Protocols shared by authors via RaP service
Journal
Single-cell RNA-seq (scRNA-seq) Methanol fixation
Author: Thanh Hoang
Date: Feb 21, 2022，Views：667
Original protocol used in research article：
Gene regulatory networks controlling vertebrate retinal regeneration
Science, Oct 1, 2020; DOI: 10.1126/science.abb8598
SMP isolation and culture in osteogenic media
Authors: Qian Cong and Yingzi Yang
Date: Feb 18, 2022，Views：665
Original protocol used in research article：
A self-amplifying loop of YAP and SHH drives formation and expansion of heterotopic ossification
Science Translational Medicine, Jun 23, 2021; DOI: 10.1126/scitranslmed.abb2233
Western blotting
Author: Akihiko Yokoyama
Date: Feb 13, 2022，Views：832
Original protocol used in research article：
HOXA9 promotes MYC-mediated leukemogenesis by maintaining gene expression for multiple anti-apoptotic pathways
eLife, Jul 26, 2021; DOI: 10.7554/eLife.64148
Histology
Authors: Nichola N Barron and David A Bechtold
Date: Feb 12, 2022，Views：894
Original protocol used in research article：
Adipocyte NR1D1 dictates adipose tissue expansion during obesity
eLife, Aug 5, 2021; DOI: 10.7554/eLife.63324
IIH6 Assays
Authors: Ameya Walimbe and Kevin P Campbell
Date: Feb 10, 2022，Views：776
Original protocol used in research article：
The functional O-mannose glycan on α-dystroglycan contains a phospho-ribitol primed for matriglycan addition
eLife, Apr 29, 2016; DOI: 10.7554/eLife.14473
Radiochemistry of [11C]MK-6884 and [3H]MK-6884
Author: Wenping Li
Date: Feb 7, 2022，Views：764
Original protocol used in research article：
The PET tracer [11C]MK-6884 quantifies M4 muscarinic receptor in rhesus monkeys and patients with Alzheimer’s disease
Science Translational Medicine, Jan 12, 2022; DOI: 10.1126/scitranslmed.abg3684
Spatial analysis of cell clusters
Authors: Nicholas JP Ryba and Lars J von Buchholtz
Date: Feb 7, 2022，Views：710
Original protocol used in research article：
Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons
eLife, Nov 26, 2021; DOI: 10.7554/eLife.71752
Phagocytosis assays
Author: Rasmus Berglund
Date: Feb 7, 2022，Views：700
Original protocol used in research article：
Microglial autophagy–associated phagocytosis is essential for recovery from neuroinflammation
Science Immunology, Oct 15, 2020; DOI: 10.1126/sciimmunol.abb5077
RNA-Seq
Authors: Prashant Rajbhandari and Peter Tontonoz
Date: Feb 4, 2022，Views：761
Original protocol used in research article：
Single cell analysis reveals immune cell–adipocyte crosstalk regulating the transcription of thermogenic adipocytes
eLife, Oct 23, 2019; DOI: 10.7554/eLife.49501
Antibodies
Authors: Ji Eun Oh and Akiko Iwasaki
Date: Feb 2, 2022，Views：695
Original protocol used in research article：
Intranasal priming induces local lung-resident B cell populations that secrete protective mucosal antiviral IgA
Science Immunology, Dec 10, 2021; DOI: 10.1126/sciimmunol.abj5129
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