Protocols shared by authors via RaP service
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Monocyte isolation, macrophage differentiation, and OC generation
Authors: Qingqing Wang, Shunwu Fan, Xin Liu and Xianfeng Lin
Date: Dec 28, 2022，Views：706
Original protocol used in research article：
Suppression of osteoclast multinucleation via a posttranscriptional regulation–based spatiotemporally selective delivery system
Science Advances, Jun 29, 2022; DOI: 10.1126/sciadv.abn3333
Overview of the prediction pipeline
Authors: Diego del Alamo, Davide Sala, Hassane S Mchaourab and Jens Meiler
Date: Dec 27, 2022，Views：858
Original protocol used in research article：
Sampling alternative conformational states of transporters and receptors with AlphaFold2
eLife, Mar 3, 2022; DOI: 10.7554/eLife.75751
Model building and refinement
Authors: Qiang Su and Yigong Shi
Date: Dec 25, 2022，Views：704
Original protocol used in research article：
Cryo-EM structure of the human IgM B cell receptor
Science, Aug 18, 2022; DOI: 10.1126/science.abo3923
S-specific immunoglobulin ELISA
Authors: Karen Colwill, Reuben Samson, Queenie Hu, Anne-Claude Gingras, Natalia Martin Orozco, Jun Liu, Eric G. Marcusson and Mario A. Ostrowski
Date: Dec 23, 2022，Views：713
Original protocol used in research article：
Preclinical evaluation of a SARS-CoV-2 mRNA vaccine PTX-COVID19-B
Science Advances, Jan 19, 2022; DOI: 10.1126/sciadv.abj9815
par-seqFISH
Authors: Daniel Dar, Long Cai and Dianne K. Newman
Date: Dec 20, 2022，Views：766
Original protocol used in research article：
Spatial transcriptomics of planktonic and sessile bacterial populations at single-cell resolution
Science, Aug 12, 2021; DOI: 10.1126/science.abi4882
Real-time quantitative PCR (qRT-PCR)
Authors: Gail Mandel and Tamilla Nechiporuk
Date: Dec 18, 2022，Views：836
Original protocol used in research article：
The REST remodeling complex protects genomic integrity during embryonic neurogenesis
eLife, Jan 8, 2016; DOI: 10.7554/eLife.09584
Wire hang test
Authors: Michael A Petr and Rafael de Cabo
Date: Dec 18, 2022，Views：1391
Original protocol used in research article：
A cross-sectional study of functional and metabolic changes during aging through the lifespan in male mice
eLife, Apr 20, 2021; DOI: 10.7554/eLife.62952
HMT assay
Authors: Danny Reinberg|| and Chul-Hwan Lee
Date: Dec 16, 2022，Views：776
Original protocol used in research article：
Multiple modes of PRC2 inhibition elicit global chromatin alterations in H3K27M pediatric glioma
Science Advances, Oct 31, 2018; DOI: 10.1126/sciadv.aau5935
In-vitro stimulation of epithelial cells
Authors: Shahanshah Khan and Hasan Zaki
Date: Dec 16, 2022，Views：800
Original protocol used in research article：
SARS-CoV-2 spike protein induces inflammation via TLR2-dependent activation of the NF-κB pathway
eLife, Dec 6, 2021; DOI: 10.7554/eLife.68563
Plasmid nucleofection
Authors: Dawei Sun and Emma L Rawlins
Date: Dec 14, 2022，Views：1023
Original protocol used in research article：
A functional genetic toolbox for human tissue-derived organoids
eLife, Oct 6, 2021; DOI: 10.7554/eLife.67886
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