Bio-101 - Improve Research Reproducibility

Ex vivo muscle function testing

Authors: Jeffrey Molendijk, Ronnie Blazev and Benjamin L Parker
Date: May 14, 2024，Views：500

Original protocol used in research article：

Proteome-wide systems genetics identifies UFMylation as a regulator of skeletal muscle function
eLife, Dec 6, 2022; DOI: 10.7554/eLife.82951

Histology and immunofluorescence staining and analysis

Author: Derek C Sung
Date: May 13, 2024，Views：527

Original protocol used in research article：

VE-cadherin enables trophoblast endovascular invasion and spiral artery remodeling during placental development
eLife, Apr 29, 2022; DOI: 10.7554/eLife.77241

Culture of naïve and primed hPSCs

Author: Thorold W Theunissen
Date: May 8, 2024，Views：23

Original protocol used in research article：

Derivation of trophoblast stem cells from naïve human pluripotent stem cells
eLife, Feb 12, 2020; DOI: 10.7554/eLife.52504

Plasmids preparation and lentiviral production

Authors: Albert H. Kim, Diane Mao and Rowland Han
Date: May 8, 2024，Views：583

Original protocol used in research article：

TREM2 inhibition triggers antitumor cell activity of myeloid cells in glioblastoma
Science Advances, May 12, 2023; DOI: 10.1126/sciadv.ade3559

4.2. Arabidopsis Genotypes Growth Conditions and Salt Stress Induction

Authors: Nkulu Kabange Rolly and Byung-Wook Yun
Date: May 7, 2024，Views：268

Original protocol used in research article：

Salinity Stress-Mediated Suppression of Expression of Salt Overly Sensitive Signaling Pathway Genes Suggests Negative Regulation by AtbZIP62 Transcription Factor in Arabidopsis thaliana
International Journal of Molecular Sciences, Mar 3, 2020; DOI: 10.3390/ijms21051726

Luciferase reporter assay

Authors: Takaki Asano, Bertrand Boisson and Jean-Laurent Casanova
Date: May 7, 2024，Views：575

Original protocol used in research article：

X-linked recessive TLR7 deficiency in ~1% of men under 60 years old with life-threatening COVID-19
Science Immunology, Aug 19, 2021; DOI: 10.1126/sciimmunol.abl4348

Brain virus injection and optic fiber implantation for unraveling M1 layer-specific pathways for pain relief

Authors: Zheng Gan and Rohini Kuner
Date: May 5, 2024，Views：637

Original protocol used in research article：

Layer-specific pain relief pathways originating from primary motor cortex
Science, Dec 22, 2022; DOI: 10.1126/science.add4391

Construction of an E. coli strain (W3110) harboring chromosomal 3xFLAG-tagged RutR

Authors: Shun Tu and Sheng-Ce Tao
Date: May 2, 2024，Views：580

Original protocol used in research article：

YcgC represents a new protein deacetylase family in prokaryotes
eLife, Dec 30, 2015; DOI: 10.7554/eLife.05322

Control participants in the online frame rating task

Authors: Efe Soyman, Christian Keysers and Valeria Gazzola
Date: May 1, 2024，Views：52

Original protocol used in research article：

Intracranial human recordings reveal association between neural activity and perceived intensity for the pain of others in the insula
eLife, Nov 3, 2022; DOI: 10.7554/eLife.75197

B cell magnetic fractionation

Authors: Silvia Portugal and Peter D Crompton
Date: May 1, 2024，Views：27

Original protocol used in research article：

Malaria-associated atypical memory B cells exhibit markedly reduced B cell receptor signaling and effector function
eLife, May 8, 2015; DOI: 10.7554/eLife.07218

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