Protocols shared by authors via RaP service
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Total RNA extraction and quantitative RT-PCR
Authors: Yan Tang and Jeffrey Pessin
Date: Sep 13, 2022，Views：569
Original protocol used in research article：
TIGAR deficiency enhances skeletal muscle thermogenesis by increasing neuromuscular junction cholinergic signaling
eLife, Mar 7, 2022; DOI: 10.7554/eLife.73360
Phylogenetics
Authors: Adrián A. Davín, Philip Hugenholtz, Gergely J. Szöllősi and Tom A. Williams
Date: Sep 13, 2022，Views：558
Original protocol used in research article：
A rooted phylogeny resolves early bacterial evolution
Science, May 7, 2021; DOI: 10.1126/science.abe0511
Immunofluorescence staining
Authors: Diptiman Chanda and Victor J Thannickal
Date: Sep 9, 2022，Views：570
Original protocol used in research article：
Mesenchymal stromal cell aging impairs the self-organizing capacity of lung alveolar epithelial stem cells
eLife, Sep 16, 2021; DOI: 10.7554/eLife.68049
Flow cytometry and cell sorting
Author: David A. Hildeman
Date: Sep 9, 2022，Views：530
Original protocol used in research article：
IL-10–producing Tfh cells accumulate with age and link inflammation with age-related immune suppression
Science Advances, Jul 29, 2020; DOI: 10.1126/sciadv.abb0806
In vivo single-cell mosaic labeling to visualize retinal neurons
Authors: Mai Ahmed and Ichiro Masai
Date: Sep 9, 2022，Views：690
Original protocol used in research article：
Strip1 regulates retinal ganglion cell survival by suppressing Jun-mediated apoptosis to promote retinal neural circuit formation
eLife, Mar 22, 2022; DOI: 10.7554/eLife.74650
Adhesion formation assay
Authors: Lindsay B Case and Michael K Rosen
Date: Sep 9, 2022，Views：542
Original protocol used in research article：
Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formation
eLife, Jan 20, 2022; DOI: 10.7554/eLife.72588
DNA staining, flow cytometry, and confocal microscopy
Author: David Sacks
Date: Sep 9, 2022，Views：528
Original protocol used in research article：
Stress conditions promote Leishmania hybridization in vitro marked by expression of the ancestral gamete fusogen HAP2 as revealed by single-cell RNA-seq
eLife, Jan 7, 2022; DOI: 10.7554/eLife.73488
Fabrication of the DOAMS
Authors: Wei Chen and Giovanni Traverso
Date: Sep 8, 2022，Views：532
Original protocol used in research article：
Dynamic omnidirectional adhesive microneedle system for oral macromolecular drug delivery
Science Advances, Jan 5, 2022; DOI: 10.1126/sciadv.abk1792
Unsupervised clustering of 4,000-gene human MERFISH data
Authors: Rongxin Fang and Xiaowei Zhuang
Date: Sep 8, 2022，Views：547
Original protocol used in research article：
Conservation and divergence of cortical cell organization in human and mouse revealed by MERFISH
Science, Jun 30, 2022; DOI: 10.1126/science.abm1741
Measurement of vaccine-induced IgA antibody mucosal samples
Authors: Stephanie N. Langel and Sean N. Tucker
Date: Sep 8, 2022，Views：547
Original protocol used in research article：
Adenovirus type 5 SARS-CoV-2 vaccines delivered orally or intranasally reduced disease severity and transmission in a hamster model
Science Translational Medicine, May 5, 2022; DOI: 10.1126/scitranslmed.abn6868
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