Protocols shared by authors via RaP service
Journal
2.4. CDC and ADCC assays
Author: Kohtaro Minmi
Date: Nov 27, 2022，Views：988
Original protocol used in research article：
Nonclinical safety evaluation of pabinafusp alfa, an anti-human transferrin receptor antibody and iduronate-2-sulfatase fusion protein, for the treatment of neuronopathic mucopolysaccharidosis type II
Molecular Genetics and Metabolism Reports, Apr 18, 2021; DOI: 10.1016/j.ymgmr.2021.100758
Zebrafish strains
Authors: Yanyan Ding and Feng Liu
Date: Nov 25, 2022，Views：941
Original protocol used in research article：
The chromatin-remodeling enzyme Smarca5 regulates erythrocyte aggregation via Keap1-Nrf2 signaling
eLife, Oct 26, 2021; DOI: 10.7554/eLife.72557
Preparation of exosome and exosomeRNP
Authors: Xiangrui Liu, Yuan Ping, Tianhua Zhou and Tao Wan
Date: Nov 25, 2022，Views：1094
Original protocol used in research article：
Exosome-mediated delivery of Cas9 ribonucleoprotein complexes for tissue-specific gene therapy of liver diseases
Science Advances, Sep 14, 2022; DOI: 10.1126/sciadv.abp9435
Drug release performance
Author: Xu Hou
Date: Nov 25, 2022，Views：819
Original protocol used in research article：
Bioinspired liquid gating membrane-based catheter with anticoagulation and positionally drug release properties
Science Advances, Sep 3, 2020; DOI: 10.1126/sciadv.abb4700
Plasmid constructs and plant transformation
Author: Zuhua He
Date: Nov 25, 2022，Views：919
Original protocol used in research article：
An MKP-MAPK protein phosphorylation cascade controls vascular immunity in plants
Science Advances, Mar 9, 2022; DOI: 10.1126/sciadv.abg8723
2.2. Determination of Total Flavonoids Content
Authors: Xiaoqing Ma, Qiang Ding, Xilin Hou and Xiong You
Date: Nov 24, 2022，Views：776
Original protocol used in research article：
Analysis of Flavonoid Metabolites in Watercress (Nasturtium officinale R. Br.) and the Non-Heading Chinese Cabbage (Brassica rapa ssp. chinensis cv. Aijiaohuang) Using UHPLC-ESI-MS/MS
Molecules, Sep 26, 2021; DOI: 10.3390/molecules26195825
Axenic flies
Authors: Katarina Akhmetova and Igor Chesnokov
Date: Nov 22, 2022，Views：849
Original protocol used in research article：
Drosophila STING protein has a role in lipid metabolism
eLife, Sep 1, 2021; DOI: 10.7554/eLife.67358
Generation of INKBRITE mouse model
Author: Tien Peng
Date: Nov 21, 2022，Views：980
Original protocol used in research article：
Sentinel p16INK4a+ cells in the basement membrane form a reparative niche in the lung
Science, Oct 13, 2022; DOI: 10.1126/science.abf3326
Immuno-SEM
Authors: James R Edgar and Margaret S Robinson
Date: Nov 21, 2022，Views：785
Original protocol used in research article：
Tetherin is an exosomal tether
eLife, Sep 22, 2016; DOI: 10.7554/eLife.17180
V(D)J sequencing
Authors: Marcus James Robinson, David Mathew Tarlinton and Danika Hill
Date: Nov 20, 2022，Views：1095
Original protocol used in research article：
Long-lived plasma cells accumulate in the bone marrow at a constant rate from early in an immune response
Science Immunology, Oct 28, 2022; DOI: 10.1126/sciimmunol.abm8389
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