Protocols shared by authors via RaP service
Journal
Single-cell TCR sequencing
Authors: Daniel T. Leung and Owen Jensen
Date: Apr 22, 2022，Views：626
Original protocol used in research article：
A subset of follicular helper-like MAIT cells can provide B cell help and support antibody production in the mucosa
Science Immunology, Jan 14, 2022; DOI: 10.1126/sciimmunol.abe8931
mAb competition assay
Authors: Tomas Strandin, Andrew S. Herbert, Mattias N. E. Forsell, Laura M. Walker and Kartik Chandran
Date: Apr 21, 2022，Views：577
Original protocol used in research article：
Human antibody recognizing a quaternary epitope in the Puumala virus glycoprotein provides broad protection against orthohantaviruses
Science Translational Medicine, Mar 16, 2022; DOI: 10.1126/scitranslmed.abl5399
Differential gene expression analysis
Authors: Wei Xie, Xiaotong Wu and Hongmei Zhang
Date: Apr 21, 2022，Views：632
Original protocol used in research article：
Methylome inheritance and enhancer dememorization reset an epigenetic gate safeguarding embryonic programs
Science Advances, Dec 22, 2021; DOI: 10.1126/sciadv.abl3858
Statistical analysis
Authors: Ramona A.J. Zwamborn and Jan H. Veldink
Date: Apr 20, 2022，Views：607
Original protocol used in research article：
Genome-wide study of DNA methylation shows alterations in metabolic, inflammatory, and cholesterol pathways in ALS
Science Translational Medicine, Feb 23, 2022; DOI: 10.1126/scitranslmed.abj0264
Induction of rEpiLCs and rPGCLCs
Authors: Mami Oikawa, Masumi Hirabayashi and Toshihiro Kobayashi
Date: Apr 19, 2022，Views：624
Original protocol used in research article：
Functional primordial germ cell–like cells from pluripotent stem cells in rats
Science, Apr 7, 2022; DOI: 10.1126/science.abl4412
scRNA-seq data processing
Authors: Wei Xie, Xiaotong Wu and Hongmei Zhang
Date: Apr 18, 2022，Views：613
Original protocol used in research article：
Methylome inheritance and enhancer dememorization reset an epigenetic gate safeguarding embryonic programs
Science Advances, Dec 22, 2021; DOI: 10.1126/sciadv.abl3858
Checkerboard Method
Authors: Ping Huang and Yifei Xu
Date: Apr 18, 2022，Views：630
Original protocol used in research article：
Unraveling the Novel Effect of Patchouli Alcohol Against the Antibiotic Resistance of Helicobacter pylori
Frontiers in Microbiology, Jun 2, 2021; DOI: 10.3389/fmicb.2021.674560
Mouse models
Authors: Xiaofei Chen, Xiao Chen and Jiacan Su
Date: Apr 18, 2022，Views：565
Original protocol used in research article：
Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss
Science Advances, Nov 18, 2020; DOI: 10.1126/sciadv.abb7135
In vitro phosphorylation assay and LC-MS/MS
Authors: Lin Wang, Noe Perron, Bowen Tan, Craig P. Dufresne, Sixue chen and Xiuren Zhang
Date: Apr 14, 2022，Views：677
Original protocol used in research article：
PRP4KA phosphorylates SERRATE for degradation via 20S proteasome to fine-tune miRNA production in Arabidopsis
Science Advances, Mar 25, 2022; DOI: 10.1126/sciadv.abm8435
In vitro polysaccharide precipitation assays and fungal protection assays against chitinases
Authors: Ioannis Stergiopoulos and Li-Hung Chen
Date: Apr 13, 2022，Views：605
Original protocol used in research article：
A diverse member of the fungal Avr4 effector family interacts with de-esterified pectin in plant cell walls to disrupt their integrity
Science Advances, May 7, 2021; DOI: 10.1126/sciadv.abe0809
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