Protocols shared by authors via RaP service
Journal
Cell clustering and classification
Author: James YH Li
Date: Aug 27, 2021，Views：785
Original protocol used in research article：
Specification of diverse cell types during early neurogenesis of the mouse cerebellum
eLife, Feb 8, 2019; DOI: 10.7554/eLife.42388
Track moving cells in 3D time lapse images by using 3DeeCellTracker
Authors: Chentao Wen and Koutarou D Kimura
Date: Aug 24, 2021，Views：1069
Original protocol used in research article：
3DeeCellTracker, a deep learning-based pipeline for segmenting and tracking cells in 3D time lapse images
eLife, Mar 30, 2021; DOI: 10.7554/eLife.59187
Colocalization analysis for immunostained zebrafish cryosections with or without label retention expansion microscopy (LR-ExM) by JACoP
Authors: Xiang Zhao and Su Guo
Date: Aug 11, 2021，Views：971
Original protocol used in research article：
Polarized endosome dynamics engage cytoplasmic Par-3 that recruits dynein during asymmetric cell division
Science Advances, Jun 11, 2021; DOI: 10.1126/sciadv.abg1244
Sample preparation TMT LC-MS3 mass spectrometry
Authors: Katherine A Donovan and Eric S Fischer
Date: Aug 5, 2021，Views：979
Original protocol used in research article：
Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome
eLife, Aug 1, 2018; DOI: 10.7554/eLife.38430
Eicosanoid and cytokine analysis
Authors: Antonie Lechner and Julia Esser-von Bieren
Date: Aug 2, 2021，Views：752
Original protocol used in research article：
An anti-inflammatory eicosanoid switch mediates the suppression of type-2 inflammation by helminth larval products
Science Translational Medicine, Apr 21, 2020; DOI: 10.1126/scitranslmed.aay0605
Comet Assay for DNA Damage
Author: Jianmin Wan
Date: Jul 30, 2021，Views：843
Original protocol used in research article：
Earlier Degraded Tapetum1 (EDT1) Encodes an ATP-Citrate Lyase Required for Tapetum Programmed Cell Death1,[OPEN]
Plant Physiology, Sep 12, 2019; DOI: 10.1104/pp.19.00202
Image processing and data analysis
Authors: Daria Amiad-Pavlov, Dana Lorber and Talila Volk
Date: Jul 28, 2021，Views：830
Original protocol used in research article：
Live imaging of chromatin distribution reveals novel principles of nuclear architecture and chromatin compartmentalization
Science Advances, Jun 2, 2021; DOI: 10.1126/sciadv.abf6251
Video acquisition and CASA analysis of mouse sperm
Authors: Pei-Hsuan Wu, Zhiping Weng and Phillip D. Zamore
Date: Jul 27, 2021，Views：766
Original protocol used in research article：
The Evolutionarily Conserved piRNA-producing Locus pi6 Is Required for Male Mouse Fertility
Nat Genet, Jun 29, 2020; DOI: 10.1038/s41588-020-0657-7
Bacterial colonization in vivo
Authors: Kai Yang and Zhuang Liu
Date: Jul 26, 2021，Views：980
Original protocol used in research article：
Bacteria-triggered tumor-specific thrombosis to enable potent photothermal immunotherapy of cancer
Science Advances, Aug 13, 2020; DOI: 10.1126/sciadv.aba3546
Virus-like-particle production and viral entry assays.
Authors: Shirley Qiu, Alexandra Phan, Kathy Fu and Marceline Côté
Date: Jul 23, 2021，Views：815
Original protocol used in research article：
Filoviruses Use the HOPS Complex and UVRAG To Traffic to Niemann-Pick C1 Compartments during Viral Entry
Journal of Virology, Jan 1, 2020; DOI: 10.1128/JVI.01002-20
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