Bio-101 - Improve Research Reproducibility

MAG Bead DNA Extraction Protocol

Authors: Tamara D. Simon and Christopher E. Pope
Date: Jul 11, 2023，Views：714

Original protocol used in research article：

Characterization of cerebrospinal fluid (CSF) microbiota from patients with CSF shunt infection and reinfection using high throughput sequencing of 16S ribosomal RNAgenes
PLoS ONE, Jan 6, 2021; DOI: 10.1371/journal.pone.0244643

CyTOF analysis

Author: Chu-Xia Deng
Date: Jul 11, 2023，Views：708

Original protocol used in research article：

Cullin-5 deficiency orchestrates the tumor microenvironment to promote mammary tumor development through CREB1-CCL2 signaling
Science Advances, Jan 20, 2023; DOI: 10.1126/sciadv.abq1395

Analysis of differential expression in tumor and matched normal tissues

Author: Ying Yuan
Date: Jul 1, 2023，Views：743

Original protocol used in research article：

Pan-cancer analyses demonstrate that ANKRD6 is associated with a poor prognosis and correlates with M2 macrophage infiltration in colon cancer
Chinese Journal of Cancer Research, Feb 28, 2021; DOI: 10.21147/j.issn.1000-9604.2021.01.10

Immunofluorescence, immunoprecipitation, and ELISA using the Scicons anti-dsRNA J2 antibody

Authors: Elizabeth Ochoa and Bess Frost
Date: Jun 22, 2023，Views：949

Original protocol used in research article：

Pathogenic tau–induced transposable element–derived dsRNA drives neuroinflammation
Science Advances, Jan 6, 2023; DOI: 10.1126/sciadv.abq5423

APEX2 biotinylation in brain slices

Authors: David Sulzer and Peter A Sims
Date: Jun 21, 2023，Views：899

Original protocol used in research article：

Subcellular proteomics of dopamine neurons in the mouse brain
eLife, Jan 31, 2022; DOI: 10.7554/eLife.70921

CUT&RUN library preparation

Author: Paolo Emanuele Forni
Date: Jun 20, 2023，Views：29

Original protocol used in research article：

Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2ε in vomeronasal sensory neurons
eLife, ; DOI: 10.7554/eLife.77259

Sortase A conjugation of HIV-1 envelope trimers to ferritin nanoparticles

Author: Kevin O. Saunders
Date: Jun 13, 2023，Views：905

Original protocol used in research article：

Targeted selection of HIV-specific antibody mutations by engineering B cell maturation
Science, Dec 5, 2019; DOI: 10.1126/science.aay7199

crystal fish imaging

Authors: Francois Kroll and Jason Rihel
Date: Jun 13, 2023，Views：858

Original protocol used in research article：

A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
eLife, Jan 8, 2021; DOI: 10.7554/eLife.59683

2.3.5. The Visual Analog Scale for Irritable Bowel Syndrome Questionnaire

Authors: Clara Nilholm and Bodil Ohlsson
Date: Jun 11, 2023，Views：7

Original protocol used in research article：

Assessment of a 4-Week Starch- and Sucrose-Reduced Diet and Its Effects on Gastrointestinal Symptoms and Inflammatory Parameters among Patients with Irritable Bowel Syndrome
Nutrients, Jan 28, 2021; DOI: 10.3390/nu13020416

CUMS model

Authors: Shu Yan Yu, Ye Li, Cuiqin Fan and Changmin Wang
Date: Jun 11, 2023，Views：954

Original protocol used in research article：

Stress-induced reduction of Na+/H+ exchanger isoform 1 promotes maladaptation of neuroplasticity and exacerbates depressive behaviors
Science Advances, Nov 11, 2022; DOI: 10.1126/sciadv.add7063

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