavage by the Mre11-Rad50 DNA damage repair complex
eLife, Jan 27, 2022; DOI: 10.7554/eLife.69579

Hema-3 Staining and Nuclear Morphology Analysis

Authors: Stephanie L. Silva-Del Toro and Lee-Ann H. Allen
Date: Sep 14, 2022，Views：977

Original protocol used in research article：

Microtubules and Dynein Regulate Human Neutrophil Nuclear Volume and Hypersegmentation During H. pylori Infection
Frontiers in Immunology, Mar 22, 2021; DOI: 10.3389/fimmu.2021.653100

Puromycin Immunofluorescence

Authors: Benjamin D Hobson and Peter A Sims
Date: Sep 14, 2022，Views：661

Original protocol used in research article：

Elongation inhibitors do not prevent the release of puromycylated nascent polypeptide chains from ribosomes
eLife, Aug 26, 2020; DOI: 10.7554/eLife.60048

Foetal cardiomyocyte size assessment

Authors: Lucas C Pantaleão, Isabella Inzani and Susan E Ozanne
Date: Sep 14, 2022，Views：592

Original protocol used in research article：

Limiting dilution transplantation

Authors: Syn Kok Yeo and Jun-Lin Guan
Date: Sep 14, 2022，Views：564

Original protocol used in research article：

Single-cell RNA-sequencing reveals distinct patterns of cell state heterogeneity in mouse models of breast cancer
eLife, Aug 25, 2020; DOI: 10.7554/eLife.58810

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