Protocols shared by authors via RaP service
Journal
Expression and preparation of proteins
Authors: Tao Xie, Tamjeed Saleh, Paolo Rossi and Charalampos G. Kalodimos
Date: Mar 21, 2022，Views：670
Original protocol used in research article：
Conformational states dynamically populated by a kinase determine its function
Science, Oct 8, 2020; DOI: 10.1126/science.abc2754
Preparation for two-photon calcium imaging in visual cortex
Authors: Carsen Stringer, Marius Pachitariu and Kenneth D. Harris
Date: Mar 18, 2022，Views：613
Original protocol used in research article：
Spontaneous behaviors drive multidimensional, brainwide activity
Science, Apr 19, 2019; DOI: 10.1126/science.aav7893
Generation of erythroid-specific TLR9-deficient mice
Authors: LK Metthew Lam, Kaitlyn Eckart and Nilam S. Mangalmurti
Date: Mar 17, 2022，Views：731
Original protocol used in research article：
DNA binding to TLR9 expressed by red blood cells promotes innate immune activation and anemia
Science Translational Medicine, Oct 20, 2021; DOI: 10.1126/scitranslmed.abj1008
Rabbit bioassay of in vivo insulin glargine activity
Authors: Parag Goyal and Ramakrishnan Melarkode
Date: Mar 17, 2022，Views：589
Original protocol used in research article：
Physicochemical and functional characterization of MYL-1501D, a proposed biosimilar to insulin glargine
PLoS ONE, Jun 16, 2021; DOI: 10.1371/journal.pone.0253168
αCD45 infusions
Authors: E. Lake Potter and Mario Roederer
Date: Mar 16, 2022，Views：670
Original protocol used in research article：
Measurement of leukocyte trafficking kinetics in macaques by serial intravascular staining
Science Translational Medicine, Jan 13, 2021; DOI: 10.1126/scitranslmed.abb4582
Nile red membrane staining
Author: Ronit Vogt Sionov
Date: Mar 16, 2022，Views：889
Original protocol used in research article：
Anandamide alters the membrane properties, halts the cell division and prevents drug efflux in multidrug resistant Staphylococcus aureus
Scientific Reports, Apr 22, 2021; DOI: 10.1038/s41598-021-88099-6
LRP10 CRISPR/Cas9-mediated knockout in HuTu 80 cells
Authors: Martyna Grochowska and Wim Mandemakers
Date: Mar 14, 2022，Views：676
Original protocol used in research article：
LRP10 interacts with SORL1 in the intracellular vesicle trafficking pathway in non-neuronal brain cells and localises to Lewy bodies in Parkinson’s disease and dementia with Lewy bodies
Acta Neuropathol, Apr 28, 2021; DOI: 10.1007/s00401-021-02313-3
Differential abundance analysis of proteomics data
Authors: Sven Schenk, Stephanie C Bannister, Florian Raible and Kristin Tessmar-Raible
Date: Mar 11, 2022，Views：825
Original protocol used in research article：
Combined transcriptome and proteome profiling reveals specific molecular brain signatures for sex, maturation and circalunar clock phase
eLife, Feb 15, 2019; DOI: 10.7554/eLife.41556
Antibiotic treatment and fecal microbiota transplantation
Authors: Ping Li, Jing Li and E-Hu Liu
Date: Mar 10, 2022，Views：694
Original protocol used in research article：
Citrus polymethoxyflavones attenuate metabolic syndrome by regulating gut microbiome and amino acid metabolism
Science Advances, Jan 3, 2020; DOI: 10.1126/sciadv.aax6208
Isolation of lymphocytes from mouse skin
Authors: Joshua M. Moreau and Michael D. Rosenblum
Date: Mar 10, 2022，Views：842
Original protocol used in research article：
Regulatory T cells promote innate inflammation after skin barrier breach via TGF-β activation
Science Immunology, Aug 26, 2021; DOI: 10.1126/sciimmunol.abg2329
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