Protocols shared by authors via RaP service
Journal
Echo-MRI analysis
Authors: Rozalyn M Anderson and Priya Balasubramanian
Date: Aug 31, 2022，Views：593
Original protocol used in research article：
Adiponectin receptor agonist AdipoRon improves skeletal muscle function in aged mice
eLife, Mar 17, 2022; DOI: 10.7554/eLife.71282
APC maturation evaluation
Authors: Mariusz Skwarczynski and Istvan Toth
Date: Aug 30, 2022，Views：552
Original protocol used in research article：
Poly(amino acids) as a potent self-adjuvanting delivery system for peptide-based nanovaccines
Science Advances, Jan 28, 2020; DOI: 10.1126/sciadv.aax2285
Immunoblot analysis
Authors: Sandra Olenic and Lee Kroos
Date: Aug 30, 2022，Views：536
Original protocol used in research article：
Inhibitory proteins block substrate access by occupying the active site cleft of Bacillus subtilis intramembrane protease SpoIVFB
eLife, ; DOI: 10.7554/eLife.74275
Enzyme-linked immunosorbent assay (ELISA)
Authors: Maria E. Diaz-Ortiz and Alice S. Chen-Plotkin
Date: Aug 30, 2022，Views：540
Original protocol used in research article：
GPNMB confers risk for Parkinson’s disease through interaction with α-synuclein
Science, Aug 19, 2022; DOI: 10.1126/science.abk0637
Immunofluorescence
Authors: Francesco M Piccolo and Ali H Brivanlou
Date: Aug 30, 2022，Views：526
Original protocol used in research article：
Role of YAP in early ectodermal specification and a Huntington's Disease model of human neurulation
eLife, ; DOI: 10.7554/eLife.73075
Quantitative RT-PCR
Authors: Zhihui Liu and Thomas C Südhof
Date: Aug 29, 2022，Views：574
Original protocol used in research article：
Deletion of Calsyntenin-3, an atypical cadherin, suppresses inhibitory synapses but increases excitatory parallel-fiber synapses in cerebellum
eLife, Apr 14, 2022; DOI: 10.7554/eLife.70664
Inference of copy number variations and clonality analysis.
Authors: Xiyuan Zhang and Angela C. Hirbe
Date: Aug 29, 2022，Views：776
Original protocol used in research article：
Chromosome 8 gain is associated with high-grade transformation in MPNST
JCI Insight, Mar 22, 2021; DOI: 10.1172/jci.insight.146351
Triglycerides and glycogen quantifications
Authors: Katarina Akhmetova and Igor Chesnokov
Date: Aug 29, 2022，Views：548
Original protocol used in research article：
Drosophila STING protein has a role in lipid metabolism
eLife, Sep 1, 2021; DOI: 10.7554/eLife.67358
Immunostaining
Authors: Amanda C Ignacz and Clarissa A Henry
Date: Aug 29, 2022，Views：556
Original protocol used in research article：
Beneficial impacts of neuromuscular electrical stimulation on muscle structure and function in the zebrafish model of Duchenne muscular dystrophy
eLife, ; DOI: 10.7554/eLife.62760
Luciferase assay
Authors: Jochen Winterer and Gerhard Schratt
Date: Aug 29, 2022，Views：552
Original protocol used in research article：
MicroRNA-138 controls hippocampal interneuron function and short-term memory in mice
eLife, Mar 15, 2022; DOI: 10.7554/eLife.74056
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