Protocols shared by authors via RaP service
Journal
Model building and refinement
Authors: Qiang Su and Yigong Shi
Date: Dec 25, 2022，Views：793
Original protocol used in research article：
Cryo-EM structure of the human IgM B cell receptor
Science, Aug 18, 2022; DOI: 10.1126/science.abo3923
S-specific immunoglobulin ELISA
Authors: Karen Colwill, Reuben Samson, Queenie Hu, Anne-Claude Gingras, Natalia Martin Orozco, Jun Liu, Eric G. Marcusson and Mario A. Ostrowski
Date: Dec 23, 2022，Views：828
Original protocol used in research article：
Preclinical evaluation of a SARS-CoV-2 mRNA vaccine PTX-COVID19-B
Science Advances, Jan 19, 2022; DOI: 10.1126/sciadv.abj9815
par-seqFISH
Authors: Daniel Dar, Long Cai and Dianne K. Newman
Date: Dec 20, 2022，Views：852
Original protocol used in research article：
Spatial transcriptomics of planktonic and sessile bacterial populations at single-cell resolution
Science, Aug 12, 2021; DOI: 10.1126/science.abi4882
Real-time quantitative PCR (qRT-PCR)
Authors: Gail Mandel and Tamilla Nechiporuk
Date: Dec 18, 2022，Views：1110
Original protocol used in research article：
The REST remodeling complex protects genomic integrity during embryonic neurogenesis
eLife, Jan 8, 2016; DOI: 10.7554/eLife.09584
Wire hang test
Authors: Michael A Petr and Rafael de Cabo
Date: Dec 18, 2022，Views：1825
Original protocol used in research article：
A cross-sectional study of functional and metabolic changes during aging through the lifespan in male mice
eLife, Apr 20, 2021; DOI: 10.7554/eLife.62952
HMT assay
Authors: Danny Reinberg|| and Chul-Hwan Lee
Date: Dec 16, 2022，Views：858
Original protocol used in research article：
Multiple modes of PRC2 inhibition elicit global chromatin alterations in H3K27M pediatric glioma
Science Advances, Oct 31, 2018; DOI: 10.1126/sciadv.aau5935
In-vitro stimulation of epithelial cells
Authors: Shahanshah Khan and Hasan Zaki
Date: Dec 16, 2022，Views：991
Original protocol used in research article：
SARS-CoV-2 spike protein induces inflammation via TLR2-dependent activation of the NF-κB pathway
eLife, Dec 6, 2021; DOI: 10.7554/eLife.68563
Plasmid nucleofection
Authors: Dawei Sun and Emma L Rawlins
Date: Dec 14, 2022，Views：1493
Original protocol used in research article：
A functional genetic toolbox for human tissue-derived organoids
eLife, Oct 6, 2021; DOI: 10.7554/eLife.67886
Lipofectamine transfection of organoids
Authors: Dawei Sun and Emma L Rawlins
Date: Dec 14, 2022，Views：2216
Original protocol used in research article：
A functional genetic toolbox for human tissue-derived organoids
eLife, Oct 6, 2021; DOI: 10.7554/eLife.67886
The Intolerance of Uncertainty Scale – Short Form (IUS-12)
Authors: Einav Gozansky and Hadas Okon-Singer
Date: Dec 14, 2022，Views：3200
Original protocol used in research article：
Identifying Variables That Predict Depression Following the General Lockdown During the COVID-19 Pandemic
Frontiers in Psychology, May 17, 2021; DOI: 10.3389/fpsyg.2021.680768
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