Protocols shared by authors via RaP service
Journal
Fabrication of the DOAMS
Authors: Wei Chen and Giovanni Traverso
Date: Sep 8, 2022，Views：550
Original protocol used in research article：
Dynamic omnidirectional adhesive microneedle system for oral macromolecular drug delivery
Science Advances, Jan 5, 2022; DOI: 10.1126/sciadv.abk1792
Unsupervised clustering of 4,000-gene human MERFISH data
Authors: Rongxin Fang and Xiaowei Zhuang
Date: Sep 8, 2022，Views：558
Original protocol used in research article：
Conservation and divergence of cortical cell organization in human and mouse revealed by MERFISH
Science, Jun 30, 2022; DOI: 10.1126/science.abm1741
Measurement of vaccine-induced IgA antibody mucosal samples
Authors: Stephanie N. Langel and Sean N. Tucker
Date: Sep 8, 2022，Views：595
Original protocol used in research article：
Adenovirus type 5 SARS-CoV-2 vaccines delivered orally or intranasally reduced disease severity and transmission in a hamster model
Science Translational Medicine, May 5, 2022; DOI: 10.1126/scitranslmed.abn6868
RT-qPCR
Authors: Colin J Dinsmore and Philippe Soriano
Date: Sep 8, 2022，Views：775
Original protocol used in research article：
Differential regulation of cranial and cardiac neural crest by serum response factor and its cofactors
eLife, Jan 19, 2022; DOI: 10.7554/eLife.75106
Detection of SARS-CoV-2 genomic RNA in nasal swabs and lung homogenates by quantitative reverse transcription polymerase chain reaction
Authors: Stephanie N. Langel and Sean N. Tucker
Date: Sep 8, 2022，Views：552
Original protocol used in research article：
Adenovirus type 5 SARS-CoV-2 vaccines delivered orally or intranasally reduced disease severity and transmission in a hamster model
Science Translational Medicine, May 5, 2022; DOI: 10.1126/scitranslmed.abn6868
Fly stocks and food
Authors: Colin D McClure and Tony D Southall
Date: Sep 8, 2022，Views：748
Original protocol used in research article：
An auxin-inducible, GAL4-compatible, gene expression system for Drosophila
eLife, ; DOI: 10.7554/eLife.67598
Yeast toxicity assays
Authors: Zhao-Qing Luo and Jiaqi Fu
Date: Sep 8, 2022，Views：564
Original protocol used in research article：
Legionella pneumophila modulates host energy metabolism by ADP-ribosylation of ADP/ATP translocases
eLife, Jan 27, 2022; DOI: 10.7554/eLife.73611
Crystal violet assay
Authors: Lisanne de Vor and Suzan HM Rooijakkers
Date: Sep 8, 2022，Views：1463
Original protocol used in research article：
Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm
eLife, Jan 6, 2022; DOI: 10.7554/eLife.67301
Single-cell/-nucleus processing for sc/snRNA-seq
Authors: Rebecca D Hodge and Bosiljka Tasic
Date: Sep 7, 2022，Views：622
Original protocol used in research article：
Single-cell and single-nucleus RNA-seq uncovers shared and distinct axes of variation in dorsal LGN neurons in mice, non-human primates, and humans
eLife, Sep 2, 2021; DOI: 10.7554/eLife.64875
Measures
Author: Andrew S. Huhn
Date: Sep 7, 2022，Views：532
Original protocol used in research article：
Suvorexant ameliorated sleep disturbance, opioid withdrawal, and craving during a buprenorphine taper
Science Translational Medicine, Jun 22, 2022; DOI: 10.1126/scitranslmed.abn8238
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