Protocols shared by authors via RaP service
Journal
m6A-RNA immunoprecipitation (MeRIP) and MeRIP-qPCR
Authors: Zhigang Hu and Zhigang Guo
Date: Oct 24, 2022，Views：732
Original protocol used in research article：
METTL3 promotes homologous recombination repair and modulates chemotherapeutic response in breast cancer by regulating the EGF/RAD51 axis
eLife, May 3, 2022; DOI: 10.7554/eLife.75231
Mass spectrometry sample preparation
Authors: Tonia L Vincent and Yoanna Ariosa-Morejon
Date: Oct 23, 2022，Views：456
Original protocol used in research article：
Age-dependent changes in protein incorporation into collagen-rich tissues of mice by in vivo pulsed SILAC labelling
eLife, Sep 28, 2021; DOI: 10.7554/eLife.66635
Hydroxyl radical-mediated cleavage of nucleosomal DNA
Authors: George A. Kassavetis, Grisel Cruz-Becerra and James Takuro Kadonaga
Date: Oct 22, 2022，Views：645
Original protocol used in research article：
The tardigrade damage suppressor protein binds to nucleosomes and protects DNA from hydroxyl radicals
eLife, Oct 1, 2019; DOI: 10.7554/eLife.47682
Optogenetic inhibition/activation of ERαvlVMH → DRN neurons
Authors: Pingwen Xu and Yong Xu
Date: Oct 21, 2022，Views：418
Original protocol used in research article：
An estrogen-sensitive hypothalamus-midbrain neural circuit controls thermogenesis and physical activity
Science Advances, Jan 19, 2022; DOI: 10.1126/sciadv.abk0185
ELISA assessment of recombinant salivary proteins
Authors: Jaqueline Matias, Gunjan Arora and Erol Fikrig
Date: Oct 21, 2022，Views：463
Original protocol used in research article：
mRNA vaccination induces tick resistance and prevents transmission of the Lyme disease agent
Science Translational Medicine, Nov 17, 2021; DOI: 10.1126/scitranslmed.abj9827
Emotion Regulation Questionnaire
Authors: Mohsen Dehghani and Ali Khatibi
Date: Oct 20, 2022，Views：408
Original protocol used in research article：
Further Support for the Psychometric Properties of the Farsi Version of Perth Alexithymia Questionnaire
Frontiers in Psychology, Apr 14, 2021; DOI: 10.3389/fpsyg.2021.657660
Sequence alignment
Author: Acacia F. Dishman
Date: Oct 19, 2022，Views：400
Original protocol used in research article：
Evolution of fold switching in a metamorphic protein
Science, Jan 1, 2021; DOI: 10.1126/science.abd8700
Acoustic stimuli
Author: Philippe Albouy
Date: Oct 19, 2022，Views：467
Original protocol used in research article：
Distinct sensitivity to spectrotemporal modulation supports brain asymmetry for speech and melody
Science, Feb 28, 2020; DOI: 10.1126/science.aaz3468
Cell Culture and Drug Treatment
Authors: Na Huang and Wei Zhao
Date: Oct 19, 2022，Views：453
Original protocol used in research article：
Deoxypodophyllotoxin Inhibits Non-Small Cell Lung Cancer Cell Growth by Reducing HIF-1α-Mediated Glycolysis
Frontiers in Oncology, Feb 24, 2021; DOI: 10.3389/fonc.2021.629543
3.3.3. Pancreatic Lipase Inhibition Assay
Authors: Vítor Spínola and Paula C. Castilho
Date: Oct 19, 2022，Views：508
Original protocol used in research article：
Assessing the In Vitro Inhibitory Effects on Key Enzymes Linked to Type-2 Diabetes and Obesity and Protein Glycation by Phenolic Compounds of Lauraceae Plant Species Endemic to the Laurisilva Forest
Molecules, Apr 1, 2021; DOI: 10.3390/molecules26072023
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