Protocols shared by authors via RaP service
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In vitro pull-down assay
Authors: Yue Xiong and Yan-ping Xu
Date: Sep 25, 2022，Views：578
Original protocol used in research article：
USP15 suppresses tumor immunity via deubiquitylation and inactivation of TET2
Science Advances, Sep 17, 2020; DOI: 10.1126/sciadv.abc9730
Competitive fitness assays
Authors: Hala Tamim El Jarkass and Aaron W Reinke
Date: Sep 23, 2022，Views：572
Original protocol used in research article：
An intestinally secreted host factor promotes microsporidia invasion of C. elegans
eLife, Jan 7, 2022; DOI: 10.7554/eLife.72458
ASC Speck Quantification and High Magnification Imaging
Authors: Hayley I. Muendlein and Alexander Poltorak
Date: Sep 22, 2022，Views：551
Original protocol used in research article：
cFLIPL protects macrophages from LPS-induced pyroptosis via inhibition of complex II formation
Science, Mar 19, 2020; DOI: 10.1126/science.aay3878
Fabrication of ORMs and catalase conjugation
Authors: Ya Guan, Hong Niu and Jianjun Guan
Date: Sep 22, 2022，Views：559
Original protocol used in research article：
Sustained oxygenation accelerates diabetic wound healing by promoting epithelialization and angiogenesis and decreasing inflammation
Science Advances, Aug 27, 2021; DOI: 10.1126/sciadv.abj0153
Synthesis and characterization of ROS-scavenging hydrogel
Authors: Ya Guan, Hong Niu and Jianjun Guan
Date: Sep 22, 2022，Views：581
Original protocol used in research article：
Sustained oxygenation accelerates diabetic wound healing by promoting epithelialization and angiogenesis and decreasing inflammation
Science Advances, Aug 27, 2021; DOI: 10.1126/sciadv.abj0153
Flow cytometry–based ICAM1-binding assay
Authors: Kristoffer H. Johansen, Klaus Okkenhaug and Pamela L. Schwartzberg
Date: Sep 21, 2022，Views：703
Original protocol used in research article：
A CRISPR screen targeting PI3K effectors identifies RASA3 as a negative regulator of LFA-1–mediated adhesion in T cells
Science Signaling, Jul 19, 2022; DOI: 10.1126/scisignal.abl9169
Supporting materials for "Standardized and reproducible measurement of decision-making in mice"
Author: International Brain Laboratory
Date: Sep 21, 2022，Views：644
Original protocol used in research article：
Standardized and reproducible measurement of decision-making in mice
eLife, May 20, 2021; DOI: 10.7554/eLife.63711
Cell adhesion assay
Authors: Bochuan Li, Ying Yang, Hongfeng Jiang and Degang Liang
Date: Sep 20, 2022，Views：774
Original protocol used in research article：
RNA N6-methyladenosine modulates endothelial atherogenic responses to disturbed flow in mice
eLife, Jan 10, 2022; DOI: 10.7554/eLife.69906
Immunofluorescence
Authors: Kristine Schauer, Mathieu Coppey and Kotryna Vaidziulyte
Date: Sep 20, 2022，Views：571
Original protocol used in research article：
Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking
eLife, Mar 18, 2022; DOI: 10.7554/eLife.69229
Primary hair cell culture
Authors: Louise Menendez, Justin Ichida and Neil Segil
Date: Sep 19, 2022，Views：585
Original protocol used in research article：
Generation of inner ear hair cells by direct lineage conversion of primary somatic cells
eLife, Jun 30, 2020; DOI: 10.7554/eLife.55249
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