Protocols shared by authors via RaP service
Journal
Stereotactic injections of AAV in hippocampal CA1
Authors: Fujun Luo and Alessandra Sclip
Date: Jan 6, 2023，Views：636
Original protocol used in research article：
Neurexins regulate presynaptic GABAB-receptors at central synapses
Nature Communications, Apr 22, 2021; DOI: 10.1038/s41467-021-22753-5
Gastrointestinal Symptom Rating Scale-IBS
Author: Melissa Hunt
Date: Jan 4, 2023，Views：754
Original protocol used in research article：
Efficacy of Zemedy, a Mobile Digital Therapeutic for the Self-management of Irritable Bowel Syndrome: Crossover Randomized Controlled Trial
JMIR mHealth and uHealth, May 20, 2021; DOI: 10.2196/26152
siRNA transfection in Calu-3 cells
Authors: Laura Martin-Sancho and Sumit K. Chanda
Date: Jan 4, 2023，Views：773
Original protocol used in research article：
Functional landscape of SARS-CoV-2 cellular restriction
Mol Cell, Apr 13, 2021; DOI: 10.1016/j.molcel.2021.04.008
Immunofluorescence of Drosophila tissues
Author: Julia B Cordero
Date: Jan 4, 2023，Views：663
Original protocol used in research article：
RAL GTPases mediate EGFR-driven intestinal stem cell proliferation and tumourigenesis
eLife, Jun 7, 2021; DOI: 10.7554/eLife.63807
Histological assessment of adipocyte size, non‐alcoholic fatty liver disease and villi structure
Authors: Natalie J Haywood and Mark T Kearney
Date: Jan 3, 2023，Views：665
Original protocol used in research article：
Endothelial IGF‐1 receptor mediates crosstalk with the gut wall to regulate microbiota in obesity
EMBO Reports, May 2, 2021; DOI: 10.15252/embr.202050767
Clinical Study design
Author: Heidi H. Kong
Date: Jan 3, 2023，Views：586
Original protocol used in research article：
Alterations of human skin microbiome and expansion of antimicrobial resistance after systemic antibiotics
Science Translational Medicine, Dec 22, 2021; DOI: 10.1126/scitranslmed.abd8077
Apoptosis induction
Authors: Britt A Glaunsinger and Christopher Duncan-Lewis
Date: Jan 2, 2023，Views：599
Original protocol used in research article：
Cytoplasmic mRNA decay represses RNA polymerase II transcription during early apoptosis
eLife, Jun 4, 2021; DOI: 10.7554/eLife.58342
Lentivirus packaging
Author: Jakub Gemperle
Date: Jan 2, 2023，Views：647
Original protocol used in research article：
On demand expression control of endogenous genes with DExCon, DExogron and LUXon reveals differential dynamics of Rab11 family members
eLife, ; DOI: 10.7554/eLife.76651
20-hydroxyecdysone (20E) feeding of Drosophila larvae
Authors: Jacob S Jaszczak, Laura DeVault, Lily Jan and Yuh Nung Jan
Date: Dec 28, 2022，Views：701
Original protocol used in research article：
Steroid hormone signaling activates thermal nociception during Drosophila peripheral nervous system development
eLife, Mar 30, 2022; DOI: 10.7554/eLife.76464
Monocyte isolation, macrophage differentiation, and OC generation
Authors: Qingqing Wang, Shunwu Fan, Xin Liu and Xianfeng Lin
Date: Dec 28, 2022，Views：706
Original protocol used in research article：
Suppression of osteoclast multinucleation via a posttranscriptional regulation–based spatiotemporally selective delivery system
Science Advances, Jun 29, 2022; DOI: 10.1126/sciadv.abn3333
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